Details
| Originalsprache | Englisch |
|---|---|
| Aufsatznummer | 11 |
| Fachzeitschrift | PLANT METHODS |
| Jahrgang | 1 |
| Ausgabenummer | 11 |
| Publikationsstatus | Veröffentlicht - 16 Nov. 2005 |
Abstract
Intact protein complexes can be separated by apparent molecular mass using a standard polyacrylamide gel electrophoresis system combining mild detergents and the dye Coomassie Blue. Referring to the blue coloured gel and the gentle method of solubilization yielding native and enzymatically active protein complexes, this technique has been named Blue-Native Polyacrylamide Gel-Electrophoresis (BN-PAGE). BN-PAGE has become the method of choice for the investigation of the respiratory protein complexes of the electron transfer chains of a range of organisms, including bacteria, yeasts, animals and plants. It allows the separation in two dimensions of extremely hydrophobic protein sets for analysis and also provides information on their native interactions. In this review we discuss the capabilities of BN-PAGE in proteomics and the wider investigation of protein:protein interactions with a focus on its use and potential in plant science.
ASJC Scopus Sachgebiete
- Biochemie, Genetik und Molekularbiologie (insg.)
- Biotechnologie
- Biochemie, Genetik und Molekularbiologie (insg.)
- Genetik
- Agrar- und Biowissenschaften (insg.)
- Pflanzenkunde
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in: PLANT METHODS, Jahrgang 1, Nr. 11, 11, 16.11.2005.
Publikation: Beitrag in Fachzeitschrift › Übersichtsarbeit › Forschung › Peer-Review
}
TY - JOUR
T1 - Blue-native PAGE in plants
T2 - A tool in analysis of protein-protein interactions
AU - Eubel, Holger
AU - Braun, Hans Peter
AU - Millar, A. Harvey
PY - 2005/11/16
Y1 - 2005/11/16
N2 - Intact protein complexes can be separated by apparent molecular mass using a standard polyacrylamide gel electrophoresis system combining mild detergents and the dye Coomassie Blue. Referring to the blue coloured gel and the gentle method of solubilization yielding native and enzymatically active protein complexes, this technique has been named Blue-Native Polyacrylamide Gel-Electrophoresis (BN-PAGE). BN-PAGE has become the method of choice for the investigation of the respiratory protein complexes of the electron transfer chains of a range of organisms, including bacteria, yeasts, animals and plants. It allows the separation in two dimensions of extremely hydrophobic protein sets for analysis and also provides information on their native interactions. In this review we discuss the capabilities of BN-PAGE in proteomics and the wider investigation of protein:protein interactions with a focus on its use and potential in plant science.
AB - Intact protein complexes can be separated by apparent molecular mass using a standard polyacrylamide gel electrophoresis system combining mild detergents and the dye Coomassie Blue. Referring to the blue coloured gel and the gentle method of solubilization yielding native and enzymatically active protein complexes, this technique has been named Blue-Native Polyacrylamide Gel-Electrophoresis (BN-PAGE). BN-PAGE has become the method of choice for the investigation of the respiratory protein complexes of the electron transfer chains of a range of organisms, including bacteria, yeasts, animals and plants. It allows the separation in two dimensions of extremely hydrophobic protein sets for analysis and also provides information on their native interactions. In this review we discuss the capabilities of BN-PAGE in proteomics and the wider investigation of protein:protein interactions with a focus on its use and potential in plant science.
KW - 2D-PAGE
KW - Coomassie
KW - Gel-based Proteomics
KW - hydrophobic proteins
KW - protein complexes
KW - solubilization
UR - http://www.scopus.com/inward/record.url?scp=33744901613&partnerID=8YFLogxK
U2 - 10.1186/1746-4811-1-11
DO - 10.1186/1746-4811-1-11
M3 - Review article
AN - SCOPUS:33744901613
VL - 1
JO - PLANT METHODS
JF - PLANT METHODS
SN - 1746-4811
IS - 11
M1 - 11
ER -