Details
| Originalsprache | Englisch |
|---|---|
| Aufsatznummer | 025001 |
| Seitenumfang | 15 |
| Fachzeitschrift | BIOFABRICATION |
| Jahrgang | 8 |
| Ausgabenummer | 2 |
| Publikationsstatus | Veröffentlicht - 14 Apr. 2016 |
Abstract
Biofabrication is attracting interest as a means to produce nanostructured functional materials because of its operational versatility and full scalability. Materials based on proteins are especially appealing, as the structure and functionality of proteins can be adapted by genetic engineering. Furthermore, strategies and tools for protein production have been developed and refined steadily for more than 30 years. However, protein conformation and therefore activity might be sensitive to production conditions. Here, we have explored whether the downstream strategy influences the structure and biological activities, in vitro and in vivo, of a self-assembling, CD44-targeted protein-only nanoparticle produced in Escherichia coli. This has been performed through the comparative analysis of particles built from soluble protein species or protein versions obtained by in vitro protein extraction from inclusion bodies, through mild, non-denaturing procedures. These methods have been developed recently as a convenient alternative to the use of toxic chaotropic agents for protein resolubilization from protein aggregates. The results indicate that the resulting material shows substantial differences in its physicochemical properties and its biological performance at the systems level, and that its building blocks are sensitive to the particular protein source.
ASJC Scopus Sachgebiete
- Biochemie, Genetik und Molekularbiologie (insg.)
- Biotechnologie
- Chemische Verfahrenstechnik (insg.)
- Bioengineering
- Biochemie, Genetik und Molekularbiologie (insg.)
- Biochemie
- Werkstoffwissenschaften (insg.)
- Biomaterialien
- Ingenieurwesen (insg.)
- Biomedizintechnik
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in: BIOFABRICATION, Jahrgang 8, Nr. 2, 025001, 14.04.2016.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Conformational and functional variants of CD44-targeted protein nanoparticles bio-produced in bacteria
AU - Pesarrodona, Mireia
AU - Fernández, Yolanda
AU - Foradada, Laia
AU - Sánchez-Chardi, Alejandro
AU - Conchillo-Solé, Oscar
AU - Unzueta, Ugutz
AU - Xu, Zhikun
AU - Roldán, Mónica
AU - Villegas, Sandra
AU - Ferrer-Miralles, Neus
AU - Schwartz, Simó
AU - Rinas, Ursula
AU - Daura, Xavier
AU - Abasolo, Ibane
AU - Vázquez, Esther
AU - Villaverde, Antonio
N1 - Funding Information: We are indebted to MINECO (BIO2013-41019-P), AGAUR (2014SGR-132) and CIBER de Bioingeniera, Biomateriales y Nanomedicina (project NANOPROTHER); to AV and the Marat de TV3 foundation (TV32013-3930); to EV and IA and FIS (PI15/00272); and to EV for funding our research on protein-based therapeutics.
PY - 2016/4/14
Y1 - 2016/4/14
N2 - Biofabrication is attracting interest as a means to produce nanostructured functional materials because of its operational versatility and full scalability. Materials based on proteins are especially appealing, as the structure and functionality of proteins can be adapted by genetic engineering. Furthermore, strategies and tools for protein production have been developed and refined steadily for more than 30 years. However, protein conformation and therefore activity might be sensitive to production conditions. Here, we have explored whether the downstream strategy influences the structure and biological activities, in vitro and in vivo, of a self-assembling, CD44-targeted protein-only nanoparticle produced in Escherichia coli. This has been performed through the comparative analysis of particles built from soluble protein species or protein versions obtained by in vitro protein extraction from inclusion bodies, through mild, non-denaturing procedures. These methods have been developed recently as a convenient alternative to the use of toxic chaotropic agents for protein resolubilization from protein aggregates. The results indicate that the resulting material shows substantial differences in its physicochemical properties and its biological performance at the systems level, and that its building blocks are sensitive to the particular protein source.
AB - Biofabrication is attracting interest as a means to produce nanostructured functional materials because of its operational versatility and full scalability. Materials based on proteins are especially appealing, as the structure and functionality of proteins can be adapted by genetic engineering. Furthermore, strategies and tools for protein production have been developed and refined steadily for more than 30 years. However, protein conformation and therefore activity might be sensitive to production conditions. Here, we have explored whether the downstream strategy influences the structure and biological activities, in vitro and in vivo, of a self-assembling, CD44-targeted protein-only nanoparticle produced in Escherichia coli. This has been performed through the comparative analysis of particles built from soluble protein species or protein versions obtained by in vitro protein extraction from inclusion bodies, through mild, non-denaturing procedures. These methods have been developed recently as a convenient alternative to the use of toxic chaotropic agents for protein resolubilization from protein aggregates. The results indicate that the resulting material shows substantial differences in its physicochemical properties and its biological performance at the systems level, and that its building blocks are sensitive to the particular protein source.
KW - bacteria
KW - cell factories
KW - drug delivery
KW - nanoparticles
KW - protein folding
KW - recombinant proteins
UR - http://www.scopus.com/inward/record.url?scp=84984678292&partnerID=8YFLogxK
U2 - 10.1088/1758-5090/8/2/025001
DO - 10.1088/1758-5090/8/2/025001
M3 - Article
C2 - 27078873
AN - SCOPUS:84984678292
VL - 8
JO - BIOFABRICATION
JF - BIOFABRICATION
SN - 1758-5082
IS - 2
M1 - 025001
ER -