TY - JOUR

T1 - Development and Characterization of a Porcine Mitral Valve Scaffold for Tissue Engineering

AU - Granados, M.

AU - Morticelli, L.

AU - Andriopoulou, S.

AU - Kalozoumis, P.

AU - Pflaum, M.

AU - Iablonskii, P.

AU - Glasmacher, Birgit

AU - Harder, M.

AU - Hegermann, Jan

AU - Wrede, C.

AU - Tudorache, I.

AU - Cebotari, S.

AU - Hilfiker, A.

AU - Haverich, A.

AU - Korossis, Sotirios

N1 - Funding Information: Acknowledgements The authors would like to acknowledge the contribution of Karin Burgwitz and Annemarie Beck at the Lower Saxony Centre for Biomedical Engineering Implant Research and Development for their technical support on histological and immunohistochemical analysis. This research was supported by the People Programme (Marie Curie Actions) of the EU 7th Framework Programme FP7/2007–2013/ under the REA grant agreement number 317512, and the German Research Foundation through the Cluster of Excellence REBIRTH (From Regenerative Biology to Reconstructive Therapy; EXC 62). Funding Information: The authors would like to acknowledge the contribution of Karin Burgwitz and Annemarie Beck at the Lower Saxony Centre for Biomedical Engineering Implant Research and Development for their technical support on histological and immunohistochemical analysis. This research was supported by the People Programme (Marie Curie Actions) of the EU 7th Framework Programme FP7/2007?2013/ under the REA grant agreement number 317512, and the German Research Foundation through the Cluster of Excellence REBIRTH (From Regenerative Biology to Reconstructive Therapy; EXC 62). Publisher Copyright: © 2017, Springer Science+Business Media New York. Copyright: Copyright 2018 Elsevier B.V., All rights reserved.

PY - 2017/8

Y1 - 2017/8

N2 - Decellularized scaffolds represent a promising alternative for mitral valve (MV) replacement. This work developed and characterized a protocol for the decellularization of whole MVs. Porcine MVs were decellularized with 0.5% (w/v) SDS and 0.5% (w/v) SD and sterilized with 0.1% (v/v) PAA. Decellularized samples were seeded with human foreskin fibroblasts and human adipose-derived stem cells to investigate cellular repopulation and infiltration, and with human colony-forming endothelial cells to investigate collagen IV formation. Histology revealed an acellular scaffold with a generally conserved histoarchitecture, but collagen IV loss. Following decellularization, no significant changes were observed in the hydroxyproline content, but there was a significant reduction in the glycosaminoglycan content. SEM/TEM analysis confirmed cellular removal and loss of some extracellular matrix components. Collagen and elastin were generally preserved. The endothelial cells produced newly formed collagen IV on the non-cytotoxic scaffold. The protocol produced acellular scaffolds with generally preserved histoarchitecture, biochemistry, and biomechanics.

AB - Decellularized scaffolds represent a promising alternative for mitral valve (MV) replacement. This work developed and characterized a protocol for the decellularization of whole MVs. Porcine MVs were decellularized with 0.5% (w/v) SDS and 0.5% (w/v) SD and sterilized with 0.1% (v/v) PAA. Decellularized samples were seeded with human foreskin fibroblasts and human adipose-derived stem cells to investigate cellular repopulation and infiltration, and with human colony-forming endothelial cells to investigate collagen IV formation. Histology revealed an acellular scaffold with a generally conserved histoarchitecture, but collagen IV loss. Following decellularization, no significant changes were observed in the hydroxyproline content, but there was a significant reduction in the glycosaminoglycan content. SEM/TEM analysis confirmed cellular removal and loss of some extracellular matrix components. Collagen and elastin were generally preserved. The endothelial cells produced newly formed collagen IV on the non-cytotoxic scaffold. The protocol produced acellular scaffolds with generally preserved histoarchitecture, biochemistry, and biomechanics.

KW - Biochemistry

KW - Biocompatibility

KW - Biomechanics

KW - Collagen IV

KW - Cytotoxicity

KW - Decellularization

KW - Heart valve replacement

KW - Histology

KW - Human adipose-derived stem cells

KW - Human colony-forming endothelial cells

KW - Human foreskin fibroblasts

KW - Immunohistochemistry

KW - Mitral valve

KW - Scaffold

KW - Scaffold seeding

KW - Scanning electron microscopy

KW - Tissue engineering

KW - Transmission electron microscopy

KW - Xenoepitope

KW - α-Gal

UR - http://www.scopus.com/inward/record.url?scp=85018361266&partnerID=8YFLogxK

U2 - 10.1007/s12265-017-9747-z

DO - 10.1007/s12265-017-9747-z

M3 - Article

C2 - 28462436

AN - SCOPUS:85018361266

VL - 10

SP - 374

EP - 390

JO - Journal of Cardiovascular Translational Research

JF - Journal of Cardiovascular Translational Research

SN - 1937-5387

IS - 4

ER -