Complete genome sequence and construction of an infectious full-length cDNA clone of a German isolate of celery mosaic virus

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OriginalspracheEnglisch
Seiten (von - bis)1107-1111
Seitenumfang5
FachzeitschriftArchives of virology
Jahrgang163
Ausgabenummer4
PublikationsstatusVeröffentlicht - Apr. 2018

Abstract

The complete genome sequence of a German isolate of celery mosaic virus (CeMV, a potyvirus) from Quedlinburg (DSMZ PV-1003) was determined (MF962880). This represents the second fully sequenced genome of this virus, along with a Californian isolate (HQ676607.1). The positive-sense single-stranded RNA is 10,000 nucleotides in length and shows the typical organization of potyviruses but has a shorter PIPO than CeMV California. In comparison to CeMV isolates from different origins, CeMV-Quedlinburg and isolates from the Netherlands (AF203531.1) and Aschersleben, Germany (AJ271087.1) show a NAG instead of DAG in the region of the coat protein responsible for aphid transmission. In this study the first infectious full-length clone of celery mosaic virus was obtained and the infectivity confirmed by Rhizobium radiobacter infiltration of Apium species.

ASJC Scopus Sachgebiete

  • Immunologie und Mikrobiologie (insg.)
  • Virologie

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Complete genome sequence and construction of an infectious full-length cDNA clone of a German isolate of celery mosaic virus. / Rose, Hanna; Maiss, Edgar.
in: Archives of virology, Jahrgang 163, Nr. 4, 04.2018, S. 1107-1111.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

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