Details
| Originalsprache | Englisch |
|---|---|
| Aufsatznummer | 102554 |
| Seitenumfang | 8 |
| Fachzeitschrift | Current opinion in plant biology |
| Jahrgang | 80 |
| Frühes Online-Datum | 30 Mai 2024 |
| Publikationsstatus | Veröffentlicht - Aug. 2024 |
Abstract
Elucidating the biochemical pathways of specialised metabolites in plants is key to enable or improve their sustainable biotechnological production. Chemical tools can greatly facilitate the discovery of biosynthetic genes and enzymes. Here, we summarise transdisciplinary approaches where methods from chemistry and chemical biology helped to overcome key challenges of pathway elucidation. Based on recent examples, we describe how state-of-the-art isotope labelling experiments can guide the selection of biosynthetic gene candidates, how affinity-based probes enable the identification of novel enzymes, how semisynthesis can improve the availability of elusive pathway intermediates, and how biomimetic reactions provide a better understanding of inherent chemical reactivity. We anticipate that a wider application of such chemical methods will accelerate the pace of pathway elucidation in plants.
ASJC Scopus Sachgebiete
- Agrar- und Biowissenschaften (insg.)
- Pflanzenkunde
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in: Current opinion in plant biology, Jahrgang 80, 102554, 08.2024.
Publikation: Beitrag in Fachzeitschrift › Übersichtsarbeit › Forschung › Peer-Review
}
TY - JOUR
T1 - Chemical tools for unpicking plant specialised metabolic pathways
AU - Seligmann, Benedikt
AU - Liu, Shenyu
AU - Franke, Jakob
N1 - Publisher Copyright: © 2024 The Authors
PY - 2024/8
Y1 - 2024/8
N2 - Elucidating the biochemical pathways of specialised metabolites in plants is key to enable or improve their sustainable biotechnological production. Chemical tools can greatly facilitate the discovery of biosynthetic genes and enzymes. Here, we summarise transdisciplinary approaches where methods from chemistry and chemical biology helped to overcome key challenges of pathway elucidation. Based on recent examples, we describe how state-of-the-art isotope labelling experiments can guide the selection of biosynthetic gene candidates, how affinity-based probes enable the identification of novel enzymes, how semisynthesis can improve the availability of elusive pathway intermediates, and how biomimetic reactions provide a better understanding of inherent chemical reactivity. We anticipate that a wider application of such chemical methods will accelerate the pace of pathway elucidation in plants.
AB - Elucidating the biochemical pathways of specialised metabolites in plants is key to enable or improve their sustainable biotechnological production. Chemical tools can greatly facilitate the discovery of biosynthetic genes and enzymes. Here, we summarise transdisciplinary approaches where methods from chemistry and chemical biology helped to overcome key challenges of pathway elucidation. Based on recent examples, we describe how state-of-the-art isotope labelling experiments can guide the selection of biosynthetic gene candidates, how affinity-based probes enable the identification of novel enzymes, how semisynthesis can improve the availability of elusive pathway intermediates, and how biomimetic reactions provide a better understanding of inherent chemical reactivity. We anticipate that a wider application of such chemical methods will accelerate the pace of pathway elucidation in plants.
UR - http://www.scopus.com/inward/record.url?scp=85194418649&partnerID=8YFLogxK
U2 - 10.1016/j.pbi.2024.102554
DO - 10.1016/j.pbi.2024.102554
M3 - Review article
AN - SCOPUS:85194418649
VL - 80
JO - Current opinion in plant biology
JF - Current opinion in plant biology
SN - 1369-5266
M1 - 102554
ER -