Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 221-226 |
| Seitenumfang | 6 |
| Fachzeitschrift | Analytica chimica acta |
| Jahrgang | 190 |
| Ausgabenummer | C |
| Publikationsstatus | Veröffentlicht - 1986 |
Abstract
For optimal cultivation of recombinant E. coli cells, it is necessary to monitor the intracellular enzyme activity by a fast, reliable and simple method. A modified air-segmented flow system is described for the on-line determination of intracellular penicillin-G-acylase (E.C. 3.5.1.11) during the cultivation of genetically modified E. coli 5K(pHM12). The results are compared to those obtained by different conventional off-line techniques.
ASJC Scopus Sachgebiete
- Chemie (insg.)
- Analytische Chemie
- Biochemie, Genetik und Molekularbiologie (insg.)
- Biochemie
- Umweltwissenschaften (insg.)
- Umweltchemie
- Chemie (insg.)
- Spektroskopie
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in: Analytica chimica acta, Jahrgang 190, Nr. C, 1986, S. 221-226.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Continuous on-line monitoring of intracellular enzyme activity
AU - Ahlmann, N.
AU - Niehoff, A.
AU - Rinas, U.
AU - Scheper, Th
AU - Schügerl, K.
PY - 1986
Y1 - 1986
N2 - For optimal cultivation of recombinant E. coli cells, it is necessary to monitor the intracellular enzyme activity by a fast, reliable and simple method. A modified air-segmented flow system is described for the on-line determination of intracellular penicillin-G-acylase (E.C. 3.5.1.11) during the cultivation of genetically modified E. coli 5K(pHM12). The results are compared to those obtained by different conventional off-line techniques.
AB - For optimal cultivation of recombinant E. coli cells, it is necessary to monitor the intracellular enzyme activity by a fast, reliable and simple method. A modified air-segmented flow system is described for the on-line determination of intracellular penicillin-G-acylase (E.C. 3.5.1.11) during the cultivation of genetically modified E. coli 5K(pHM12). The results are compared to those obtained by different conventional off-line techniques.
UR - http://www.scopus.com/inward/record.url?scp=38249041388&partnerID=8YFLogxK
U2 - 10.1016/S0003-2670(00)82883-4
DO - 10.1016/S0003-2670(00)82883-4
M3 - Article
AN - SCOPUS:38249041388
VL - 190
SP - 221
EP - 226
JO - Analytica chimica acta
JF - Analytica chimica acta
SN - 0003-2670
IS - C
ER -