Cell counting based on local intensity maxima grouping for in-situ microscopy

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OriginalspracheEnglisch
Titel des Sammelwerks2014 IEEE 11th International Symposium on Biomedical Imaging, ISBI 2014
Herausgeber (Verlag)Institute of Electrical and Electronics Engineers Inc.
Seiten1344-1347
Seitenumfang4
ISBN (elektronisch)9781467319591
PublikationsstatusVeröffentlicht - 31 Juli 2014
Veranstaltung2014 IEEE 11th International Symposium on Biomedical Imaging, ISBI 2014 - Beijing, China
Dauer: 29 Apr. 20142 Mai 2014

Publikationsreihe

Name Proceedings (International Symposium on Biomedical Imaging)
ISSN (Print)1945-7928

Abstract

In this contribution, a new algorithm to estimate the cell count from an intensity image of Baby Hamster Kidney (BHK) cells captured by an in-situ microscope is proposed. Given that the local intensity maxima inside a cell share similar location and intensity values, it is proposed to find all the intensity maxima inside each cell cluster present in the image, and then group those who share similar location and intensity values. The total number of cells present in an image is estimated as the sum of the number of groups found in each cluster. The experimental results show that the average cell count improved by 79%, and that the average image processing time improved by 42%.

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Cell counting based on local intensity maxima grouping for in-situ microscopy. / Rojas, L. D.; Martinez, G.; Scheper, T.
2014 IEEE 11th International Symposium on Biomedical Imaging, ISBI 2014. Institute of Electrical and Electronics Engineers Inc., 2014. S. 1344-1347 ( Proceedings (International Symposium on Biomedical Imaging)).

Publikation: Beitrag in Buch/Bericht/Sammelwerk/KonferenzbandAufsatz in KonferenzbandForschungPeer-Review

Rojas, LD, Martinez, G & Scheper, T 2014, Cell counting based on local intensity maxima grouping for in-situ microscopy. in 2014 IEEE 11th International Symposium on Biomedical Imaging, ISBI 2014. Proceedings (International Symposium on Biomedical Imaging), Institute of Electrical and Electronics Engineers Inc., S. 1344-1347, 2014 IEEE 11th International Symposium on Biomedical Imaging, ISBI 2014, Beijing, China, 29 Apr. 2014. https://doi.org/10.1109/isbi.2014.6868126
Rojas, L. D., Martinez, G., & Scheper, T. (2014). Cell counting based on local intensity maxima grouping for in-situ microscopy. In 2014 IEEE 11th International Symposium on Biomedical Imaging, ISBI 2014 (S. 1344-1347). ( Proceedings (International Symposium on Biomedical Imaging)). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/isbi.2014.6868126
Rojas LD, Martinez G, Scheper T. Cell counting based on local intensity maxima grouping for in-situ microscopy. in 2014 IEEE 11th International Symposium on Biomedical Imaging, ISBI 2014. Institute of Electrical and Electronics Engineers Inc. 2014. S. 1344-1347. ( Proceedings (International Symposium on Biomedical Imaging)). doi: 10.1109/isbi.2014.6868126
Rojas, L. D. ; Martinez, G. ; Scheper, T. / Cell counting based on local intensity maxima grouping for in-situ microscopy. 2014 IEEE 11th International Symposium on Biomedical Imaging, ISBI 2014. Institute of Electrical and Electronics Engineers Inc., 2014. S. 1344-1347 ( Proceedings (International Symposium on Biomedical Imaging)).
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title = "Cell counting based on local intensity maxima grouping for in-situ microscopy",
abstract = "In this contribution, a new algorithm to estimate the cell count from an intensity image of Baby Hamster Kidney (BHK) cells captured by an in-situ microscope is proposed. Given that the local intensity maxima inside a cell share similar location and intensity values, it is proposed to find all the intensity maxima inside each cell cluster present in the image, and then group those who share similar location and intensity values. The total number of cells present in an image is estimated as the sum of the number of groups found in each cluster. The experimental results show that the average cell count improved by 79%, and that the average image processing time improved by 42%.",
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TY - GEN

T1 - Cell counting based on local intensity maxima grouping for in-situ microscopy

AU - Rojas, L. D.

AU - Martinez, G.

AU - Scheper, T.

PY - 2014/7/31

Y1 - 2014/7/31

N2 - In this contribution, a new algorithm to estimate the cell count from an intensity image of Baby Hamster Kidney (BHK) cells captured by an in-situ microscope is proposed. Given that the local intensity maxima inside a cell share similar location and intensity values, it is proposed to find all the intensity maxima inside each cell cluster present in the image, and then group those who share similar location and intensity values. The total number of cells present in an image is estimated as the sum of the number of groups found in each cluster. The experimental results show that the average cell count improved by 79%, and that the average image processing time improved by 42%.

AB - In this contribution, a new algorithm to estimate the cell count from an intensity image of Baby Hamster Kidney (BHK) cells captured by an in-situ microscope is proposed. Given that the local intensity maxima inside a cell share similar location and intensity values, it is proposed to find all the intensity maxima inside each cell cluster present in the image, and then group those who share similar location and intensity values. The total number of cells present in an image is estimated as the sum of the number of groups found in each cluster. The experimental results show that the average cell count improved by 79%, and that the average image processing time improved by 42%.

KW - Biomedical imaging

KW - Cell cluster segmentation

KW - Cell counting

KW - Cell image analysis

KW - Detection

KW - Grouping

KW - In-situ microscopy

KW - Local intensity maxima

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