Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 905-916 |
| Seitenumfang | 12 |
| Fachzeitschrift | Sensors |
| Jahrgang | 1 |
| Ausgabenummer | 11 |
| Publikationsstatus | Veröffentlicht - 2011 |
Abstract
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in: Sensors, Jahrgang 1, Nr. 11, 2011, S. 905-916.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
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TY - JOUR
T1 - Detection of Single Molecules illuminated by a Light-Emitting Diode
AU - Gerhardt, I.
AU - Mai, L.
AU - Lamas-Linares, A.
AU - Kurtsiefer, C.
PY - 2011
Y1 - 2011
N2 - Optical detection and spectroscopy of single molecules has become an indispensable tool in biological imaging and sensing. The success of this technique is based on intense fluorescence of organic dye molecule under carefully engineered laser illumination. In this paper we demonstrate the optical detection of single molecules on a wide-field microscope with an illumination based on a commercially available green light-emitting diode. The results are compared to laser illumination in the same experimental configuration. The setup and the limiting factors, such as light transfer to the sample, spectral filtering and the resulting signal-to-noise ratio are discussed. A theoretical and an experimental approach to estimate these parameters are presented. The results can be adapted to other single emitter and illumination schemes.
AB - Optical detection and spectroscopy of single molecules has become an indispensable tool in biological imaging and sensing. The success of this technique is based on intense fluorescence of organic dye molecule under carefully engineered laser illumination. In this paper we demonstrate the optical detection of single molecules on a wide-field microscope with an illumination based on a commercially available green light-emitting diode. The results are compared to laser illumination in the same experimental configuration. The setup and the limiting factors, such as light transfer to the sample, spectral filtering and the resulting signal-to-noise ratio are discussed. A theoretical and an experimental approach to estimate these parameters are presented. The results can be adapted to other single emitter and illumination schemes.
KW - Single Molecules
KW - Fluorescence Microscopy
KW - Light-Emitting Diode
KW - LED
KW - Signal to Noise Ratio
KW - Single Photon Detection
M3 - Article
VL - 1
SP - 905
EP - 916
JO - Sensors
JF - Sensors
SN - 1424-3210
IS - 11
ER -