Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 195-206 |
| Seitenumfang | 12 |
| Fachzeitschrift | Plant Cell, Tissue and Organ Culture |
| Jahrgang | 127 |
| Ausgabenummer | 1 |
| Frühes Online-Datum | 12 Juli 2016 |
| Publikationsstatus | Veröffentlicht - Okt. 2016 |
Abstract
Climate change and the increasing world population will lead to an increased water shortage. This gives rise to the need for plant cultivars which are drought tolerant. Solanum tuberosum L. is important not only as a nutritive rich food, but starch potatoes are of great value for the industry. Two starch genotypes of S. tuberosum L. divergently responding to osmotic stress were subjected to medium containing 0.2 M sorbitol in vitro. A targeted metabolomics approach was performed in which 42 metabolites were analysed 11 days after the transfer of the plants to the experimental medium. The sensitive genotype displayed stress responses comprising higher abundant metabolites such as phenylalanine, proline and sucrose and a decrease e.g. in GABA and fumaric acid. These can be used for protein build up, nitrogen storage and the protection through osmotic active compounds. In contrast, the tolerant genotype showed a higher abundance in compounds used as osmolytes (citric acid and proline), which might give rise to acclimatisation to the stress. Interestingly, in chromatograms of both genotypes a high sorbitol peak was detected, whereas control plants or plants treated with 4.8 % PEG 8000 did not accumulate this substance. Conclusively, sorbitol is taken up during in vitro growth, which raises the question for the fate and effect of the incorporated sorbitol.
ASJC Scopus Sachgebiete
- Agrar- und Biowissenschaften (insg.)
- Gartenbau
Ziele für nachhaltige Entwicklung
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in: Plant Cell, Tissue and Organ Culture, Jahrgang 127, Nr. 1, 10.2016, S. 195-206.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Changed composition of metabolites in Solanum tuberosum subjected to osmotic stress in vitro
T2 - Is sorbitol taken up?
AU - Bündig, C.
AU - Blume, C.
AU - Peterhänsel, C.
AU - Winkelmann, T.
PY - 2016/10
Y1 - 2016/10
N2 - Climate change and the increasing world population will lead to an increased water shortage. This gives rise to the need for plant cultivars which are drought tolerant. Solanum tuberosum L. is important not only as a nutritive rich food, but starch potatoes are of great value for the industry. Two starch genotypes of S. tuberosum L. divergently responding to osmotic stress were subjected to medium containing 0.2 M sorbitol in vitro. A targeted metabolomics approach was performed in which 42 metabolites were analysed 11 days after the transfer of the plants to the experimental medium. The sensitive genotype displayed stress responses comprising higher abundant metabolites such as phenylalanine, proline and sucrose and a decrease e.g. in GABA and fumaric acid. These can be used for protein build up, nitrogen storage and the protection through osmotic active compounds. In contrast, the tolerant genotype showed a higher abundance in compounds used as osmolytes (citric acid and proline), which might give rise to acclimatisation to the stress. Interestingly, in chromatograms of both genotypes a high sorbitol peak was detected, whereas control plants or plants treated with 4.8 % PEG 8000 did not accumulate this substance. Conclusively, sorbitol is taken up during in vitro growth, which raises the question for the fate and effect of the incorporated sorbitol.
AB - Climate change and the increasing world population will lead to an increased water shortage. This gives rise to the need for plant cultivars which are drought tolerant. Solanum tuberosum L. is important not only as a nutritive rich food, but starch potatoes are of great value for the industry. Two starch genotypes of S. tuberosum L. divergently responding to osmotic stress were subjected to medium containing 0.2 M sorbitol in vitro. A targeted metabolomics approach was performed in which 42 metabolites were analysed 11 days after the transfer of the plants to the experimental medium. The sensitive genotype displayed stress responses comprising higher abundant metabolites such as phenylalanine, proline and sucrose and a decrease e.g. in GABA and fumaric acid. These can be used for protein build up, nitrogen storage and the protection through osmotic active compounds. In contrast, the tolerant genotype showed a higher abundance in compounds used as osmolytes (citric acid and proline), which might give rise to acclimatisation to the stress. Interestingly, in chromatograms of both genotypes a high sorbitol peak was detected, whereas control plants or plants treated with 4.8 % PEG 8000 did not accumulate this substance. Conclusively, sorbitol is taken up during in vitro growth, which raises the question for the fate and effect of the incorporated sorbitol.
KW - Abiotic stress
KW - In vitro
KW - Proline
KW - Solanum tuberosum
KW - Sorbitol
KW - Starch potato
KW - Water deficiency
UR - http://www.scopus.com/inward/record.url?scp=84978121137&partnerID=8YFLogxK
U2 - 10.1007/s11240-016-1042-1
DO - 10.1007/s11240-016-1042-1
M3 - Article
VL - 127
SP - 195
EP - 206
JO - Plant Cell, Tissue and Organ Culture
JF - Plant Cell, Tissue and Organ Culture
SN - 0167-6857
IS - 1
ER -