TY - JOUR

T1 - Biotransformation of citronellol by the basidiomycete Cystoderma carcharias in an aerated-membrane bioreactor

AU - Onken, J.

AU - Berger, R. G.

N1 - Funding information: Acknowledgement This work was supported by the EU project BIO4-CT95-0049 and by the Fonds der Chemischen Industrie, Frankfurt.

PY - 1999/2

Y1 - 1999/2

N2 - The basidiomycete Cystoderma carcharias transformed citronellol into 3,7-dimethyl-1,6,7-octane-triol as the main product. 3,7-Dimethyl-6,7-epoxy- 1-octanol was identified as important intermediary product of the biotransformation, and the allylic diols 2,6-dimethyl-2-octene-1,8-diol, 3,7- dimethyl-5-octene-1,7-diol and 3,7-dimethyl-7-octene-1,6-diol were found to be minor products. Microbial formation of rose oxide, a flavour-impact component, was observed for the first time. The formation of the main products was inhibited by 70% after addition of 0.1 mmol l-1 cytochrome monooxygenase inhibitors. Formation of 3,7-dimethyl-1,6,7-octanetriol was effective in a bioreactor with aeration over a coil of a hydrophobic microporous polypropene capillary membrane. Production rates of up to 150 mg l-1 day-1 were reached and led tO a product concentration of 866 mg l- 1 (conversion rate: 52%). The total loss of the added volatile substrate via the exhaust air was 4.5% when this aeration method was used.

AB - The basidiomycete Cystoderma carcharias transformed citronellol into 3,7-dimethyl-1,6,7-octane-triol as the main product. 3,7-Dimethyl-6,7-epoxy- 1-octanol was identified as important intermediary product of the biotransformation, and the allylic diols 2,6-dimethyl-2-octene-1,8-diol, 3,7- dimethyl-5-octene-1,7-diol and 3,7-dimethyl-7-octene-1,6-diol were found to be minor products. Microbial formation of rose oxide, a flavour-impact component, was observed for the first time. The formation of the main products was inhibited by 70% after addition of 0.1 mmol l-1 cytochrome monooxygenase inhibitors. Formation of 3,7-dimethyl-1,6,7-octanetriol was effective in a bioreactor with aeration over a coil of a hydrophobic microporous polypropene capillary membrane. Production rates of up to 150 mg l-1 day-1 were reached and led tO a product concentration of 866 mg l- 1 (conversion rate: 52%). The total loss of the added volatile substrate via the exhaust air was 4.5% when this aeration method was used.

UR - http://www.scopus.com/inward/record.url?scp=0033025939&partnerID=8YFLogxK

U2 - 10.1007/s002530051376

DO - 10.1007/s002530051376

M3 - Article

C2 - 10091320

AN - SCOPUS:0033025939

VL - 51

SP - 158

EP - 163

JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

SN - 0175-7598

IS - 2

ER -