Blue native PAGE

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OriginalspracheEnglisch
Seiten (von - bis)418-428
Seitenumfang11
FachzeitschriftNature protocols
Jahrgang1
Ausgabenummer1
PublikationsstatusVeröffentlicht - 27 Juni 2006

Abstract

Blue native PAGE (BN-PAGE) can be used for one-step isolation of protein complexes from biological membranes and total cell and tissue homogenates. It can also be used to determine native protein masses and oligomeric states and to identify physiological protein-protein interactions. Native complexes are recovered from gels by electroelution or diffusion and are used for 2D crystallization and electron microscopy or analyzed by in-gel activity assays or by native electroblotting and immunodetection. In this protocol, we describe methodology to perform BN-PAGE followed by (i) native extraction or native electroblotting of separated proteins, or (ii) a second dimension of tricine-SDS-PAGE or modified BN-PAGE, or (iii) a second dimension of isoelectric focusing (IEF) followed by a third dimension of tricine-SDS-PAGE for the separation of subunits of complexes. These protocols for 2D and 3D PAGE can be completed in 2 and 3 days.

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Blue native PAGE. / Wittig, Ilka; Braun, Hans Peter; Schägger, Hermann.
in: Nature protocols, Jahrgang 1, Nr. 1, 27.06.2006, S. 418-428.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Wittig, I, Braun, HP & Schägger, H 2006, 'Blue native PAGE', Nature protocols, Jg. 1, Nr. 1, S. 418-428. https://doi.org/10.1038/nprot.2006.62
Wittig I, Braun HP, Schägger H. Blue native PAGE. Nature protocols. 2006 Jun 27;1(1):418-428. doi: 10.1038/nprot.2006.62
Wittig, Ilka ; Braun, Hans Peter ; Schägger, Hermann. / Blue native PAGE. in: Nature protocols. 2006 ; Jahrgang 1, Nr. 1. S. 418-428.
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