TY - JOUR

T1 - Comparative analysis of salt-induced changes in the root proteome of two accessions of the halophyte Cakile maritima

AU - Belghith, Ikram

AU - Senkler, Jennifer

AU - Hildebrandt, Tatjana

AU - Abdelly, Chedly

AU - Braun, Hans Peter

AU - Debez, Ahmed

N1 - Funding Information: This study was supported by the German Academic Exchange Service (DAAD) in the framework of the project “ Proteomics and Halophyte Stress Tolerance ” (ID 57247769 ) of the program: Transformation partnership-Line 4 and the Tunisian Ministry of Higher Education and Scientific Research . Funding Information: We thank Dagmar Lewejohann and Marianne Langer of the Institute of Leibniz Hannover for their excellent technical assistance. Publisher Copyright: © 2018 Elsevier Masson SAS Copyright: Copyright 2019 Elsevier B.V., All rights reserved.

PY - 2018/6/21

Y1 - 2018/6/21

N2 - NaCl stress is a major abiotic stress factor limiting the productivity and the geographical distribution of many plant species. Although halophytes are able to withstand and even to require salt in the rhizosphere, roots are the most sensitive organs to salinity. Here, we investigate the variability of salt tolerance in two Tunisian accessions of the halophyte Cakile maritima (Raoued and Djerba, harvested from the semi-arid and arid Mediterranean bioclimatic stages, respectively) with a special emphasis on the proteomic changes in roots. Seedlings were hydroponically grown for one month under salt-free conditions and subsequently at three salinities (0, 100, and 300 mM NaCl). Physiological parameters (plant growth, water content, Na + , K + contents) and root protein profiles were analyzed. Plant biomass was higher in Raoued than in Djerba but the latter was impacted to a lesser extent by salinity, notably due to lower sodium accumulation and higher selectivity for K + . 121 and 97 salt-responsive proteins were identified in Djerba and Raoued accessions, respectively. These proteins can be assigned to several different functional categories: protein metabolism, nucleotide metabolism, amino acid metabolism, glutathione metabolism, translation and ribosome biogenesis, carbohydrate and energy metabolism, and reactive oxygen species regulation and detoxification. The comparative proteome analysis revealed that 33 proteins were salt-responsive in both accessions, while 88 and 64 proteins were salt-responsive only in the Djerba or Raoued accessions, respectively. Our results give deeper insights into the plasticity of salt-stress response of C. maritima in its native ecosystems.

AB - NaCl stress is a major abiotic stress factor limiting the productivity and the geographical distribution of many plant species. Although halophytes are able to withstand and even to require salt in the rhizosphere, roots are the most sensitive organs to salinity. Here, we investigate the variability of salt tolerance in two Tunisian accessions of the halophyte Cakile maritima (Raoued and Djerba, harvested from the semi-arid and arid Mediterranean bioclimatic stages, respectively) with a special emphasis on the proteomic changes in roots. Seedlings were hydroponically grown for one month under salt-free conditions and subsequently at three salinities (0, 100, and 300 mM NaCl). Physiological parameters (plant growth, water content, Na + , K + contents) and root protein profiles were analyzed. Plant biomass was higher in Raoued than in Djerba but the latter was impacted to a lesser extent by salinity, notably due to lower sodium accumulation and higher selectivity for K + . 121 and 97 salt-responsive proteins were identified in Djerba and Raoued accessions, respectively. These proteins can be assigned to several different functional categories: protein metabolism, nucleotide metabolism, amino acid metabolism, glutathione metabolism, translation and ribosome biogenesis, carbohydrate and energy metabolism, and reactive oxygen species regulation and detoxification. The comparative proteome analysis revealed that 33 proteins were salt-responsive in both accessions, while 88 and 64 proteins were salt-responsive only in the Djerba or Raoued accessions, respectively. Our results give deeper insights into the plasticity of salt-stress response of C. maritima in its native ecosystems.

KW - Halophytes

KW - Proteome profiling

KW - Roots

KW - Salinity

KW - Variability

UR - http://www.scopus.com/inward/record.url?scp=85049037332&partnerID=8YFLogxK

U2 - 10.1016/j.plaphy.2018.06.029

DO - 10.1016/j.plaphy.2018.06.029

M3 - Article

C2 - 29957572

AN - SCOPUS:85049037332

VL - 130

SP - 20

EP - 29

JO - Plant physiology and biochemistry

JF - Plant physiology and biochemistry

SN - 0981-9428

ER -