Clay Types Modulate the Toxicity of Low Concentrated Copper Oxide Nanoparticles Toward Springtails in Artificial Test Soils

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Autoren

  • Jonas Fischer
  • Ghanem D.A. Talal
  • Laura S. Schnee
  • Patricks V. Otomo
  • Juliane Filser

Organisationseinheiten

Externe Organisationen

  • Universität Bremen
  • University of The Free State
Forschungs-netzwerk anzeigen

Details

OriginalspracheEnglisch
Seiten (von - bis)2454-2465
Seitenumfang12
FachzeitschriftEnvironmental Toxicology and Chemistry
Jahrgang41
Ausgabenummer10
Frühes Online-Datum20 Juli 2022
PublikationsstatusVeröffentlicht - Okt. 2022

Abstract

Copper oxide nanoparticles (CuO-NPs) can be applied as an efficient alternative to conventional Cu in agriculture. Negative effects of CuO-NPs on soil organisms were found, but only in clay-rich loamy soils. It is hypothesized that clay–NP interactions are the origin of the observed toxic effects. In the present study, artificial Organisation for Economic Co-operation and Development soils containing 30% of kaolin or montmorillonite as clay type were spiked with 1–32 mg Cu/kg of uncoated CuO-NPs or CuCl2. We performed 28-day reproduction tests with springtails of the species Folsomia candida and recorded the survival, reproduction, dry weight, and Cu content of adults. In a second experiment, molting frequency and the Cu content of exuviae, as well as the biochemical endpoints metallothionein and catalase (CAT) in springtails, were investigated. In the reproduction assay, negative effects on all endpoints were observed, but only in soils containing montmorillonite and mostly for CuO-NPs. For the biochemical endpoints and Cu content of exuviae, effects were clearly distinct between Cu forms in montmorillonite soil, but a significant reduction compared to the control was only found for CAT activity. Therefore, the reduced CAT activity in CuO-NP-montmorillonite soil might be responsible for the observed toxicity, potentially resulting from reactive oxygen species formation overloading the antioxidant system. This process seems to be highly concentration-dependent, because all endpoints investigated in reproduction and biochemical assays of CuO-NP-montmorillonite treatments showed a nonlinear dose–response relationship and were constantly reduced by approximately 40% at a field-realistic concentration of 3 mg/kg, but not at 32 mg/kg. The results underline that clay–CuO-NP interactions are crucial for their toxic behavior, especially at low, field-realistic concentrations, which should be considered for risk assessment of CuO-NPs. Environ Toxicol Chem 2022;41:2454–2465.

ASJC Scopus Sachgebiete

Zitieren

Clay Types Modulate the Toxicity of Low Concentrated Copper Oxide Nanoparticles Toward Springtails in Artificial Test Soils. / Fischer, Jonas; Talal, Ghanem D.A.; Schnee, Laura S. et al.
in: Environmental Toxicology and Chemistry, Jahrgang 41, Nr. 10, 10.2022, S. 2454-2465.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Fischer J, Talal GDA, Schnee LS, Otomo PV, Filser J. Clay Types Modulate the Toxicity of Low Concentrated Copper Oxide Nanoparticles Toward Springtails in Artificial Test Soils. Environmental Toxicology and Chemistry. 2022 Okt;41(10):2454-2465. Epub 2022 Jul 20. doi: 10.1002/etc.5440
Fischer, Jonas ; Talal, Ghanem D.A. ; Schnee, Laura S. et al. / Clay Types Modulate the Toxicity of Low Concentrated Copper Oxide Nanoparticles Toward Springtails in Artificial Test Soils. in: Environmental Toxicology and Chemistry. 2022 ; Jahrgang 41, Nr. 10. S. 2454-2465.
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title = "Clay Types Modulate the Toxicity of Low Concentrated Copper Oxide Nanoparticles Toward Springtails in Artificial Test Soils",
abstract = "Copper oxide nanoparticles (CuO-NPs) can be applied as an efficient alternative to conventional Cu in agriculture. Negative effects of CuO-NPs on soil organisms were found, but only in clay-rich loamy soils. It is hypothesized that clay–NP interactions are the origin of the observed toxic effects. In the present study, artificial Organisation for Economic Co-operation and Development soils containing 30% of kaolin or montmorillonite as clay type were spiked with 1–32 mg Cu/kg of uncoated CuO-NPs or CuCl2. We performed 28-day reproduction tests with springtails of the species Folsomia candida and recorded the survival, reproduction, dry weight, and Cu content of adults. In a second experiment, molting frequency and the Cu content of exuviae, as well as the biochemical endpoints metallothionein and catalase (CAT) in springtails, were investigated. In the reproduction assay, negative effects on all endpoints were observed, but only in soils containing montmorillonite and mostly for CuO-NPs. For the biochemical endpoints and Cu content of exuviae, effects were clearly distinct between Cu forms in montmorillonite soil, but a significant reduction compared to the control was only found for CAT activity. Therefore, the reduced CAT activity in CuO-NP-montmorillonite soil might be responsible for the observed toxicity, potentially resulting from reactive oxygen species formation overloading the antioxidant system. This process seems to be highly concentration-dependent, because all endpoints investigated in reproduction and biochemical assays of CuO-NP-montmorillonite treatments showed a nonlinear dose–response relationship and were constantly reduced by approximately 40% at a field-realistic concentration of 3 mg/kg, but not at 32 mg/kg. The results underline that clay–CuO-NP interactions are crucial for their toxic behavior, especially at low, field-realistic concentrations, which should be considered for risk assessment of CuO-NPs. Environ Toxicol Chem 2022;41:2454–2465.",
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T1 - Clay Types Modulate the Toxicity of Low Concentrated Copper Oxide Nanoparticles Toward Springtails in Artificial Test Soils

AU - Fischer, Jonas

AU - Talal, Ghanem D.A.

