TY - JOUR

T1 - Development of an Aptamer-Based Lateral Flow Assay for the Detection of C-Reactive Protein Using Microarray Technology as a Prescreening Platform

AU - Phung, Ngoc Linh

AU - Walter, Johanna G.

AU - Jonczyk, Rebecca

AU - Seiler, Lisa K.

AU - Scheper, Thomas

AU - Blume, Cornelia

N1 - Funding Information: This work has been carried out within the framework of the SMART-BIOTECS alliance between the Technische Universität Braunschweig and the Leibniz Universität Hannover. This initiative is supported by the Ministry of Science and Culture (MWK) of Lower Saxony, Germany. Furthermore, this work was supported by the state of Lower Sayxony and the “Europäischer Fonds für regionale Entwicklung” (EFRE; Grant ZW6-85006385).

PY - 2020/11/9

Y1 - 2020/11/9

N2 - For improved cost-effectiveness and temperature-stability, a ready to use lateral flow assay (LFA) is developed in this work for detecting inflammation/infection biomarker C-reactive protein (CRP) in human patient samples on the basis of aptamers. In prescreening investigations, an aptamer with CRP affinity was immobilized on microarray chips in forward and sandwich formats to optimize assay conditions. We suggest these microarray techniques as a resource-sparing and fast-screening instrument for evaluation of various conditions. The capability of the aptamer to detect CRP was shown. Optimized assay conditions were consequently transferred to the LFA-platform. Here we could demonstrate for the first time an aptamer-based LFA for the detection of CRP in human patient samples in pathologically relevant concentrations. The cutoff for CRP detection is set at 10 mg/L, providing a distinctive "yes"(≥10 mg/L CRP) or "no"(<10 mg/L CRP) answer for the patient. The resulting aptamer-based LFA is promising with regard to its application as point-of-care testing (POCT) for efficient monitoring, especially of patients affected by frequent infections or inflammations.

AB - For improved cost-effectiveness and temperature-stability, a ready to use lateral flow assay (LFA) is developed in this work for detecting inflammation/infection biomarker C-reactive protein (CRP) in human patient samples on the basis of aptamers. In prescreening investigations, an aptamer with CRP affinity was immobilized on microarray chips in forward and sandwich formats to optimize assay conditions. We suggest these microarray techniques as a resource-sparing and fast-screening instrument for evaluation of various conditions. The capability of the aptamer to detect CRP was shown. Optimized assay conditions were consequently transferred to the LFA-platform. Here we could demonstrate for the first time an aptamer-based LFA for the detection of CRP in human patient samples in pathologically relevant concentrations. The cutoff for CRP detection is set at 10 mg/L, providing a distinctive "yes"(≥10 mg/L CRP) or "no"(<10 mg/L CRP) answer for the patient. The resulting aptamer-based LFA is promising with regard to its application as point-of-care testing (POCT) for efficient monitoring, especially of patients affected by frequent infections or inflammations.

KW - aptamer-based LFA

KW - aptamers

KW - CRP

KW - lateral flow assay

KW - microarray

KW - POCT

UR - http://www.scopus.com/inward/record.url?scp=85091414128&partnerID=8YFLogxK

U2 - 10.1021/acscombsci.0c00080

DO - 10.1021/acscombsci.0c00080

M3 - Article

C2 - 32894679

AN - SCOPUS:85091414128

VL - 22

SP - 617

EP - 629

JO - ACS combinatorial science

JF - ACS combinatorial science

SN - 2156-8952

IS - 11

ER -