Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 646-658 |
| Seitenumfang | 13 |
| Fachzeitschrift | Journal of Biophotonics |
| Jahrgang | 8 |
| Ausgabenummer | 8 |
| Publikationsstatus | Veröffentlicht - 9 Okt. 2014 |
Abstract
Gold nanoparticle mediated (GNOME) laser transfection is a powerful technique to deliver small biologically relevant molecules into cells. However, the transfection of larger and especially negatively charged DNA remains challenging. The efficiency for pDNA was 0.57% using parameter that does not influence the endo- and exogenous DNA. In order to gain a deeper understanding of the actual molecule uptake process, the uptake efficiency was determined using molecules of different sizes. It was evaluated that uncharged dextran molecules (2000 kDa) were delivered with an efficiency of 68%. The intracellular distribution of injected molecules was visualized and larger molecules were primary found in the cytoplasm. Patch clamp measurements suggested a permeabilization time up to 15 minutes. The uptake efficiency depended on the size and charge of the molecule to deliver as well as the cell size. A minor role for transfection plays the cell type since primary stem cells were successfully transfected.
ASJC Scopus Sachgebiete
- Chemie (insg.)
- Allgemeine Chemie
- Werkstoffwissenschaften (insg.)
- Allgemeine Materialwissenschaften
- Biochemie, Genetik und Molekularbiologie (insg.)
- Allgemeine Biochemie, Genetik und Molekularbiologie
- Ingenieurwesen (insg.)
- Allgemeiner Maschinenbau
- Physik und Astronomie (insg.)
- Allgemeine Physik und Astronomie
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in: Journal of Biophotonics, Jahrgang 8, Nr. 8, 09.10.2014, S. 646-658.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Biophysical effects in off-resonant gold nanoparticle mediated (GNOME) laser transfection of cell lines, primary- and stem cells using fs laser pulses
AU - Schomaker, Markus
AU - Killian, Doreen
AU - Willenbrock, Saskia
AU - Heinemann, Dag
AU - Kalies, Stefan
AU - Ngezahayo, Anaclet
AU - Nolte, Ingo
AU - Ripken, Tammo
AU - Junghanß, Christian
AU - Meyer, Heiko
AU - Murua Escobar, Hugo
AU - Heisterkamp, Alexander
PY - 2014/10/9
Y1 - 2014/10/9
N2 - Gold nanoparticle mediated (GNOME) laser transfection is a powerful technique to deliver small biologically relevant molecules into cells. However, the transfection of larger and especially negatively charged DNA remains challenging. The efficiency for pDNA was 0.57% using parameter that does not influence the endo- and exogenous DNA. In order to gain a deeper understanding of the actual molecule uptake process, the uptake efficiency was determined using molecules of different sizes. It was evaluated that uncharged dextran molecules (2000 kDa) were delivered with an efficiency of 68%. The intracellular distribution of injected molecules was visualized and larger molecules were primary found in the cytoplasm. Patch clamp measurements suggested a permeabilization time up to 15 minutes. The uptake efficiency depended on the size and charge of the molecule to deliver as well as the cell size. A minor role for transfection plays the cell type since primary stem cells were successfully transfected.
AB - Gold nanoparticle mediated (GNOME) laser transfection is a powerful technique to deliver small biologically relevant molecules into cells. However, the transfection of larger and especially negatively charged DNA remains challenging. The efficiency for pDNA was 0.57% using parameter that does not influence the endo- and exogenous DNA. In order to gain a deeper understanding of the actual molecule uptake process, the uptake efficiency was determined using molecules of different sizes. It was evaluated that uncharged dextran molecules (2000 kDa) were delivered with an efficiency of 68%. The intracellular distribution of injected molecules was visualized and larger molecules were primary found in the cytoplasm. Patch clamp measurements suggested a permeabilization time up to 15 minutes. The uptake efficiency depended on the size and charge of the molecule to deliver as well as the cell size. A minor role for transfection plays the cell type since primary stem cells were successfully transfected.
KW - DNA
KW - GNOME laser transfection
KW - Gold nanoparticles
KW - Molecule size
KW - Stem cells
UR - http://www.scopus.com/inward/record.url?scp=84938559527&partnerID=8YFLogxK
U2 - 10.1002/jbio.201400065
DO - 10.1002/jbio.201400065
M3 - Article
C2 - 25302483
AN - SCOPUS:84938559527
VL - 8
SP - 646
EP - 658
JO - Journal of Biophotonics
JF - Journal of Biophotonics
SN - 1864-063X
IS - 8
ER -