Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 539-544 |
| Seitenumfang | 6 |
| Fachzeitschrift | Journal of Proteomics |
| Jahrgang | 72 |
| Ausgabenummer | 3 |
| Publikationsstatus | Veröffentlicht - 8 Jan. 2009 |
Abstract
Differential gel electrophoresis (DIGE) is based on pre-labeling of different protein fractions and their subsequent co-electrophoresis in a single gel. Cyanine based "CyDye DIGE Fluor minimal dyes" are used for the labeling reaction and 2D IEF/SDS PAGE is the preferential electrophoresis system for protein separation. The DIGE technology allows elimination of inconsistencies based on gel to gel variations and furthermore allows exact quantification of proteins separated by gel electrophoresis. Here we report applications of the DIGE technology in combination with another 2D gel system, Blue native/SDS PAGE. "Blue native DIGE" offers (i) systematic and quantitative comparison of protein complexes of related protein fractions, (ii) structural investigation of protein complexes, (iii) assignment of protein complexes to subcellular fractions like organelles and (iv) electrophoretic mapping of isoforms of subunits of protein complexes with respect to a larger proteome. The potential of "Blue native DIGE" is illustrated by analysis of organellar fractions from the plant Arabidopsis thaliana and the alga Polytomella. Use of the DIGE technology for topological investigations is discussed.
Zitieren
- Standard
- Harvard
- Apa
- Vancouver
- BibTex
- RIS
in: Journal of Proteomics, Jahrgang 72, Nr. 3, 08.01.2009, S. 539-544.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Blue native DIGE as a tool for comparative analyses of protein complexes
AU - Heinemeyer, Jesco
AU - Scheibe, Burghardt
AU - Schmitz, Udo
AU - Braun, Hans Peter
PY - 2009/1/8
Y1 - 2009/1/8
N2 - Differential gel electrophoresis (DIGE) is based on pre-labeling of different protein fractions and their subsequent co-electrophoresis in a single gel. Cyanine based "CyDye DIGE Fluor minimal dyes" are used for the labeling reaction and 2D IEF/SDS PAGE is the preferential electrophoresis system for protein separation. The DIGE technology allows elimination of inconsistencies based on gel to gel variations and furthermore allows exact quantification of proteins separated by gel electrophoresis. Here we report applications of the DIGE technology in combination with another 2D gel system, Blue native/SDS PAGE. "Blue native DIGE" offers (i) systematic and quantitative comparison of protein complexes of related protein fractions, (ii) structural investigation of protein complexes, (iii) assignment of protein complexes to subcellular fractions like organelles and (iv) electrophoretic mapping of isoforms of subunits of protein complexes with respect to a larger proteome. The potential of "Blue native DIGE" is illustrated by analysis of organellar fractions from the plant Arabidopsis thaliana and the alga Polytomella. Use of the DIGE technology for topological investigations is discussed.
AB - Differential gel electrophoresis (DIGE) is based on pre-labeling of different protein fractions and their subsequent co-electrophoresis in a single gel. Cyanine based "CyDye DIGE Fluor minimal dyes" are used for the labeling reaction and 2D IEF/SDS PAGE is the preferential electrophoresis system for protein separation. The DIGE technology allows elimination of inconsistencies based on gel to gel variations and furthermore allows exact quantification of proteins separated by gel electrophoresis. Here we report applications of the DIGE technology in combination with another 2D gel system, Blue native/SDS PAGE. "Blue native DIGE" offers (i) systematic and quantitative comparison of protein complexes of related protein fractions, (ii) structural investigation of protein complexes, (iii) assignment of protein complexes to subcellular fractions like organelles and (iv) electrophoretic mapping of isoforms of subunits of protein complexes with respect to a larger proteome. The potential of "Blue native DIGE" is illustrated by analysis of organellar fractions from the plant Arabidopsis thaliana and the alga Polytomella. Use of the DIGE technology for topological investigations is discussed.
KW - Arabidopsis thaliana
KW - Blue native PAGE
KW - Differential gel electrophoresis
KW - DIGE
KW - Mitochondria
KW - Protein complexes
UR - http://www.scopus.com/inward/record.url?scp=63349099933&partnerID=8YFLogxK
U2 - 10.15488/11663
DO - 10.15488/11663
M3 - Article
C2 - 19166986
AN - SCOPUS:63349099933
VL - 72
SP - 539
EP - 544
JO - Journal of Proteomics
JF - Journal of Proteomics
SN - 1874-3919
IS - 3
ER -