Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 18-22 |
| Seitenumfang | 5 |
| Fachzeitschrift | Biochimica et Biophysica Acta - Gene Structure and Expression |
| Jahrgang | 1352 |
| Ausgabenummer | 1 |
| Publikationsstatus | Veröffentlicht - 2 Mai 1997 |
| Extern publiziert | Ja |
Abstract
The gene encoding the high potential iron-sulfur protein (HiPIP) of Chromatium vinosum strain D (DSM 180(T)) was cloned from an EcoRI-HindIII digest of genomic DNA. A nucleotide sequence of 648 bp length was determined which contained the coding region and putative promoter and termination sites. The gene codes for a 122 residue 12761 Da protein. The C-terminal 85 residues are those of the previously biochemically determined sequence, whereas the N-terminal 37 residues constitute a leader peptide which shows characteristics of the double arginine signal sequences of complex cofactor containing periplasmic proteins.
ASJC Scopus Sachgebiete
- Biochemie, Genetik und Molekularbiologie (insg.)
- Strukturelle Biologie
- Biochemie, Genetik und Molekularbiologie (insg.)
- Biophysik
- Biochemie, Genetik und Molekularbiologie (insg.)
- Biochemie
- Biochemie, Genetik und Molekularbiologie (insg.)
- Genetik
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in: Biochimica et Biophysica Acta - Gene Structure and Expression, Jahrgang 1352, Nr. 1, 02.05.1997, S. 18-22.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Cloning and sequencing of the gene encoding the high potential iron-sulfur protein (HiPIP) from the purple sulfur bacterium Chromatium vinosum
AU - Brüser, Thomas
AU - Trüper, Hans G.
AU - Dahl, Christiane
N1 - Funding Information: T.B. thanks the Cusanuswerk for a doctoral grant. This study was supported by the Fonds der Chemischen Industrie (H.G.T.). Copyright: Copyright 2007 Elsevier B.V., All rights reserved.
PY - 1997/5/2
Y1 - 1997/5/2
N2 - The gene encoding the high potential iron-sulfur protein (HiPIP) of Chromatium vinosum strain D (DSM 180(T)) was cloned from an EcoRI-HindIII digest of genomic DNA. A nucleotide sequence of 648 bp length was determined which contained the coding region and putative promoter and termination sites. The gene codes for a 122 residue 12761 Da protein. The C-terminal 85 residues are those of the previously biochemically determined sequence, whereas the N-terminal 37 residues constitute a leader peptide which shows characteristics of the double arginine signal sequences of complex cofactor containing periplasmic proteins.
AB - The gene encoding the high potential iron-sulfur protein (HiPIP) of Chromatium vinosum strain D (DSM 180(T)) was cloned from an EcoRI-HindIII digest of genomic DNA. A nucleotide sequence of 648 bp length was determined which contained the coding region and putative promoter and termination sites. The gene codes for a 122 residue 12761 Da protein. The C-terminal 85 residues are those of the previously biochemically determined sequence, whereas the N-terminal 37 residues constitute a leader peptide which shows characteristics of the double arginine signal sequences of complex cofactor containing periplasmic proteins.
KW - Chromatium vinosum
KW - gene cloning
KW - gene sequencing
UR - http://www.scopus.com/inward/record.url?scp=0042955484&partnerID=8YFLogxK
U2 - 10.1016/S0167-4781(97)00033-X
DO - 10.1016/S0167-4781(97)00033-X
M3 - Article
C2 - 9177478
AN - SCOPUS:0042955484
VL - 1352
SP - 18
EP - 22
JO - Biochimica et Biophysica Acta - Gene Structure and Expression
JF - Biochimica et Biophysica Acta - Gene Structure and Expression
SN - 0167-4781
IS - 1
ER -