TY - JOUR

T1 - Cell Type-Specific Adhesion and Migration on Laser-Structured Opaque Surfaces

AU - Schaeske, Jörn

AU - Fadeeva, Elena

AU - Schlie-Wolter, Sabrina

AU - Deiwick, Andrea

AU - Chichkov, Boris N.

AU - Ingendoh-Tsakmakidis, Alexandra

AU - Stiesch, Meike

AU - Winkel, Andreas

N1 - Funding Information: Acknowledgments: We would like to thank the research association BIOFABRICATION for NIFE, sponsored by the government of Lower Saxony, and the Volkswagenstiftung, for funding this project. We would also like to thank the Research Workshop of the Medical School Hannover for the construction of the frames for the optimized migration assay and Annike Brune for the technical illustration regarding the migration assay in the material and methods section. Funding Information: Niedersächsisches Ministerium für Wissenschaft und Kultur together with Volkswagen Foundation: WVZN2860.

PY - 2020/11/10

Y1 - 2020/11/10

N2 - Cytocompatibility is essential for implant approval. However, initial in vitro screenings mainly include the quantity of adherent immortalized cells and cytotoxicity. Other vital parameters, such as cell migration and an in-depth understanding of the interaction between native tissue cells and implant surfaces, are rarely considered. We investigated different laser-fabricated spike structures using primary and immortalized cell lines of fibroblasts and osteoblasts and included quantification of the cell area, aspect ratio, and focal adhesions. Furthermore, we examined the three-dimensional cell interactions with spike topographies and developed a tailored migration assay for long-term monitoring on opaque materials. While fibroblasts and osteoblasts on small spikes retained their normal morphology, cells on medium and large spikes sank into the structures, affecting the composition of the cytoskeleton and thereby changing cell shape. Up to 14 days, migration appeared stronger on small spikes, probably as a consequence of adequate focal adhesion formation and an intact cytoskeleton, whereas human primary cells revealed differences in comparison to immortalized cell lines. The use of primary cells, analysis of the cell–implant structure interaction as well as cell migration might strengthen the evaluation of cytocompatibility and thereby improve the validity regarding the putative in vivo performance of implant material.

AB - Cytocompatibility is essential for implant approval. However, initial in vitro screenings mainly include the quantity of adherent immortalized cells and cytotoxicity. Other vital parameters, such as cell migration and an in-depth understanding of the interaction between native tissue cells and implant surfaces, are rarely considered. We investigated different laser-fabricated spike structures using primary and immortalized cell lines of fibroblasts and osteoblasts and included quantification of the cell area, aspect ratio, and focal adhesions. Furthermore, we examined the three-dimensional cell interactions with spike topographies and developed a tailored migration assay for long-term monitoring on opaque materials. While fibroblasts and osteoblasts on small spikes retained their normal morphology, cells on medium and large spikes sank into the structures, affecting the composition of the cytoskeleton and thereby changing cell shape. Up to 14 days, migration appeared stronger on small spikes, probably as a consequence of adequate focal adhesion formation and an intact cytoskeleton, whereas human primary cells revealed differences in comparison to immortalized cell lines. The use of primary cells, analysis of the cell–implant structure interaction as well as cell migration might strengthen the evaluation of cytocompatibility and thereby improve the validity regarding the putative in vivo performance of implant material.

KW - Biomaterials

KW - Cell exclusion assay

KW - Cell proliferation

KW - Cell spreading

KW - Cytocompatibility

KW - Focal adhesion

KW - In vitro screening

KW - Primary vs. immortalized cell lines

KW - Spike structures

UR - http://www.scopus.com/inward/record.url?scp=85096023490&partnerID=8YFLogxK

U2 - 10.3390/ijms21228442

DO - 10.3390/ijms21228442

M3 - Article

C2 - 33182746

AN - SCOPUS:85096023490

VL - 21

SP - 1

EP - 19

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1661-6596

IS - 22

M1 - 8442

ER -