Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 446-451 |
| Seitenumfang | 6 |
| Fachzeitschrift | Reproductive Toxicology |
| Jahrgang | 30 |
| Ausgabenummer | 3 |
| Publikationsstatus | Veröffentlicht - Nov. 2010 |
Abstract
Retinoids are essential in vertebrate reproduction and embryonic development. All-trans-retinoic acid (ATRA) is tightly regulated during these processes. CYP26A1 is mainly responsible for its degradation. To study the role of CYP26A1 during implantation, we applied R115866, a CYP26A1-specific antagonist, to rats during early gestation days (GD). On GD 6.5 and 12 samples were collected and the number of embryos was evaluated. ATRA concentration increased in uterus and serum, mRNA expression of CYP26A1 and CRABP2 increased in the liver, but not in the uterus. Uterine COX1 and 17βHSD mRNA expression was decreased. The number of embryos on GD 12 was not altered in this setting. It can be concluded that uterine expression of the analyzed retinoid-response genes during early gestation is not altered by this R115866 treatment and instead indirectly via ATRA. From our experiment we cannot confirm that ATRA obtains a major influencing role in the regulation of embryonic implantation.
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in: Reproductive Toxicology, Jahrgang 30, Nr. 3, 11.2010, S. 446-451.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - CYP26A1-specific antagonist influence on embryonic implantation, gene expression and endogenous retinoid concentration in rats
AU - Fritzsche, Britta
AU - Schuchardt, Jan Philipp
AU - Schmidt, Anja
AU - Nau, Heinz
AU - Schweigert, Florian J
AU - Rühl, Ralph
N1 - Copyright © 2010 Elsevier Inc. All rights reserved.
PY - 2010/11
Y1 - 2010/11
N2 - Retinoids are essential in vertebrate reproduction and embryonic development. All-trans-retinoic acid (ATRA) is tightly regulated during these processes. CYP26A1 is mainly responsible for its degradation. To study the role of CYP26A1 during implantation, we applied R115866, a CYP26A1-specific antagonist, to rats during early gestation days (GD). On GD 6.5 and 12 samples were collected and the number of embryos was evaluated. ATRA concentration increased in uterus and serum, mRNA expression of CYP26A1 and CRABP2 increased in the liver, but not in the uterus. Uterine COX1 and 17βHSD mRNA expression was decreased. The number of embryos on GD 12 was not altered in this setting. It can be concluded that uterine expression of the analyzed retinoid-response genes during early gestation is not altered by this R115866 treatment and instead indirectly via ATRA. From our experiment we cannot confirm that ATRA obtains a major influencing role in the regulation of embryonic implantation.
AB - Retinoids are essential in vertebrate reproduction and embryonic development. All-trans-retinoic acid (ATRA) is tightly regulated during these processes. CYP26A1 is mainly responsible for its degradation. To study the role of CYP26A1 during implantation, we applied R115866, a CYP26A1-specific antagonist, to rats during early gestation days (GD). On GD 6.5 and 12 samples were collected and the number of embryos was evaluated. ATRA concentration increased in uterus and serum, mRNA expression of CYP26A1 and CRABP2 increased in the liver, but not in the uterus. Uterine COX1 and 17βHSD mRNA expression was decreased. The number of embryos on GD 12 was not altered in this setting. It can be concluded that uterine expression of the analyzed retinoid-response genes during early gestation is not altered by this R115866 treatment and instead indirectly via ATRA. From our experiment we cannot confirm that ATRA obtains a major influencing role in the regulation of embryonic implantation.
KW - Animals
KW - Benzothiazoles/pharmacology
KW - Body Weight/drug effects
KW - Chromatography, High Pressure Liquid
KW - Cytochrome P-450 Enzyme Inhibitors
KW - Cytochrome P-450 Enzyme System
KW - Embryo Implantation/drug effects
KW - Female
KW - Gene Expression/drug effects
KW - Liver/enzymology
KW - Organ Size/drug effects
KW - Pregnancy
KW - Rats
KW - Rats, Wistar
KW - Retinoic Acid 4-Hydroxylase
KW - Retinoids/blood
KW - Reverse Transcriptase Polymerase Chain Reaction
KW - Triazoles/pharmacology
KW - Uterus/enzymology
U2 - 10.1016/j.reprotox.2010.05.005
DO - 10.1016/j.reprotox.2010.05.005
M3 - Article
C2 - 20580668
VL - 30
SP - 446
EP - 451
JO - Reproductive Toxicology
JF - Reproductive Toxicology
SN - 0890-6238
IS - 3
ER -