TY - JOUR

T1 - Depletion of the "gamma-type carbonic anhydrase-like" subunits of complex I affects central mitochondrial metabolism in Arabidopsis thaliana

T2 - Corrigendum to “depletion of the “gamma-type carbonic anhydrase-like” subunits of complex I affects central mitochondrial metabolism in Arabidopsis thaliana” (Biochimica et Biophysica Acta - Bioenergetics (2016) 1857(1) (60–71))

AU - Fromm, Steffanie

AU - Göing, Jennifer

AU - Lorenz, Christin

AU - Peterhänsel, Christoph

AU - Braun, Hans Peter

N1 - Funding information: The Arabidopsis ?cal1/cal2i line was kindly provided by Qin Wang, Hunan University, China. We gratefully acknowledge the technical assistance of Dagmar Lewejohann. This work was supported by the Deutsche Forschungsgemeinschaft (DFG), Forschergruppe 1186 (grant Br1829/10–2 ).

PY - 2015/10/19

Y1 - 2015/10/19

N2 - "Gamma-type carbonic anhydrase-like" (CAL) proteins form part of complex I in plants. Together with "gamma carbonic anhydrase" (CA) proteins they form an extra domain which is attached to the membrane arm of complex I on its matrix exposed side. In Arabidopsis two CAL and three CA proteins are present, termed CAL1, CAL2, CA1, CA2 and CA3. It has been proposed that the carbonic anhydrase domain of complex I is involved in a process mediating efficient recycling of mitochondrial CO2 for photosynthetic carbon fixation which is especially important during growth conditions causing increased photorespiration. Depletion of CAL proteins has been shown to significantly affect plant development and photomorphogenesis. To better understand CAL function in plants we here investigated effects of CAL depletion on the mitochondrial compartment. In mutant lines and cell cultures complex I amount was reduced by 90-95% but levels of complexes III and V were unchanged. At the same time, some of the CA transcripts were less abundant. Proteome analysis of CAL depleted cells revealed significant reduction of complex I subunits as well as proteins associated with photorespiration, but increased amounts of proteins participating in amino acid catabolism and stress response reactions. Developmental delay of the mutants was slightly alleviated if plants were cultivated at high CO2. Profiling of selected metabolites revealed defined changes in intermediates of the citric acid cycle and amino acid catabolism. It is concluded that CAL proteins are essential for complex I assembly and that CAL depletion specifically affects central mitochondrial metabolism.

AB - "Gamma-type carbonic anhydrase-like" (CAL) proteins form part of complex I in plants. Together with "gamma carbonic anhydrase" (CA) proteins they form an extra domain which is attached to the membrane arm of complex I on its matrix exposed side. In Arabidopsis two CAL and three CA proteins are present, termed CAL1, CAL2, CA1, CA2 and CA3. It has been proposed that the carbonic anhydrase domain of complex I is involved in a process mediating efficient recycling of mitochondrial CO2 for photosynthetic carbon fixation which is especially important during growth conditions causing increased photorespiration. Depletion of CAL proteins has been shown to significantly affect plant development and photomorphogenesis. To better understand CAL function in plants we here investigated effects of CAL depletion on the mitochondrial compartment. In mutant lines and cell cultures complex I amount was reduced by 90-95% but levels of complexes III and V were unchanged. At the same time, some of the CA transcripts were less abundant. Proteome analysis of CAL depleted cells revealed significant reduction of complex I subunits as well as proteins associated with photorespiration, but increased amounts of proteins participating in amino acid catabolism and stress response reactions. Developmental delay of the mutants was slightly alleviated if plants were cultivated at high CO2. Profiling of selected metabolites revealed defined changes in intermediates of the citric acid cycle and amino acid catabolism. It is concluded that CAL proteins are essential for complex I assembly and that CAL depletion specifically affects central mitochondrial metabolism.

KW - Arabidopsis thaliana

KW - Carbonic anhydrase

KW - Complex I

KW - Mitochondrial metabolism

KW - Proteomics

KW - Respiratory chain

UR - http://www.scopus.com/inward/record.url?scp=84946430430&partnerID=8YFLogxK

U2 - 10.1016/j.bbabio.2015.10.006

DO - 10.1016/j.bbabio.2015.10.006

M3 - Article

C2 - 26482706

AN - SCOPUS:84946430430

VL - 1857

SP - 60

EP - 71

JO - Biochimica et Biophysica Acta - Bioenergetics

JF - Biochimica et Biophysica Acta - Bioenergetics

SN - 0005-2728

IS - 1

ER -