TY - JOUR

T1 - Comparative study of polyhydroxyalkanoates production from acidified and anaerobically treated brewery wastewater using enriched mixed microbial culture

AU - Tamang, Pravesh

AU - Banerjee, Rintu

AU - Köster, Stephan

AU - Nogueira, Regina

N1 - Funding Information: We thank Mrs. Karen Kock and Mr. Benjamin Schwanda, from the Institute for Sanitary Engineering and Waste Management (ISAH), Leibniz Universität Hannover for their technical assistance in this work. We acknowledge Dr. Ing. Corrina Lorey (ISAH) for her contribution in the GC quantification of PHA. We acknowledge the DAAD Sandwich Model Scholarships for Master's Students of the IITs and IIMs, 2016/17, who funded the research stay of Mr. Pravesh Tamang in Germany (Personal Ref No: 91635161 ). We also acknowledge the Graduiertenakademie at the Leibniz Universität Hannover for the Exposé scholarship awarded to Mr. Pravesh Tamang. We would also like to thank Indian Council of Agiricultural Research (ICAR) for granting Netaji Subhas- ICAR- International fellowship to Mr. Pravesh Tamang for further continuation of this research. Funding Information: We thank Mrs. Karen Kock and Mr. Benjamin Schwanda, from the Institute for Sanitary Engineering and Waste Management (ISAH), Leibniz Universit?t Hannover for their technical assistance in this work. We acknowledge Dr. Ing. Corrina Lorey (ISAH) for her contribution in the GC quantification of PHA. We acknowledge the DAAD Sandwich Model Scholarships for Master's Students of the IITs and IIMs, 2016/17, who funded the research stay of Mr. Pravesh Tamang in Germany (Personal Ref No: 91635161). We also acknowledge the Graduiertenakademie at the Leibniz Universit?t Hannover for the Expos? scholarship awarded to Mr. Pravesh Tamang. We would also like to thank Indian Council of Agiricultural Research (ICAR) for granting Netaji Subhas- ICAR- International fellowship to Mr. Pravesh Tamang for further continuation of this research.

PY - 2019/4

Y1 - 2019/4

N2 - The production of polyhydroxyalkanoates (PHA) from wastewaters using microbial mixed cultures (MMC) has been attracting increased interest because of PHA's biodegradability characteristics. Production of PHA by an MMC enriched with PHA-accumulating bacteria was compared using anaerobically treated and acidified brewery wastewaters under various feeding strategies, namely pulse and batch feed addition. To obtain an enriched MMC, a sequencing batch reactor was inoculated with activated sludge fed with acetate and subjected to aerobic dynamic feeding. The enriched MMC was able to accumulate PHA up to 72.6% of cell dry weight (CDW) with pulse addition of acetate controlled by the dissolved oxygen (DO) concentration in the reactor. In a batch accumulation experiment with acetate, the PHA content achieved (28.5% CDW) was less than that of the pulse feeding strategy with the same amount of acetate (~ 2000 mg C/L). Using anaerobically treated and acidified brewery wastewater fed in pulses, the maximum PHA accumulated by the enriched MMC was similar for both wastewaters (45% CDW), in spite of the higher volatile fatty acid concentration in acidified brewery wastewater. The pulse feed addition controlled by the DO concentration was difficult to implement for wastewater as compared to acetate because the difference in DO concentration between substrate availability and depletion was low. For the batch addition of acidified wastewater, a slightly lower PHA content (39% CDW) was obtained. These results show that both brewery wastewaters can be utilized for PHA production with a similar maximum PHA storage capacity.

AB - The production of polyhydroxyalkanoates (PHA) from wastewaters using microbial mixed cultures (MMC) has been attracting increased interest because of PHA's biodegradability characteristics. Production of PHA by an MMC enriched with PHA-accumulating bacteria was compared using anaerobically treated and acidified brewery wastewaters under various feeding strategies, namely pulse and batch feed addition. To obtain an enriched MMC, a sequencing batch reactor was inoculated with activated sludge fed with acetate and subjected to aerobic dynamic feeding. The enriched MMC was able to accumulate PHA up to 72.6% of cell dry weight (CDW) with pulse addition of acetate controlled by the dissolved oxygen (DO) concentration in the reactor. In a batch accumulation experiment with acetate, the PHA content achieved (28.5% CDW) was less than that of the pulse feeding strategy with the same amount of acetate (~ 2000 mg C/L). Using anaerobically treated and acidified brewery wastewater fed in pulses, the maximum PHA accumulated by the enriched MMC was similar for both wastewaters (45% CDW), in spite of the higher volatile fatty acid concentration in acidified brewery wastewater. The pulse feed addition controlled by the DO concentration was difficult to implement for wastewater as compared to acetate because the difference in DO concentration between substrate availability and depletion was low. For the batch addition of acidified wastewater, a slightly lower PHA content (39% CDW) was obtained. These results show that both brewery wastewaters can be utilized for PHA production with a similar maximum PHA storage capacity.

KW - Aerobic dynamic feeding

KW - Brewery wastewater

KW - Microbial mixed culture

KW - PHA-accumulating bacteria

KW - Polyhydroxyalkanoates

KW - Pulse feeding and batch feeding

KW - Fermentation

KW - Microbial Consortia

KW - Acetic Acid

KW - Sewage

KW - Fatty Acids, Volatile

KW - Bioreactors/microbiology

KW - Polyhydroxyalkanoates/metabolism

KW - Waste Disposal, Fluid/methods

KW - Waste Water/chemistry

UR - http://www.scopus.com/inward/record.url?scp=85055544439&partnerID=8YFLogxK

U2 - 10.1016/j.jes.2018.09.001

DO - 10.1016/j.jes.2018.09.001

M3 - Article

C2 - 30665633

AN - SCOPUS:85055544439

VL - 78

SP - 137

EP - 146

JO - Journal of Environmental Sciences (China)

JF - Journal of Environmental Sciences (China)

SN - 1001-0742

ER -