Composites Based on Poly(ε-caprolactone) and Graphene Oxide Modified with Oligo/Poly(Glutamic Acid) as Biomaterials with Osteoconductive Properties

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Autoren

  • Olga Solomakha
  • Mariia Stepanova
  • Iosif Gofman
  • Yulia Nashchekina
  • Maxim Rabchinskii
  • Alexey Nashchekin
  • Antonina Lavrentieva
  • Evgenia Korzhikova-Vlakh

Organisationseinheiten

Externe Organisationen

  • Russian Academy of Sciences (RAS)
  • RAS - Institute of Cytology
  • RAS - Ioffe Physico Technical Institute
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Details

OriginalspracheEnglisch
Aufsatznummer2714
FachzeitschriftPolymers
Jahrgang15
Ausgabenummer12
PublikationsstatusVeröffentlicht - 17 Juni 2023

Abstract

The development of new biodegradable biomaterials with osteoconductive properties for bone tissue regeneration is one of the urgent tasks of modern medicine. In this study, we proposed the pathway for graphene oxide (GO) modification with oligo/poly(glutamic acid) (oligo/poly(Glu)) possessing osteoconductive properties. The modification was confirmed by a number of methods such as Fourier-transform infrared spectroscopy, quantitative amino acid HPLC analysis, thermogravimetric analysis, scanning electron microscopy, and dynamic and electrophoretic light scattering. Modified GO was used as a filler for poly(ε-caprolactone) (PCL) in the fabrication of composite films. The mechanical properties of the biocomposites were compared with those obtained for the PCL/GO composites. An 18–27% increase in elastic modulus was found for all composites containing modified GO. No significant cytotoxicity of the GO and its derivatives in human osteosarcoma cells (MG-63) was revealed. Moreover, the developed composites stimulated the proliferation of human mesenchymal stem cells (hMSCs) adhered to the surface of the films in comparison with unfilled PCL material. The osteoconductive properties of the PCL-based composites filled with GO modified with oligo/poly(Glu) were confirmed via alkaline phosphatase assay as well as calcein and alizarin red S staining after osteogenic differentiation of hMSC in vitro.

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Composites Based on Poly(ε-caprolactone) and Graphene Oxide Modified with Oligo/Poly(Glutamic Acid) as Biomaterials with Osteoconductive Properties. / Solomakha, Olga; Stepanova, Mariia; Gofman, Iosif et al.
in: Polymers, Jahrgang 15, Nr. 12, 2714, 17.06.2023.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Solomakha, O, Stepanova, M, Gofman, I, Nashchekina, Y, Rabchinskii, M, Nashchekin, A, Lavrentieva, A & Korzhikova-Vlakh, E 2023, 'Composites Based on Poly(ε-caprolactone) and Graphene Oxide Modified with Oligo/Poly(Glutamic Acid) as Biomaterials with Osteoconductive Properties', Polymers, Jg. 15, Nr. 12, 2714. https://doi.org/10.3390/polym15122714
Solomakha, O., Stepanova, M., Gofman, I., Nashchekina, Y., Rabchinskii, M., Nashchekin, A., Lavrentieva, A., & Korzhikova-Vlakh, E. (2023). Composites Based on Poly(ε-caprolactone) and Graphene Oxide Modified with Oligo/Poly(Glutamic Acid) as Biomaterials with Osteoconductive Properties. Polymers, 15(12), Artikel 2714. https://doi.org/10.3390/polym15122714
Solomakha O, Stepanova M, Gofman I, Nashchekina Y, Rabchinskii M, Nashchekin A et al. Composites Based on Poly(ε-caprolactone) and Graphene Oxide Modified with Oligo/Poly(Glutamic Acid) as Biomaterials with Osteoconductive Properties. Polymers. 2023 Jun 17;15(12):2714. doi: 10.3390/polym15122714
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title = "Composites Based on Poly(ε-caprolactone) and Graphene Oxide Modified with Oligo/Poly(Glutamic Acid) as Biomaterials with Osteoconductive Properties",
abstract = "The development of new biodegradable biomaterials with osteoconductive properties for bone tissue regeneration is one of the urgent tasks of modern medicine. In this study, we proposed the pathway for graphene oxide (GO) modification with oligo/poly(glutamic acid) (oligo/poly(Glu)) possessing osteoconductive properties. The modification was confirmed by a number of methods such as Fourier-transform infrared spectroscopy, quantitative amino acid HPLC analysis, thermogravimetric analysis, scanning electron microscopy, and dynamic and electrophoretic light scattering. Modified GO was used as a filler for poly(ε-caprolactone) (PCL) in the fabrication of composite films. The mechanical properties of the biocomposites were compared with those obtained for the PCL/GO composites. An 18–27% increase in elastic modulus was found for all composites containing modified GO. No significant cytotoxicity of the GO and its derivatives in human osteosarcoma cells (MG-63) was revealed. Moreover, the developed composites stimulated the proliferation of human mesenchymal stem cells (hMSCs) adhered to the surface of the films in comparison with unfilled PCL material. The osteoconductive properties of the PCL-based composites filled with GO modified with oligo/poly(Glu) were confirmed via alkaline phosphatase assay as well as calcein and alizarin red S staining after osteogenic differentiation of hMSC in vitro.",
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note = "Funding Information: The work of authors from IMC RAS was supported by the Ministry of Science and Higher Education of the Russian Federation as part of the State Assignment of IMC RAS (project no. AAAA-A20-120022090039-8). The work of M. Rabchinskii was supported by the Ministry of Science and Higher Education of the Russian Federation (project no. FFUG-2022-0010).",
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T1 - Composites Based on Poly(ε-caprolactone) and Graphene Oxide Modified with Oligo/Poly(Glutamic Acid) as Biomaterials with Osteoconductive Properties

