Details
| Originalsprache | Englisch |
|---|---|
| Aufsatznummer | 27 |
| Seiten (von - bis) | 1-16 |
| Seitenumfang | 16 |
| Fachzeitschrift | Gels |
| Jahrgang | 6 |
| Ausgabenummer | 3 |
| Publikationsstatus | Veröffentlicht - 3 Sept. 2020 |
Abstract
Scaffolds constitute an important element in vascularized tissues and are therefore investigated for providing the desired mechanical stability and enabling vasculogenesis and angiogenesis. In this study, supplementation of hydrogels containing either Matrigel™ and rat tail collagen I (Matrigel™/rCOL) or human collagen (hCOL) with SeaPlaque™ agarose were analyzed with regard to construct thickness and formation and characteristics of endothelial cell (EC) networks compared to constructs without agarose. Additionally, the effect of increased rCOL content in Matrigel™/rCOL constructs was studied. An increase of rCOL content from 1 mg/mL to 3 mg/mL resulted in an increase of construct thickness by approximately 160%. The high rCOL content, however, impaired the formation of an EC network. The supplementation of Matrigel™/rCOL with agarose increased the thickness of the hydrogel construct by approximately 100% while supporting the formation of a stable EC network. The use of hCOL/agarose composite hydrogels led to a slight increase in the thickness of the 3D hydrogel construct and supported the formation of a multi-layered EC network compared to control constructs. Our findings suggest that agarose/collagen-based composite hydrogels are promising candidates for tissue engineering of vascularized constructs as cell viability is maintained and the formation of a stable and multi-layered EC network is supported.
ASJC Scopus Sachgebiete
- Chemische Verfahrenstechnik (insg.)
- Bioengineering
- Werkstoffwissenschaften (insg.)
- Biomaterialien
- Chemie (insg.)
- Organische Chemie
- Werkstoffwissenschaften (insg.)
- Polymere und Kunststoffe
Zitieren
- Standard
- Harvard
- Apa
- Vancouver
- BibTex
- RIS
in: Gels, Jahrgang 6, Nr. 3, 27, 03.09.2020, S. 1-16.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Characterization of tissue engineered endothelial cell networks in composite collagen-agarose hydrogels
AU - Ichanti, Houda
AU - Sladic, Sanja
AU - Kalies, Stefan
AU - Haverich, Axel
AU - Andrée, Birgit
AU - Hilfiker, Andres
N1 - Funding Information: Funding: This work was financed by the CORTISS foundation, the Deutsche Forschungsgemeinschaft (Project HA 13 06/9-1), the BMBF Project “AUREKA” and DFG Cluster of Excellence “REBIRTH”.
PY - 2020/9/3
Y1 - 2020/9/3
N2 - Scaffolds constitute an important element in vascularized tissues and are therefore investigated for providing the desired mechanical stability and enabling vasculogenesis and angiogenesis. In this study, supplementation of hydrogels containing either Matrigel™ and rat tail collagen I (Matrigel™/rCOL) or human collagen (hCOL) with SeaPlaque™ agarose were analyzed with regard to construct thickness and formation and characteristics of endothelial cell (EC) networks compared to constructs without agarose. Additionally, the effect of increased rCOL content in Matrigel™/rCOL constructs was studied. An increase of rCOL content from 1 mg/mL to 3 mg/mL resulted in an increase of construct thickness by approximately 160%. The high rCOL content, however, impaired the formation of an EC network. The supplementation of Matrigel™/rCOL with agarose increased the thickness of the hydrogel construct by approximately 100% while supporting the formation of a stable EC network. The use of hCOL/agarose composite hydrogels led to a slight increase in the thickness of the 3D hydrogel construct and supported the formation of a multi-layered EC network compared to control constructs. Our findings suggest that agarose/collagen-based composite hydrogels are promising candidates for tissue engineering of vascularized constructs as cell viability is maintained and the formation of a stable and multi-layered EC network is supported.
AB - Scaffolds constitute an important element in vascularized tissues and are therefore investigated for providing the desired mechanical stability and enabling vasculogenesis and angiogenesis. In this study, supplementation of hydrogels containing either Matrigel™ and rat tail collagen I (Matrigel™/rCOL) or human collagen (hCOL) with SeaPlaque™ agarose were analyzed with regard to construct thickness and formation and characteristics of endothelial cell (EC) networks compared to constructs without agarose. Additionally, the effect of increased rCOL content in Matrigel™/rCOL constructs was studied. An increase of rCOL content from 1 mg/mL to 3 mg/mL resulted in an increase of construct thickness by approximately 160%. The high rCOL content, however, impaired the formation of an EC network. The supplementation of Matrigel™/rCOL with agarose increased the thickness of the hydrogel construct by approximately 100% while supporting the formation of a stable EC network. The use of hCOL/agarose composite hydrogels led to a slight increase in the thickness of the 3D hydrogel construct and supported the formation of a multi-layered EC network compared to control constructs. Our findings suggest that agarose/collagen-based composite hydrogels are promising candidates for tissue engineering of vascularized constructs as cell viability is maintained and the formation of a stable and multi-layered EC network is supported.
KW - Collagen hydrogel
KW - Endothelial cell network
KW - Hybrid hydrogel
KW - Tissue engineering
UR - http://www.scopus.com/inward/record.url?scp=85092521029&partnerID=8YFLogxK
U2 - 10.3390/gels6030027
DO - 10.3390/gels6030027
M3 - Article
AN - SCOPUS:85092521029
VL - 6
SP - 1
EP - 16
JO - Gels
JF - Gels
IS - 3
M1 - 27
ER -