Details
| Originalsprache | Englisch |
|---|---|
| Titel des Sammelwerks | Protocols for In Vitro Propagation of Ornamental Plants |
| Seiten | 281-290 |
| Seitenumfang | 10 |
| ISBN (elektronisch) | 978-1-60327-114-1 |
| Publikationsstatus | Veröffentlicht - 2009 |
Publikationsreihe
| Name | Methods in molecular biology (Clifton, N.J.) |
|---|---|
| Herausgeber (Verlag) | Humana Press |
| Band | 589 |
| ISSN (Print) | 1064-3745 |
Abstract
Cyclamen (Cyclamen persicum) is an economically important ornamental pot plant with local use as cut flower as well. Traditionally, it is propagated via seeds, but interest is given in vegetative propagation of parental lines as well as superior single plants. Somatic embryogenesis is an efficient in vitro propagation method for many cyclamen cultivars. Starting from ovules of unpollinated flowers, callus is induced and propagated in a medium containing 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-(gamma,gamma-dimethylallylamino)purine (2iP). Transfer to hormone-free medium results in the differentiation of somatic embryos, which afterwards germinate on the same medium. These first culture stages take about 6-7 months and are carried out in complete darkness. Two to four months after the transfer to light, plantlets develop which can be acclimatized in the greenhouse. The regenerated plants are characterized by low percentages of somaclonal variation. This protocol has proven useful not only for clonal propagation, but also for artificial seed preparation, cryopreservation, genetic transformation and protoplast regeneration.
ASJC Scopus Sachgebiete
- Biochemie, Genetik und Molekularbiologie (insg.)
- Molekularbiologie
- Biochemie, Genetik und Molekularbiologie (insg.)
- Genetik
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Protocols for In Vitro Propagation of Ornamental Plants. 2009. S. 281-290 (Methods in molecular biology (Clifton, N.J.); Band 589).
Publikation: Beitrag in Buch/Bericht/Sammelwerk/Konferenzband › Beitrag in Buch/Sammelwerk › Forschung › Peer-Review
}
TY - CHAP
T1 - Clonal propagation of Cyclamen persicum via somatic embryogenesis.
AU - Winkelmann, Traud
PY - 2009
Y1 - 2009
N2 - Cyclamen (Cyclamen persicum) is an economically important ornamental pot plant with local use as cut flower as well. Traditionally, it is propagated via seeds, but interest is given in vegetative propagation of parental lines as well as superior single plants. Somatic embryogenesis is an efficient in vitro propagation method for many cyclamen cultivars. Starting from ovules of unpollinated flowers, callus is induced and propagated in a medium containing 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-(gamma,gamma-dimethylallylamino)purine (2iP). Transfer to hormone-free medium results in the differentiation of somatic embryos, which afterwards germinate on the same medium. These first culture stages take about 6-7 months and are carried out in complete darkness. Two to four months after the transfer to light, plantlets develop which can be acclimatized in the greenhouse. The regenerated plants are characterized by low percentages of somaclonal variation. This protocol has proven useful not only for clonal propagation, but also for artificial seed preparation, cryopreservation, genetic transformation and protoplast regeneration.
AB - Cyclamen (Cyclamen persicum) is an economically important ornamental pot plant with local use as cut flower as well. Traditionally, it is propagated via seeds, but interest is given in vegetative propagation of parental lines as well as superior single plants. Somatic embryogenesis is an efficient in vitro propagation method for many cyclamen cultivars. Starting from ovules of unpollinated flowers, callus is induced and propagated in a medium containing 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-(gamma,gamma-dimethylallylamino)purine (2iP). Transfer to hormone-free medium results in the differentiation of somatic embryos, which afterwards germinate on the same medium. These first culture stages take about 6-7 months and are carried out in complete darkness. Two to four months after the transfer to light, plantlets develop which can be acclimatized in the greenhouse. The regenerated plants are characterized by low percentages of somaclonal variation. This protocol has proven useful not only for clonal propagation, but also for artificial seed preparation, cryopreservation, genetic transformation and protoplast regeneration.
UR - http://www.scopus.com/inward/record.url?scp=77449097115&partnerID=8YFLogxK
U2 - 10.1007/978-1-60327-114-1_26
DO - 10.1007/978-1-60327-114-1_26
M3 - Contribution to book/anthology
C2 - 20099110
AN - SCOPUS:77449097115
SN - 978-1-60327-390-9
SN - 978-1-61779-673-9
T3 - Methods in molecular biology (Clifton, N.J.)
SP - 281
EP - 290
BT - Protocols for In Vitro Propagation of Ornamental Plants
ER -