Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 1-8 |
| Seitenumfang | 8 |
| Fachzeitschrift | Plant cell reports |
| Jahrgang | 23 |
| Ausgabenummer | 1-2 |
| Publikationsstatus | Veröffentlicht - 25 Mai 2004 |
Abstract
We have developed a simple protocol for the cryopreservation of embryogenic suspension cultures of Cyclamen persicum. Embryogenic suspension cultures in the linear growth phase 7-10 days after subculture were used for cryopreservation. Of the different cryoprotectants tested during a 2-day pre-culture, 0.6 M sucrose resulted in the highest re-growth rates of 75%. An additional pre-treatment with 0.6 M sucrose and 10% DMSO (dimethylsulfoxide) for 1 h also positively affected re-growth. Microscopic studies on viability revealed that only few small embryogenic cells survived cryopreservation, while vacuolated single cells died. Experiments in which the duration of the pre-culture period - i.e. the length of time the embryogenic suspension cells were exposed to 0.6 M sucrose - was varied showed that 2-4 days was the most optimal exposure time to 0.6 M sucrose. Callus re-grown after cryopreservation showed growth rates similar to that of unfrozen callus and regenerated even higher numbers of somatic embryos than unfrozen callus.
ASJC Scopus Sachgebiete
- Agrar- und Biowissenschaften (insg.)
- Agronomie und Nutzpflanzenwissenschaften
- Agrar- und Biowissenschaften (insg.)
- Pflanzenkunde
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in: Plant cell reports, Jahrgang 23, Nr. 1-2, 25.05.2004, S. 1-8.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Cryopreservation of embryogenic suspension cultures of Cyclamen persicum Mill
AU - Winkelmann, Traud
AU - Mußmann, Viola
AU - Serek, Margrethe
PY - 2004/5/25
Y1 - 2004/5/25
N2 - We have developed a simple protocol for the cryopreservation of embryogenic suspension cultures of Cyclamen persicum. Embryogenic suspension cultures in the linear growth phase 7-10 days after subculture were used for cryopreservation. Of the different cryoprotectants tested during a 2-day pre-culture, 0.6 M sucrose resulted in the highest re-growth rates of 75%. An additional pre-treatment with 0.6 M sucrose and 10% DMSO (dimethylsulfoxide) for 1 h also positively affected re-growth. Microscopic studies on viability revealed that only few small embryogenic cells survived cryopreservation, while vacuolated single cells died. Experiments in which the duration of the pre-culture period - i.e. the length of time the embryogenic suspension cells were exposed to 0.6 M sucrose - was varied showed that 2-4 days was the most optimal exposure time to 0.6 M sucrose. Callus re-grown after cryopreservation showed growth rates similar to that of unfrozen callus and regenerated even higher numbers of somatic embryos than unfrozen callus.
AB - We have developed a simple protocol for the cryopreservation of embryogenic suspension cultures of Cyclamen persicum. Embryogenic suspension cultures in the linear growth phase 7-10 days after subculture were used for cryopreservation. Of the different cryoprotectants tested during a 2-day pre-culture, 0.6 M sucrose resulted in the highest re-growth rates of 75%. An additional pre-treatment with 0.6 M sucrose and 10% DMSO (dimethylsulfoxide) for 1 h also positively affected re-growth. Microscopic studies on viability revealed that only few small embryogenic cells survived cryopreservation, while vacuolated single cells died. Experiments in which the duration of the pre-culture period - i.e. the length of time the embryogenic suspension cells were exposed to 0.6 M sucrose - was varied showed that 2-4 days was the most optimal exposure time to 0.6 M sucrose. Callus re-grown after cryopreservation showed growth rates similar to that of unfrozen callus and regenerated even higher numbers of somatic embryos than unfrozen callus.
KW - Long-term storage
KW - Pre-culture
KW - Re-growth
KW - Somatic embryogenesis
UR - http://www.scopus.com/inward/record.url?scp=4344659726&partnerID=8YFLogxK
U2 - 10.1007/s00299-004-0783-1
DO - 10.1007/s00299-004-0783-1
M3 - Article
C2 - 15160283
AN - SCOPUS:4344659726
VL - 23
SP - 1
EP - 8
JO - Plant cell reports
JF - Plant cell reports
SN - 0721-7714
IS - 1-2
ER -