Determination of major chlorophyll degradation products

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Autoren

  • Thomas J. Mangos
  • Ralf G. Berger

Organisationseinheiten

Externe Organisationen

  • U.S. Department of Agriculture
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Details

OriginalspracheEnglisch
Seiten (von - bis)345-350
Seitenumfang6
FachzeitschriftEuropean Food Research and Technology
Jahrgang204
Ausgabenummer5
PublikationsstatusVeröffentlicht - 1997

Abstract

An HPLC procedure is described which enables both identification and quantitation of chlorophyll a and chlorophyll b and their major degradation products which can occur during solvent extraction of chloropigments from green plant leaves or during storage and canning of green vegetables. The method has been applied to visualize enzymatic as well as chemical degradation of chloropigments from spinach. Complex mixtures of phytyl esters of chlorophyllides, pheophorbides, pyrochlorophyllides, and pyropheophorbides and their respective hydrolysis products were separated, identified and quantitated in the presence of carotenoids in a process lasting less than 40 min.

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Determination of major chlorophyll degradation products. / Mangos, Thomas J.; Berger, Ralf G.
in: European Food Research and Technology, Jahrgang 204, Nr. 5, 1997, S. 345-350.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Mangos, TJ & Berger, RG 1997, 'Determination of major chlorophyll degradation products', European Food Research and Technology, Jg. 204, Nr. 5, S. 345-350.
Mangos, Thomas J. ; Berger, Ralf G. / Determination of major chlorophyll degradation products. in: European Food Research and Technology. 1997 ; Jahrgang 204, Nr. 5. S. 345-350.
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Download

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T1 - Determination of major chlorophyll degradation products

AU - Mangos, Thomas J.

AU - Berger, Ralf G.

N1 - Funding information: We are grateful to the Fonds der Chemischen Industrie, Frankfurt, and to Dr. Marcus, Geesthacht, for financial support.

PY - 1997

Y1 - 1997

N2 - An HPLC procedure is described which enables both identification and quantitation of chlorophyll a and chlorophyll b and their major degradation products which can occur during solvent extraction of chloropigments from green plant leaves or during storage and canning of green vegetables. The method has been applied to visualize enzymatic as well as chemical degradation of chloropigments from spinach. Complex mixtures of phytyl esters of chlorophyllides, pheophorbides, pyrochlorophyllides, and pyropheophorbides and their respective hydrolysis products were separated, identified and quantitated in the presence of carotenoids in a process lasting less than 40 min.

AB - An HPLC procedure is described which enables both identification and quantitation of chlorophyll a and chlorophyll b and their major degradation products which can occur during solvent extraction of chloropigments from green plant leaves or during storage and canning of green vegetables. The method has been applied to visualize enzymatic as well as chemical degradation of chloropigments from spinach. Complex mixtures of phytyl esters of chlorophyllides, pheophorbides, pyrochlorophyllides, and pyropheophorbides and their respective hydrolysis products were separated, identified and quantitated in the presence of carotenoids in a process lasting less than 40 min.

KW - Chlorophyll

KW - Degradation

KW - HPLC

KW - Spinach (Spinacia oleracea)

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JO - European Food Research and Technology

JF - European Food Research and Technology

SN - 1438-2377

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