TY - JOUR

T1 - Crosses between monokaryons of Pleurotus sapidus or Pleurotus florida show an improved biotransformation of (+)-valencene to (+)-nootkatone

AU - Omarini, Alejandra B.

AU - Plagemann, Ina

AU - Schimanski, Silke

AU - Krings, Ulrich

AU - Berger, Ralf G.

N1 - Funding information: A.O. was supported by DAAD Postdoctoral Fellowship and a young researchers stipendium from the Gottfried Wilhelm Leibniz Universität , Hannover.

PY - 2014/8/22

Y1 - 2014/8/22

N2 - Several hundred monokaryotic and new dikaryotic strains derived thereof were established from (+)-valencene tolerant Pleurotus species. When grouped according to their growth rate on agar plates and compared to the parental of Pleurotus sapidus 69, the slowly growing monokaryons converted (+)-valencene more efficiently to the grapefruit flavour compound (+)-nootkatone. The fast growing monokaryons and the slow × slow and the fast. ×. fast dikaryotic crosses showed similar or inferior yields. Some slow × fast dikaryons, however, exceeded the biotransformation capability of the parental dikaryon significantly. The activity of the responsible enzyme, lipoxygenase, showed a weak correlation with the yields of (+)-nootkatone indicating that the determination of enzyme activity using the primary substrate linoleic acid may be misleading in predicting the biotransformation efficiency. This exploratory study indicated that a classical genetics approach resulted in altered and partly improved terpene transformation capability (plus 60%) and lipoxygenase activity of the strains.

AB - Several hundred monokaryotic and new dikaryotic strains derived thereof were established from (+)-valencene tolerant Pleurotus species. When grouped according to their growth rate on agar plates and compared to the parental of Pleurotus sapidus 69, the slowly growing monokaryons converted (+)-valencene more efficiently to the grapefruit flavour compound (+)-nootkatone. The fast growing monokaryons and the slow × slow and the fast. ×. fast dikaryotic crosses showed similar or inferior yields. Some slow × fast dikaryons, however, exceeded the biotransformation capability of the parental dikaryon significantly. The activity of the responsible enzyme, lipoxygenase, showed a weak correlation with the yields of (+)-nootkatone indicating that the determination of enzyme activity using the primary substrate linoleic acid may be misleading in predicting the biotransformation efficiency. This exploratory study indicated that a classical genetics approach resulted in altered and partly improved terpene transformation capability (plus 60%) and lipoxygenase activity of the strains.

KW - Basidiomycota

KW - Biotransformation

KW - Monokaryon

KW - Pleurotus

UR - http://www.scopus.com/inward/record.url?scp=84906841696&partnerID=8YFLogxK

U2 - 10.1016/j.biortech.2014.08.061

DO - 10.1016/j.biortech.2014.08.061

M3 - Article

C2 - 25189516

AN - SCOPUS:84906841696

VL - 171

SP - 113

EP - 119

JO - Bioresource technology

JF - Bioresource technology

SN - 0960-8524

ER -