Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 363-373 |
| Seitenumfang | 11 |
| Fachzeitschrift | Molecular genetics and genomics |
| Jahrgang | 266 |
| Ausgabenummer | 3 |
| Publikationsstatus | Veröffentlicht - 14 Nov. 2001 |
Abstract
Two chlorophyll-deficient mutants of Chlamydomonas reinhardtii, chl1 and brs-1, are light sensitive and, when grown heterotrophically in the dark, accumulate protoporphyrin IX and exhibit yellow/orange pigmentation. The lesions in both mutants were mapped to the gene (CHLH) for the plastid-localized H subunit of the heterotrimeric magnesium chelatase that catalyzes the insertion of magnesium into protoporphyrin IX. The genetic defects in the mutants could be assigned to + 1 frameshift mutations in exon 9 (chl1) and exon 10 (brs-1) of the CHLH gene. In both mutants, the H subunit of magnesium chelatase was undetectable, but, as shown for chl1, the steady-state levels of the I and D subunits were unaltered in comparison to wild type. The CHLH gene exhibits marked light inducibility: levels of both the mRNA and the protein product are strongly increased when cultures are shifted from from the dark into the light, suggesting that this protein may play a crucial role in the light regulation of chlorophyll biosynthesis.
ASJC Scopus Sachgebiete
- Biochemie, Genetik und Molekularbiologie (insg.)
- Molekularbiologie
- Biochemie, Genetik und Molekularbiologie (insg.)
- Genetik
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in: Molecular genetics and genomics, Jahrgang 266, Nr. 3, 14.11.2001, S. 363-373.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Characterization of Chlamydomonas mutants defective in the H subunit of Mg-chelatase
AU - Chekounova, E.
AU - Voronetskaya, V.
AU - Papenbrock, J.
AU - Grimm, B.
AU - Beck, C.
N1 - Funding information: The project was supported by DFG Grants BE903/11-1 to C.F.B. and TP B15 (SFB 363) to B.G. E. Cheku-nova was the recipient of an EMBO Short-Term Fellowship and also acknowledges the support of the DFG (436RUS17/75/99).
PY - 2001/11/14
Y1 - 2001/11/14
N2 - Two chlorophyll-deficient mutants of Chlamydomonas reinhardtii, chl1 and brs-1, are light sensitive and, when grown heterotrophically in the dark, accumulate protoporphyrin IX and exhibit yellow/orange pigmentation. The lesions in both mutants were mapped to the gene (CHLH) for the plastid-localized H subunit of the heterotrimeric magnesium chelatase that catalyzes the insertion of magnesium into protoporphyrin IX. The genetic defects in the mutants could be assigned to + 1 frameshift mutations in exon 9 (chl1) and exon 10 (brs-1) of the CHLH gene. In both mutants, the H subunit of magnesium chelatase was undetectable, but, as shown for chl1, the steady-state levels of the I and D subunits were unaltered in comparison to wild type. The CHLH gene exhibits marked light inducibility: levels of both the mRNA and the protein product are strongly increased when cultures are shifted from from the dark into the light, suggesting that this protein may play a crucial role in the light regulation of chlorophyll biosynthesis.
AB - Two chlorophyll-deficient mutants of Chlamydomonas reinhardtii, chl1 and brs-1, are light sensitive and, when grown heterotrophically in the dark, accumulate protoporphyrin IX and exhibit yellow/orange pigmentation. The lesions in both mutants were mapped to the gene (CHLH) for the plastid-localized H subunit of the heterotrimeric magnesium chelatase that catalyzes the insertion of magnesium into protoporphyrin IX. The genetic defects in the mutants could be assigned to + 1 frameshift mutations in exon 9 (chl1) and exon 10 (brs-1) of the CHLH gene. In both mutants, the H subunit of magnesium chelatase was undetectable, but, as shown for chl1, the steady-state levels of the I and D subunits were unaltered in comparison to wild type. The CHLH gene exhibits marked light inducibility: levels of both the mRNA and the protein product are strongly increased when cultures are shifted from from the dark into the light, suggesting that this protein may play a crucial role in the light regulation of chlorophyll biosynthesis.
KW - Chlamydomonas
KW - CHLH gene
KW - Mg-chelatase
KW - Mutants
KW - Protoporphyrin IX
UR - http://www.scopus.com/inward/record.url?scp=0034753673&partnerID=8YFLogxK
U2 - 10.1007/s004380100574
DO - 10.1007/s004380100574
M3 - Article
C2 - 11713666
AN - SCOPUS:0034753673
VL - 266
SP - 363
EP - 373
JO - Molecular genetics and genomics
JF - Molecular genetics and genomics
SN - 1617-4615
IS - 3
ER -