Details
| Originalsprache | Englisch |
|---|---|
| Aufsatznummer | 2714 |
| Fachzeitschrift | Polymers |
| Jahrgang | 15 |
| Ausgabenummer | 12 |
| Publikationsstatus | Veröffentlicht - 17 Juni 2023 |
Abstract
The development of new biodegradable biomaterials with osteoconductive properties for bone tissue regeneration is one of the urgent tasks of modern medicine. In this study, we proposed the pathway for graphene oxide (GO) modification with oligo/poly(glutamic acid) (oligo/poly(Glu)) possessing osteoconductive properties. The modification was confirmed by a number of methods such as Fourier-transform infrared spectroscopy, quantitative amino acid HPLC analysis, thermogravimetric analysis, scanning electron microscopy, and dynamic and electrophoretic light scattering. Modified GO was used as a filler for poly(ε-caprolactone) (PCL) in the fabrication of composite films. The mechanical properties of the biocomposites were compared with those obtained for the PCL/GO composites. An 18–27% increase in elastic modulus was found for all composites containing modified GO. No significant cytotoxicity of the GO and its derivatives in human osteosarcoma cells (MG-63) was revealed. Moreover, the developed composites stimulated the proliferation of human mesenchymal stem cells (hMSCs) adhered to the surface of the films in comparison with unfilled PCL material. The osteoconductive properties of the PCL-based composites filled with GO modified with oligo/poly(Glu) were confirmed via alkaline phosphatase assay as well as calcein and alizarin red S staining after osteogenic differentiation of hMSC in vitro.
ASJC Scopus Sachgebiete
- Chemie (insg.)
- Allgemeine Chemie
- Werkstoffwissenschaften (insg.)
- Polymere und Kunststoffe
Ziele für nachhaltige Entwicklung
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in: Polymers, Jahrgang 15, Nr. 12, 2714, 17.06.2023.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Composites Based on Poly(ε-caprolactone) and Graphene Oxide Modified with Oligo/Poly(Glutamic Acid) as Biomaterials with Osteoconductive Properties
AU - Solomakha, Olga
AU - Stepanova, Mariia
AU - Gofman, Iosif
AU - Nashchekina, Yulia
AU - Rabchinskii, Maxim
AU - Nashchekin, Alexey
AU - Lavrentieva, Antonina
AU - Korzhikova-Vlakh, Evgenia
N1 - Funding Information: The work of authors from IMC RAS was supported by the Ministry of Science and Higher Education of the Russian Federation as part of the State Assignment of IMC RAS (project no. AAAA-A20-120022090039-8). The work of M. Rabchinskii was supported by the Ministry of Science and Higher Education of the Russian Federation (project no. FFUG-2022-0010).
PY - 2023/6/17
Y1 - 2023/6/17
N2 - The development of new biodegradable biomaterials with osteoconductive properties for bone tissue regeneration is one of the urgent tasks of modern medicine. In this study, we proposed the pathway for graphene oxide (GO) modification with oligo/poly(glutamic acid) (oligo/poly(Glu)) possessing osteoconductive properties. The modification was confirmed by a number of methods such as Fourier-transform infrared spectroscopy, quantitative amino acid HPLC analysis, thermogravimetric analysis, scanning electron microscopy, and dynamic and electrophoretic light scattering. Modified GO was used as a filler for poly(ε-caprolactone) (PCL) in the fabrication of composite films. The mechanical properties of the biocomposites were compared with those obtained for the PCL/GO composites. An 18–27% increase in elastic modulus was found for all composites containing modified GO. No significant cytotoxicity of the GO and its derivatives in human osteosarcoma cells (MG-63) was revealed. Moreover, the developed composites stimulated the proliferation of human mesenchymal stem cells (hMSCs) adhered to the surface of the films in comparison with unfilled PCL material. The osteoconductive properties of the PCL-based composites filled with GO modified with oligo/poly(Glu) were confirmed via alkaline phosphatase assay as well as calcein and alizarin red S staining after osteogenic differentiation of hMSC in vitro.
AB - The development of new biodegradable biomaterials with osteoconductive properties for bone tissue regeneration is one of the urgent tasks of modern medicine. In this study, we proposed the pathway for graphene oxide (GO) modification with oligo/poly(glutamic acid) (oligo/poly(Glu)) possessing osteoconductive properties. The modification was confirmed by a number of methods such as Fourier-transform infrared spectroscopy, quantitative amino acid HPLC analysis, thermogravimetric analysis, scanning electron microscopy, and dynamic and electrophoretic light scattering. Modified GO was used as a filler for poly(ε-caprolactone) (PCL) in the fabrication of composite films. The mechanical properties of the biocomposites were compared with those obtained for the PCL/GO composites. An 18–27% increase in elastic modulus was found for all composites containing modified GO. No significant cytotoxicity of the GO and its derivatives in human osteosarcoma cells (MG-63) was revealed. Moreover, the developed composites stimulated the proliferation of human mesenchymal stem cells (hMSCs) adhered to the surface of the films in comparison with unfilled PCL material. The osteoconductive properties of the PCL-based composites filled with GO modified with oligo/poly(Glu) were confirmed via alkaline phosphatase assay as well as calcein and alizarin red S staining after osteogenic differentiation of hMSC in vitro.
KW - biocompatible polymer composites
KW - grafting from
KW - grafting to
KW - graphene oxide
KW - graphene oxide modification
KW - oligomers and polymers of glutamic acid
KW - osteoconductive materials
KW - poly(ε-caprolactone)
UR - http://www.scopus.com/inward/record.url?scp=85163783297&partnerID=8YFLogxK
U2 - 10.3390/polym15122714
DO - 10.3390/polym15122714
M3 - Article
AN - SCOPUS:85163783297
VL - 15
JO - Polymers
JF - Polymers
SN - 2073-4360
IS - 12
M1 - 2714
ER -