TY - JOUR

T1 - Cultivation of MC3T3-E1 cells on a newly developed material (Sponceram®) using a rotating bed system bioreactor

AU - Suck, Kirstin

AU - Behr, Larissa

AU - Fischer, Marcel

AU - Hoffmeister, Hans

AU - Van Griensven, Martijn

AU - Stahl, Frank

AU - Scheper, Thomas

AU - Kasper, Cornelia

PY - 2006/12/13

Y1 - 2006/12/13

N2 - The influence of a 3D macroporous scaffold (Sponceram®) on the differentiation process into bone cells was investigated under static conditions in cell culture dishes. Furthermore, cultivations were performed using a new bioreactor system in the presence or absence of bone morphogenetic protein 2 (BMP-2). Preosteoblastic MC3T3-E1 cells were first cultured on Sponceram scaffolds in 96-well dishes using standard medium, differentiation medium and BMP-2 medium. Cell proliferation showed a similar course for all conditions used. Alkaline phosphatase (AP) activity resulted in a maximum at day 5 in the presence of BMP-2. Two bioreactor cultivations were performed in a BIOSTAT® Bplus RBS (rotating bed system) 500 on Sponceram carrier discs. One cultivation was performed using standard medium. The second one was used with the same medium with BMP-2 substituted. Significant calcification of the extracellular matrix in the presence of BMP-2 occurred but even in the absence of BMP-2 mineralization was observed, mRNA expression of collagen I, osteocalcin and bone sialoprotein was detected after both reactor cultivations. This study demonstrates that macroporous Sponceram is suitable for the cultivation and differentiation of MC3T3-E1 cells into the osteoblastic phenotype, The results of the bioreactor cultivation revealed that the scaffold promoted the differentiation process even in the absence of BMP-2.

AB - The influence of a 3D macroporous scaffold (Sponceram®) on the differentiation process into bone cells was investigated under static conditions in cell culture dishes. Furthermore, cultivations were performed using a new bioreactor system in the presence or absence of bone morphogenetic protein 2 (BMP-2). Preosteoblastic MC3T3-E1 cells were first cultured on Sponceram scaffolds in 96-well dishes using standard medium, differentiation medium and BMP-2 medium. Cell proliferation showed a similar course for all conditions used. Alkaline phosphatase (AP) activity resulted in a maximum at day 5 in the presence of BMP-2. Two bioreactor cultivations were performed in a BIOSTAT® Bplus RBS (rotating bed system) 500 on Sponceram carrier discs. One cultivation was performed using standard medium. The second one was used with the same medium with BMP-2 substituted. Significant calcification of the extracellular matrix in the presence of BMP-2 occurred but even in the absence of BMP-2 mineralization was observed, mRNA expression of collagen I, osteocalcin and bone sialoprotein was detected after both reactor cultivations. This study demonstrates that macroporous Sponceram is suitable for the cultivation and differentiation of MC3T3-E1 cells into the osteoblastic phenotype, The results of the bioreactor cultivation revealed that the scaffold promoted the differentiation process even in the absence of BMP-2.

KW - Bioreactor for tissue engineering

KW - Bone morphogenetic protein

KW - MC3T3-E1 cell differentiation

KW - Rotating bed system

KW - Sponceram®

UR - http://www.scopus.com/inward/record.url?scp=33846311391&partnerID=8YFLogxK

U2 - 10.1002/jbm.a.30965

DO - 10.1002/jbm.a.30965

M3 - Article

C2 - 16948142

AN - SCOPUS:33846311391

VL - 80A

SP - 268

EP - 275

JO - Journal of Biomedical Materials Research - Part A

JF - Journal of Biomedical Materials Research - Part A

SN - 0021-9304

IS - 2

ER -