Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 204-210 |
| Seitenumfang | 7 |
| Fachzeitschrift | Microscopy and Microanalysis |
| Jahrgang | 13 |
| Ausgabenummer | 3 |
| Publikationsstatus | Veröffentlicht - 9 Mai 2007 |
Abstract
Heart development, especially the critical phase of cardiac looping, is a complex and intricate process that has not yet been visualized "live" over long periods of time. We have constructed and established a new environmental incubator chamber that provides stable conditions for embryonic development with regard to temperature, humidity, and oxygen levels. We have integrated a video microscope in the chamber to visualize the developing heart in real time and present the first "live" recordings of a chick embryo in shell-less culture acquired over a period of 2 days. The time-lapse images we show depict a significant time window that covers the most critical and typical morphogenetic events during normal cardiac looping. Our system is of interest to researchers in the field of embryogenesis, as it can be adapted to a variety of animal models for organogenesis studies including heart and limb development.
ASJC Scopus Sachgebiete
- Physik und Astronomie (insg.)
- Instrumentierung
Zitieren
- Standard
- Harvard
- Apa
- Vancouver
- BibTex
- RIS
in: Microscopy and Microanalysis, Jahrgang 13, Nr. 3, 09.05.2007, S. 204-210.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Construction and Establishment of a New Environmental Chamber to Study Real-Time Cardiac Development
AU - Orhan, Gülay
AU - Baron, Stephan
AU - Norozi, Kambiz
AU - Männer, Jörg
AU - Hornung, Oliver
AU - Blume, Holger
AU - Misske, Judith
AU - Heimann, Bodo
AU - Wessel, Armin
AU - Mesud Yelbuz, T.
PY - 2007/5/9
Y1 - 2007/5/9
N2 - Heart development, especially the critical phase of cardiac looping, is a complex and intricate process that has not yet been visualized "live" over long periods of time. We have constructed and established a new environmental incubator chamber that provides stable conditions for embryonic development with regard to temperature, humidity, and oxygen levels. We have integrated a video microscope in the chamber to visualize the developing heart in real time and present the first "live" recordings of a chick embryo in shell-less culture acquired over a period of 2 days. The time-lapse images we show depict a significant time window that covers the most critical and typical morphogenetic events during normal cardiac looping. Our system is of interest to researchers in the field of embryogenesis, as it can be adapted to a variety of animal models for organogenesis studies including heart and limb development.
AB - Heart development, especially the critical phase of cardiac looping, is a complex and intricate process that has not yet been visualized "live" over long periods of time. We have constructed and established a new environmental incubator chamber that provides stable conditions for embryonic development with regard to temperature, humidity, and oxygen levels. We have integrated a video microscope in the chamber to visualize the developing heart in real time and present the first "live" recordings of a chick embryo in shell-less culture acquired over a period of 2 days. The time-lapse images we show depict a significant time window that covers the most critical and typical morphogenetic events during normal cardiac looping. Our system is of interest to researchers in the field of embryogenesis, as it can be adapted to a variety of animal models for organogenesis studies including heart and limb development.
KW - Cardiac looping
KW - Heart development
KW - Imaging
UR - http://www.scopus.com/inward/record.url?scp=34248332615&partnerID=8YFLogxK
U2 - 10.1017/S1431927607070390
DO - 10.1017/S1431927607070390
M3 - Article
C2 - 17490503
AN - SCOPUS:34248332615
VL - 13
SP - 204
EP - 210
JO - Microscopy and Microanalysis
JF - Microscopy and Microanalysis
SN - 1431-9276
IS - 3
ER -