Competence in Bacillus subtilis is controlled by regulated proteolysis of a transcription factor

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Autoren

  • Kürşad Turgay
  • Jeanette Hahn
  • Jan Burghoorn
  • David Dubnau

Externe Organisationen

  • Public Health Research Institute - New York
  • Erasmus University Rotterdam
Forschungs-netzwerk anzeigen

Details

OriginalspracheEnglisch
Seiten (von - bis)6730-6738
Seitenumfang9
FachzeitschriftEMBO Journal
Jahrgang17
Ausgabenummer22
PublikationsstatusVeröffentlicht - 16 Nov. 1998
Extern publiziertJa

Abstract

Competence is a physiological state, distinct from sporulation and vegetative growth, that enables cells to bind and internalize transforming DNA. The transcriptional regulator ComK drives the development of competence in Bacillus subtilis. ComK is directly required for its own transcription as well as for the transcription of the genes that encode DNA transport proteins. When ComK is sequestered by binding to a complex of the proteins MecA and ClpC, the positive feedback loop leading to ComK synthesis is interrupted. The small protein ComS, produced as a result of signaling by a quorum-sensing two-component regulatory pathway, triggers the release of ComK from the complex, enabling comK transcription to occur. We show here, based on in vivo and in vitro experiments, that ComK accumulation is also regulated by proteolysis and that binding to MecA targets ComK for degradation by the ClpP protease in association with ClpC. The release of ComK from binding by MecA and ClpC, which occurs when ComS is synthesized, protects ComK from proteolysis. Following this release, the rates of MecA and ComS degradation by ClpCP are increased in our in vitro system. In this novel system, MecA serves to recruit ComK to the ClpCP protease and connects ComK degradation to the quorum-sensing signal-transduction pathway, thereby regulating a key developmental process. This is the first regulated degradation system in which a specific targeting molecule serves such a function.

ASJC Scopus Sachgebiete

Zitieren

Competence in Bacillus subtilis is controlled by regulated proteolysis of a transcription factor. / Turgay, Kürşad; Hahn, Jeanette; Burghoorn, Jan et al.
in: EMBO Journal, Jahrgang 17, Nr. 22, 16.11.1998, S. 6730-6738.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Turgay, K, Hahn, J, Burghoorn, J & Dubnau, D 1998, 'Competence in Bacillus subtilis is controlled by regulated proteolysis of a transcription factor', EMBO Journal, Jg. 17, Nr. 22, S. 6730-6738.
Turgay, K., Hahn, J., Burghoorn, J., & Dubnau, D. (1998). Competence in Bacillus subtilis is controlled by regulated proteolysis of a transcription factor. EMBO Journal, 17(22), 6730-6738.
Turgay K, Hahn J, Burghoorn J, Dubnau D. Competence in Bacillus subtilis is controlled by regulated proteolysis of a transcription factor. EMBO Journal. 1998 Nov 16;17(22):6730-6738.
Turgay, Kürşad ; Hahn, Jeanette ; Burghoorn, Jan et al. / Competence in Bacillus subtilis is controlled by regulated proteolysis of a transcription factor. in: EMBO Journal. 1998 ; Jahrgang 17, Nr. 22. S. 6730-6738.
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T1 - Competence in Bacillus subtilis is controlled by regulated proteolysis of a transcription factor

AU - Turgay, Kürşad

AU - Hahn, Jeanette

AU - Burghoorn, Jan

AU - Dubnau, David

N1 - Copyright: Copyright 2007 Elsevier B.V., All rights reserved.

PY - 1998/11/16

Y1 - 1998/11/16

N2 - Competence is a physiological state, distinct from sporulation and vegetative growth, that enables cells to bind and internalize transforming DNA. The transcriptional regulator ComK drives the development of competence in Bacillus subtilis. ComK is directly required for its own transcription as well as for the transcription of the genes that encode DNA transport proteins. When ComK is sequestered by binding to a complex of the proteins MecA and ClpC, the positive feedback loop leading to ComK synthesis is interrupted. The small protein ComS, produced as a result of signaling by a quorum-sensing two-component regulatory pathway, triggers the release of ComK from the complex, enabling comK transcription to occur. We show here, based on in vivo and in vitro experiments, that ComK accumulation is also regulated by proteolysis and that binding to MecA targets ComK for degradation by the ClpP protease in association with ClpC. The release of ComK from binding by MecA and ClpC, which occurs when ComS is synthesized, protects ComK from proteolysis. Following this release, the rates of MecA and ComS degradation by ClpCP are increased in our in vitro system. In this novel system, MecA serves to recruit ComK to the ClpCP protease and connects ComK degradation to the quorum-sensing signal-transduction pathway, thereby regulating a key developmental process. This is the first regulated degradation system in which a specific targeting molecule serves such a function.

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