Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 301-311 |
| Seitenumfang | 11 |
| Fachzeitschrift | Journal of the Mechanical Behavior of Biomedical Materials |
| Jahrgang | 60 |
| Publikationsstatus | Veröffentlicht - 2016 |
Abstract
Working towards establishing a biohybrid lung with optimized hemocompatibility, this study analyzed the feasibility of establishing flow-resistant endothelium on heparin/albumin coated poly-4-methly-1-pentene hollow fiber gas exchange membranes (PMP-HFs). The seeding efficiency and proliferation of human cord blood derived endothelial cells (HCBEC) on PMP-HFs were analyzed under static conditions by WST-8 cell proliferation assay and fluorescence microscopy. The HCBEC monolayer integrity under different flow conditions was also assessed. Endothelial-specific phenotype verification, expression activation levels and thrombogenic state markers were quantified by real-time RT-PCR for cell-to-PMP-HF contact under static and dynamic conditions. The results demonstrated the feasibility of establishing a viable, confluent, and flow-resistant endothelial monolayer on the blood-contact surface of PMP-HFs, which maintained a physiological response to TNFα-stimulation and flow conditions. The endothelial phenotype, expression levels of adhesion molecules and thrombogenic state markers were unaffected by cell-to-PMP-HFs contact. These results represent a significant step towards establishing a biohybrid lung.
ASJC Scopus Sachgebiete
- Werkstoffwissenschaften (insg.)
- Biomaterialien
- Ingenieurwesen (insg.)
- Biomedizintechnik
- Ingenieurwesen (insg.)
- Werkstoffmechanik
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in: Journal of the Mechanical Behavior of Biomedical Materials, Jahrgang 60, 2016, S. 301-311.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Developing a biohybrid lung - sufficient endothelialization of poly-4-methly-1-pentene gas exchange hollow-fiber membranes
AU - Wiegmann, Bettina
AU - Von Seggern, Heide
AU - Höffler, Klaus
AU - Korossis, Sotirios
AU - Dipresa, Daniele
AU - Pflaum, Michael
AU - Schmeckebier, Sabrina
AU - Seume, Jörg
AU - Haverich, Axel
N1 - Funding information: The authors are grateful to Julia Weder for her excellent technical assistance and Nina McGuinness for proofreading the manuscript. This work was partly supported by funding by the Cluster of Excellence REBIRTH (From Regenerative Biology to Reconstructive Therapy EXC 62/1) (Unit 4.1), as well as by the German Center for Lung Research (DZL) (DZL: 82DZL00201) and the German Research Foundation (WI 4088/1-2).The work was awarded with the ASAIO Y Nosé International Fellowship of the American Society for Artificial Internal Organs and the travel scholarship of the German Transplantation Society.
PY - 2016
Y1 - 2016
N2 - Working towards establishing a biohybrid lung with optimized hemocompatibility, this study analyzed the feasibility of establishing flow-resistant endothelium on heparin/albumin coated poly-4-methly-1-pentene hollow fiber gas exchange membranes (PMP-HFs). The seeding efficiency and proliferation of human cord blood derived endothelial cells (HCBEC) on PMP-HFs were analyzed under static conditions by WST-8 cell proliferation assay and fluorescence microscopy. The HCBEC monolayer integrity under different flow conditions was also assessed. Endothelial-specific phenotype verification, expression activation levels and thrombogenic state markers were quantified by real-time RT-PCR for cell-to-PMP-HF contact under static and dynamic conditions. The results demonstrated the feasibility of establishing a viable, confluent, and flow-resistant endothelial monolayer on the blood-contact surface of PMP-HFs, which maintained a physiological response to TNFα-stimulation and flow conditions. The endothelial phenotype, expression levels of adhesion molecules and thrombogenic state markers were unaffected by cell-to-PMP-HFs contact. These results represent a significant step towards establishing a biohybrid lung.
AB - Working towards establishing a biohybrid lung with optimized hemocompatibility, this study analyzed the feasibility of establishing flow-resistant endothelium on heparin/albumin coated poly-4-methly-1-pentene hollow fiber gas exchange membranes (PMP-HFs). The seeding efficiency and proliferation of human cord blood derived endothelial cells (HCBEC) on PMP-HFs were analyzed under static conditions by WST-8 cell proliferation assay and fluorescence microscopy. The HCBEC monolayer integrity under different flow conditions was also assessed. Endothelial-specific phenotype verification, expression activation levels and thrombogenic state markers were quantified by real-time RT-PCR for cell-to-PMP-HF contact under static and dynamic conditions. The results demonstrated the feasibility of establishing a viable, confluent, and flow-resistant endothelial monolayer on the blood-contact surface of PMP-HFs, which maintained a physiological response to TNFα-stimulation and flow conditions. The endothelial phenotype, expression levels of adhesion molecules and thrombogenic state markers were unaffected by cell-to-PMP-HFs contact. These results represent a significant step towards establishing a biohybrid lung.
KW - Bioartificial Lung
KW - Biohybrid lung
KW - Endothelialization
KW - Gas exchange membranes
UR - http://www.scopus.com/inward/record.url?scp=84959335673&partnerID=8YFLogxK
U2 - 10.1016/j.jmbbm.2016.01.032
DO - 10.1016/j.jmbbm.2016.01.032
M3 - Article
C2 - 26919566
AN - SCOPUS:84959335673
VL - 60
SP - 301
EP - 311
JO - Journal of the Mechanical Behavior of Biomedical Materials
JF - Journal of the Mechanical Behavior of Biomedical Materials
SN - 1751-6161
ER -