TY - JOUR

T1 - Compatible solutes improve cryopreservation of human endothelial cells

AU - Sun, Huan

AU - Glasmacher, Birgit

AU - Hofmann, Nicola

N1 - This work was supported by funding from the Deutsche Forschungsgemeinschaft (DFG German Research Foundation) for the cluster of excellence REBIRTH (From Regenerative Biology to Reconstructive Therapy), EXC 62/1. We thank J. Kirkpatrick for providing the HPMEC-ST1.6R cell line.

PY - 2012

Y1 - 2012

N2 - Dimethyl sulfoxide (DMSO) is till now a widely used cryoprotective agent for cryopreservation of cells. Since high concentrations of DMSO have detrimental effects on cell functioning the aim of this study is to reduce the DMSO concentration by using compatible solutes (CS). These are small organic osmolytes that microorganisms synthesize and accumulate to counteract stress factors. Three CS, hydroxyectoine, ectoine and L-proline were investigated as cryoprotective agents for the cryopreservation of the human endothelial cell line HPMEC-ST 1.6R. They were either supplemented to freezing or to cell culture medium. L-proline was the most effective CS, the efficiency of recultivation was improved by more than 100%. A combination of L-proline and ectoine in the cell culture medium resulted in an improvement by 63%. Our results show that L-proline and ectoine could be used as additional CPAs to improve the cryopreservation of human endothelial cells in the presence of low DMSO concentration.

AB - Dimethyl sulfoxide (DMSO) is till now a widely used cryoprotective agent for cryopreservation of cells. Since high concentrations of DMSO have detrimental effects on cell functioning the aim of this study is to reduce the DMSO concentration by using compatible solutes (CS). These are small organic osmolytes that microorganisms synthesize and accumulate to counteract stress factors. Three CS, hydroxyectoine, ectoine and L-proline were investigated as cryoprotective agents for the cryopreservation of the human endothelial cell line HPMEC-ST 1.6R. They were either supplemented to freezing or to cell culture medium. L-proline was the most effective CS, the efficiency of recultivation was improved by more than 100%. A combination of L-proline and ectoine in the cell culture medium resulted in an improvement by 63%. Our results show that L-proline and ectoine could be used as additional CPAs to improve the cryopreservation of human endothelial cells in the presence of low DMSO concentration.

KW - Cryopreservation

KW - Ectoine

KW - Hydroxyectoine

KW - L-proline, compatible solutes

UR - http://www.scopus.com/inward/record.url?scp=84872560246&partnerID=8YFLogxK

M3 - Article

C2 - 23250408

AN - SCOPUS:84872560246

VL - 33

SP - 485

EP - 493

JO - Cryo-letters

JF - Cryo-letters

SN - 0143-2044

IS - 6

ER -