Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 747-760 |
| Seitenumfang | 14 |
| Fachzeitschrift | FEMS microbiology ecology |
| Jahrgang | 90 |
| Ausgabenummer | 3 |
| Publikationsstatus | Veröffentlicht - 1 Dez. 2014 |
Abstract
Novel insights are provided regarding aerobic chemolithotrophic growth of Thermocrinis ruber OC14/7/2 on the electron donors arsenite and monothioarsenate. Thermocrinis ruber is a hyperthermophilic bacterium that thrives in pH-neutral to alkaline hot springs and grows on hydrogen, elemental sulfur, and thiosulfate. Our study showed that T. ruber can also utilize arsenite as sole electron donor producing arsenate. Growth rates of 0.024 h -1 were lower than for oxidation of thiosulfate to sulfate (μ = 0.247 h -1). Fast growth was observed on monothioarsenate (μ = 0.359 h -1), comprising different abiotic and biotic redox interactions. The initial dominant process was abiotic transformation of monothioarsenate to arsenate and elemental sulfur, followed by microbial oxidation of sulfur to sulfate. Elevated microbial activity during stationary growth of T. ruber might be explained by microbial oxidation of thiosulfate and arsenite, both also products of abiotic monothioarsenate transformation. However, the observed rapid decrease of monothioarsenate, exceeding concentrations in equilibrium with its products, also indicates direct microbial oxidation of arsenic-bond S(-II) to sulfate. Free sulfide was oxidized abiotically too fast to play a role as electron donor for T. ruber. Our present laboratory and previous field studies suggest that thioarsenates can either indirectly or directly be used by (hyper)thermophiles in arsenic-sulfidic environments.
ASJC Scopus Sachgebiete
- Immunologie und Mikrobiologie (insg.)
- Mikrobiologie
- Umweltwissenschaften (insg.)
- Ökologie
- Immunologie und Mikrobiologie (insg.)
- Angewandte Mikrobiologie und Biotechnologie
Zitieren
- Standard
- Harvard
- Apa
- Vancouver
- BibTex
- RIS
in: FEMS microbiology ecology, Jahrgang 90, Nr. 3, 01.12.2014, S. 747-760.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Chemolithotrophic growth of the aerobic hyperthermophilic bacterium Thermocrinis ruber OC 14/7/2 on monothioarsenate and arsenite
AU - Haertig, Cornelia
AU - Lohmayer, Regina
AU - Kolb, Steffen
AU - Horn, Marcus A.
AU - Inskeep, William P.
AU - Planer-Friedrich, Britta
N1 - Publisher Copyright: © 2014 Federation of European Microbiological Societies. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2014/12/1
Y1 - 2014/12/1
N2 - Novel insights are provided regarding aerobic chemolithotrophic growth of Thermocrinis ruber OC14/7/2 on the electron donors arsenite and monothioarsenate. Thermocrinis ruber is a hyperthermophilic bacterium that thrives in pH-neutral to alkaline hot springs and grows on hydrogen, elemental sulfur, and thiosulfate. Our study showed that T. ruber can also utilize arsenite as sole electron donor producing arsenate. Growth rates of 0.024 h -1 were lower than for oxidation of thiosulfate to sulfate (μ = 0.247 h -1). Fast growth was observed on monothioarsenate (μ = 0.359 h -1), comprising different abiotic and biotic redox interactions. The initial dominant process was abiotic transformation of monothioarsenate to arsenate and elemental sulfur, followed by microbial oxidation of sulfur to sulfate. Elevated microbial activity during stationary growth of T. ruber might be explained by microbial oxidation of thiosulfate and arsenite, both also products of abiotic monothioarsenate transformation. However, the observed rapid decrease of monothioarsenate, exceeding concentrations in equilibrium with its products, also indicates direct microbial oxidation of arsenic-bond S(-II) to sulfate. Free sulfide was oxidized abiotically too fast to play a role as electron donor for T. ruber. Our present laboratory and previous field studies suggest that thioarsenates can either indirectly or directly be used by (hyper)thermophiles in arsenic-sulfidic environments.
AB - Novel insights are provided regarding aerobic chemolithotrophic growth of Thermocrinis ruber OC14/7/2 on the electron donors arsenite and monothioarsenate. Thermocrinis ruber is a hyperthermophilic bacterium that thrives in pH-neutral to alkaline hot springs and grows on hydrogen, elemental sulfur, and thiosulfate. Our study showed that T. ruber can also utilize arsenite as sole electron donor producing arsenate. Growth rates of 0.024 h -1 were lower than for oxidation of thiosulfate to sulfate (μ = 0.247 h -1). Fast growth was observed on monothioarsenate (μ = 0.359 h -1), comprising different abiotic and biotic redox interactions. The initial dominant process was abiotic transformation of monothioarsenate to arsenate and elemental sulfur, followed by microbial oxidation of sulfur to sulfate. Elevated microbial activity during stationary growth of T. ruber might be explained by microbial oxidation of thiosulfate and arsenite, both also products of abiotic monothioarsenate transformation. However, the observed rapid decrease of monothioarsenate, exceeding concentrations in equilibrium with its products, also indicates direct microbial oxidation of arsenic-bond S(-II) to sulfate. Free sulfide was oxidized abiotically too fast to play a role as electron donor for T. ruber. Our present laboratory and previous field studies suggest that thioarsenates can either indirectly or directly be used by (hyper)thermophiles in arsenic-sulfidic environments.
KW - Aquificales
KW - Biogeochemistry
KW - Chemolithotrophy
KW - Thioarsenic species
KW - Yellowstone National Park
UR - http://www.scopus.com/inward/record.url?scp=84914816148&partnerID=8YFLogxK
U2 - 10.1111/1574-6941.12431
DO - 10.1111/1574-6941.12431
M3 - Article
VL - 90
SP - 747
EP - 760
JO - FEMS microbiology ecology
JF - FEMS microbiology ecology
SN - 0168-6496
IS - 3
ER -