AU - Schnee, Laura S.

AU - Otomo, Patricks V.

AU - Filser, Juliane

N1 - Funding Information: We would like to thank M. Koelling and S. Pape from MARUM Bremen for X‐ray fluorescence measurements. From the University of Bremen, we would like to thank the following colleagues: V. Koch for Cu pore water measurements, J. Rosenau for providing biomarker materials, C. Vogt for X‐ray diffraction measurements and A. Rother for support in AAS measurements. The first author was funded by a PhD scholarship from Hans‐Böckler‐Stiftung within the framework of the graduate school NanoCompetence (PK041). Open Access funding enabled and organized by Projekt DEAL.

PY - 2022/10

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N2 - Copper oxide nanoparticles (CuO-NPs) can be applied as an efficient alternative to conventional Cu in agriculture. Negative effects of CuO-NPs on soil organisms were found, but only in clay-rich loamy soils. It is hypothesized that clay–NP interactions are the origin of the observed toxic effects. In the present study, artificial Organisation for Economic Co-operation and Development soils containing 30% of kaolin or montmorillonite as clay type were spiked with 1–32 mg Cu/kg of uncoated CuO-NPs or CuCl2. We performed 28-day reproduction tests with springtails of the species Folsomia candida and recorded the survival, reproduction, dry weight, and Cu content of adults. In a second experiment, molting frequency and the Cu content of exuviae, as well as the biochemical endpoints metallothionein and catalase (CAT) in springtails, were investigated. In the reproduction assay, negative effects on all endpoints were observed, but only in soils containing montmorillonite and mostly for CuO-NPs. For the biochemical endpoints and Cu content of exuviae, effects were clearly distinct between Cu forms in montmorillonite soil, but a significant reduction compared to the control was only found for CAT activity. Therefore, the reduced CAT activity in CuO-NP-montmorillonite soil might be responsible for the observed toxicity, potentially resulting from reactive oxygen species formation overloading the antioxidant system. This process seems to be highly concentration-dependent, because all endpoints investigated in reproduction and biochemical assays of CuO-NP-montmorillonite treatments showed a nonlinear dose–response relationship and were constantly reduced by approximately 40% at a field-realistic concentration of 3 mg/kg, but not at 32 mg/kg. The results underline that clay–CuO-NP interactions are crucial for their toxic behavior, especially at low, field-realistic concentrations, which should be considered for risk assessment of CuO-NPs. Environ Toxicol Chem 2022;41:2454–2465.

AB - Copper oxide nanoparticles (CuO-NPs) can be applied as an efficient alternative to conventional Cu in agriculture. Negative effects of CuO-NPs on soil organisms were found, but only in clay-rich loamy soils. It is hypothesized that clay–NP interactions are the origin of the observed toxic effects. In the present study, artificial Organisation for Economic Co-operation and Development soils containing 30% of kaolin or montmorillonite as clay type were spiked with 1–32 mg Cu/kg of uncoated CuO-NPs or CuCl2. We performed 28-day reproduction tests with springtails of the species Folsomia candida and recorded the survival, reproduction, dry weight, and Cu content of adults. In a second experiment, molting frequency and the Cu content of exuviae, as well as the biochemical endpoints metallothionein and catalase (CAT) in springtails, were investigated. In the reproduction assay, negative effects on all endpoints were observed, but only in soils containing montmorillonite and mostly for CuO-NPs. For the biochemical endpoints and Cu content of exuviae, effects were clearly distinct between Cu forms in montmorillonite soil, but a significant reduction compared to the control was only found for CAT activity. Therefore, the reduced CAT activity in CuO-NP-montmorillonite soil might be responsible for the observed toxicity, potentially resulting from reactive oxygen species formation overloading the antioxidant system. This process seems to be highly concentration-dependent, because all endpoints investigated in reproduction and biochemical assays of CuO-NP-montmorillonite treatments showed a nonlinear dose–response relationship and were constantly reduced by approximately 40% at a field-realistic concentration of 3 mg/kg, but not at 32 mg/kg. The results underline that clay–CuO-NP interactions are crucial for their toxic behavior, especially at low, field-realistic concentrations, which should be considered for risk assessment of CuO-NPs. Environ Toxicol Chem 2022;41:2454–2465.

KW - Biomarkers

KW - Copper

KW - Nanoparticles

KW - Soil ecotoxicology

KW - Soil invertebrates

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JF - Environmental Toxicology and Chemistry

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