AU - Solomakha, Olga

AU - Stepanova, Mariia

AU - Gofman, Iosif

AU - Nashchekina, Yulia

AU - Rabchinskii, Maxim

AU - Nashchekin, Alexey

AU - Lavrentieva, Antonina

AU - Korzhikova-Vlakh, Evgenia

N1 - Funding Information: The work of authors from IMC RAS was supported by the Ministry of Science and Higher Education of the Russian Federation as part of the State Assignment of IMC RAS (project no. AAAA-A20-120022090039-8). The work of M. Rabchinskii was supported by the Ministry of Science and Higher Education of the Russian Federation (project no. FFUG-2022-0010).

PY - 2023/6/17

Y1 - 2023/6/17

N2 - The development of new biodegradable biomaterials with osteoconductive properties for bone tissue regeneration is one of the urgent tasks of modern medicine. In this study, we proposed the pathway for graphene oxide (GO) modification with oligo/poly(glutamic acid) (oligo/poly(Glu)) possessing osteoconductive properties. The modification was confirmed by a number of methods such as Fourier-transform infrared spectroscopy, quantitative amino acid HPLC analysis, thermogravimetric analysis, scanning electron microscopy, and dynamic and electrophoretic light scattering. Modified GO was used as a filler for poly(ε-caprolactone) (PCL) in the fabrication of composite films. The mechanical properties of the biocomposites were compared with those obtained for the PCL/GO composites. An 18–27% increase in elastic modulus was found for all composites containing modified GO. No significant cytotoxicity of the GO and its derivatives in human osteosarcoma cells (MG-63) was revealed. Moreover, the developed composites stimulated the proliferation of human mesenchymal stem cells (hMSCs) adhered to the surface of the films in comparison with unfilled PCL material. The osteoconductive properties of the PCL-based composites filled with GO modified with oligo/poly(Glu) were confirmed via alkaline phosphatase assay as well as calcein and alizarin red S staining after osteogenic differentiation of hMSC in vitro.

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KW - grafting from

KW - grafting to

KW - graphene oxide

KW - graphene oxide modification

KW - oligomers and polymers of glutamic acid

KW - osteoconductive materials

KW - poly(ε-caprolactone)

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DO - 10.3390/polym15122714

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JO - Polymers

JF - Polymers

SN - 2073-4360

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ER -

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