TY - JOUR

T1 - Development of Antifouling and Bactericidal Coatings for Platelet Storage Bags Using Dopamine Chemistry

AU - Hadjesfandiari, Narges

AU - Weinhart, Marie

AU - Kizhakkedathu, Jayachandran N.

AU - Haag, Rainer

AU - Brooks, Donald E.

N1 - Funding information: The authors thank Dr. Sandra Ramirez-Arcos, Canadian Blood Services, Ottawa for providing the clinical bacterial strains. They also thank Dr. Tae Kyong John Kim, UBC, for collecting the TOF-SIMS data. N.H. thanks Dr. Silke Heinen and Dr. Qiang Wei, Freie Universität Berlin, for their input on QCM-D experiments. This work was supported by Natural Sciences and Engineering Research Council of Canada. The authors benefited from the facilities of the UBC Centre for Blood Research, Department of Chemistry and Department of Materials Engineering. N.H. is a recipient of a Health Canada/Canadian Blood Services graduate fellowship. J.N.K. is a recipient of a Michael Smith Foundation for Health Research Career Scholar Award.

PY - 2018/3/7

Y1 - 2018/3/7

N2 - Platelets have a limited shelf life, due to the risk of bacterial contamination and platelet quality loss. Most platelet storage bags are made of a mixture of polyvinyl chloride with a plasticizer, denoted as pPVC. To improve biocompatibility of pPVC with platelets and to inhibit bacterial biofilm formation, an antifouling polymer coating is developed using mussel-inspired chemistry. A copolymer of N,N-dimethylacrylamide and N-(3-aminopropyl)methacrylamide hydrochloride is synthesized and coupled with catechol groups, named DA51-cat. Under mild aqueous conditions, pPVC is first equilibrated with an anchoring polydopamine layer, followed by a DA51-cat layer. Measurements show this coating decreases fibrinogen adsorption to 5% of the control surfaces. One-step coating with DA51-cat does not coat pPVC efficiently although it is sufficient for coating silicon wafers and gold substrates. The dual layer coating on platelet bags resists bacterial biofilm formation and considerably decreases platelet adhesion. A cationic antimicrobial peptide, E6, is conjugated to DA51-cat then coated on silicon wafers and introduces bactericidal activity to these surfaces. Time-of-flight second ion-mass spectroscopy is successfully applied to characterize these surfaces. pPVC is widely used in medical devices; this method provides an approach to controlling biofouling and bacterial growth on it without elaborate surface modification procedures.

AB - Platelets have a limited shelf life, due to the risk of bacterial contamination and platelet quality loss. Most platelet storage bags are made of a mixture of polyvinyl chloride with a plasticizer, denoted as pPVC. To improve biocompatibility of pPVC with platelets and to inhibit bacterial biofilm formation, an antifouling polymer coating is developed using mussel-inspired chemistry. A copolymer of N,N-dimethylacrylamide and N-(3-aminopropyl)methacrylamide hydrochloride is synthesized and coupled with catechol groups, named DA51-cat. Under mild aqueous conditions, pPVC is first equilibrated with an anchoring polydopamine layer, followed by a DA51-cat layer. Measurements show this coating decreases fibrinogen adsorption to 5% of the control surfaces. One-step coating with DA51-cat does not coat pPVC efficiently although it is sufficient for coating silicon wafers and gold substrates. The dual layer coating on platelet bags resists bacterial biofilm formation and considerably decreases platelet adhesion. A cationic antimicrobial peptide, E6, is conjugated to DA51-cat then coated on silicon wafers and introduces bactericidal activity to these surfaces. Time-of-flight second ion-mass spectroscopy is successfully applied to characterize these surfaces. pPVC is widely used in medical devices; this method provides an approach to controlling biofouling and bacterial growth on it without elaborate surface modification procedures.

KW - antifouling surfaces

KW - antimicrobial peptides

KW - mussel-inspired chemistry

KW - platelet and bacterial adhesion

KW - polyvinyl chloride platelet storage bags

UR - http://www.scopus.com/inward/record.url?scp=85030330565&partnerID=8YFLogxK

U2 - 10.1002/adhm.201700839

DO - 10.1002/adhm.201700839

M3 - Article

C2 - 28961393

AN - SCOPUS:85030330565

VL - 7

JO - Advanced healthcare materials

JF - Advanced healthcare materials

SN - 2192-2640

IS - 5

M1 - 1700839

ER -