Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 87-93 |
| Seitenumfang | 7 |
| Fachzeitschrift | Journal of biotechnology |
| Jahrgang | 150 |
| Ausgabenummer | 1 |
| Publikationsstatus | Veröffentlicht - 16 Juli 2010 |
Abstract
The automated monitoring of cell variables in cultivation processes is a key technology in modern bioscience. In this article an innovative analytical tool for monitoring of cell densities in shaking-flask cultivations - consisting of a flow-through microscope with an automated image analysis software integrated in a FIA sampling system - is presented. This atline multitesting system was optimized by varying the height of the microscopes sampling zone. A calibration of the system was performed by correlating the FIA result peaks to known concentrations of Baker's yeast. It was successfully applied in cell density monitoring of cultivation processes of Saccharomyces cerevisiae with a good correlation with offline methods, and further used to monitor 3 parallel cultivations. This methodology was successfully transferred to Bacillus megaterium cultures and applied to measure the cell densities of parallel cultivation setups of B. megaterium and S. cerevisiae.
ASJC Scopus Sachgebiete
- Biochemie, Genetik und Molekularbiologie (insg.)
- Biotechnologie
- Chemische Verfahrenstechnik (insg.)
- Bioengineering
- Immunologie und Mikrobiologie (insg.)
- Angewandte Mikrobiologie und Biotechnologie
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in: Journal of biotechnology, Jahrgang 150, Nr. 1, 16.07.2010, S. 87-93.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Development of a flow-through microscopic multitesting system for parallel monitoring of cell samples in biotechnological cultivation processes
AU - Rehbock, Christoph
AU - Riechers, Daniel
AU - Höpfner, Tim
AU - Bluma, Arne
AU - Lindner, Patrick
AU - Hitzmann, Bernd
AU - Beutel, Sascha
AU - Scheper, Thomas
PY - 2010/7/16
Y1 - 2010/7/16
N2 - The automated monitoring of cell variables in cultivation processes is a key technology in modern bioscience. In this article an innovative analytical tool for monitoring of cell densities in shaking-flask cultivations - consisting of a flow-through microscope with an automated image analysis software integrated in a FIA sampling system - is presented. This atline multitesting system was optimized by varying the height of the microscopes sampling zone. A calibration of the system was performed by correlating the FIA result peaks to known concentrations of Baker's yeast. It was successfully applied in cell density monitoring of cultivation processes of Saccharomyces cerevisiae with a good correlation with offline methods, and further used to monitor 3 parallel cultivations. This methodology was successfully transferred to Bacillus megaterium cultures and applied to measure the cell densities of parallel cultivation setups of B. megaterium and S. cerevisiae.
AB - The automated monitoring of cell variables in cultivation processes is a key technology in modern bioscience. In this article an innovative analytical tool for monitoring of cell densities in shaking-flask cultivations - consisting of a flow-through microscope with an automated image analysis software integrated in a FIA sampling system - is presented. This atline multitesting system was optimized by varying the height of the microscopes sampling zone. A calibration of the system was performed by correlating the FIA result peaks to known concentrations of Baker's yeast. It was successfully applied in cell density monitoring of cultivation processes of Saccharomyces cerevisiae with a good correlation with offline methods, and further used to monitor 3 parallel cultivations. This methodology was successfully transferred to Bacillus megaterium cultures and applied to measure the cell densities of parallel cultivation setups of B. megaterium and S. cerevisiae.
KW - Bacillus megaterium
KW - Cell density
KW - Flow-injection analysis (FIA)
KW - Flow-through microscope
KW - Multitester
KW - Saccharomyces cerevisiae
UR - http://www.scopus.com/inward/record.url?scp=77957366843&partnerID=8YFLogxK
U2 - 10.1016/j.jbiotec.2010.07.014
DO - 10.1016/j.jbiotec.2010.07.014
M3 - Article
C2 - 20638423
AN - SCOPUS:77957366843
VL - 150
SP - 87
EP - 93
JO - Journal of biotechnology
JF - Journal of biotechnology
SN - 0168-1656
IS - 1
ER -