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| Titel in Übersetzung | Whole-Cell Biocatalytical Production of the Sesquiterpene Presilphiperfolan-8b-ol in Pathway-Engineered Escherichia coli |
|---|---|
| Originalsprache | Deutsch |
| Seiten (von - bis) | 576-586 |
| Seitenumfang | 11 |
| Fachzeitschrift | Chemie-Ingenieur-Technik |
| Jahrgang | 95 |
| Ausgabenummer | 4 |
| Publikationsstatus | Veröffentlicht - 22 März 2023 |
Abstract
Fermentative whole-cell production in Escherichia coli offers the option of producing complex natural products such as terpenes from simple, inexpensive, and sustainable carbon sources. The aim of this study was to develop such a process for the synthesis of presilphiperfolan-8β-ol (PSP). The biosynthetic pathway of this tricyclic sesquiterpene alcohol was successfully introduced into E. coli. The resulting multi-plasmid strain was able to produce farnesyl pyrophosphate in vivo via the mevalonate pathway, which was then converted to PSP by the sesquiterpene cyclase BcBOT2. The product, which is mainly secreted into the culture medium, was identified via GC-MS and quantified via GC-FID. The constructed strain produced 10 mg L−1 PSP in 48 h at 20 °C directly from carbon feedstock.
Schlagwörter
- BcBOT2, Fermentation, Presilphiperfolan-8β-ol, Sesquiterpenes, Whole-cell production
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in: Chemie-Ingenieur-Technik, Jahrgang 95, Nr. 4, 22.03.2023, S. 576-586.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Biokatalytische Ganzzellproduktion des Sesquiterpens Presilphiperfolan-8β-ol in stoffwechseloptimierten Escherichia coli
AU - Wildhagen, Maik
AU - Pudenz, Tabea
AU - Nguyen, Trang
AU - Kirschning, Andreas
AU - Beutel, Sascha
N1 - Funding information: Für die technische Unterstützung wird Martina Weiß und Martin Pähler gedankt sowie Ulrich Krings von Institut für Lebensmittelchemie für die Unterstützung in der GC-MS Analytik. Dem nds. MWK wird gedankt für die Förderung der wissenschaftlichen Arbeiten im Rahmen des ABA-Projektes (FKZ: ZN3822) aus Mitteln des nds. VW-Vorab. Open Access Veröffentlichung ermöglicht und organisiert durch Projekt DEAL.
PY - 2023/3/22
Y1 - 2023/3/22
N2 - Fermentative whole-cell production in Escherichia coli offers the option of producing complex natural products such as terpenes from simple, inexpensive, and sustainable carbon sources. The aim of this study was to develop such a process for the synthesis of presilphiperfolan-8β-ol (PSP). The biosynthetic pathway of this tricyclic sesquiterpene alcohol was successfully introduced into E. coli. The resulting multi-plasmid strain was able to produce farnesyl pyrophosphate in vivo via the mevalonate pathway, which was then converted to PSP by the sesquiterpene cyclase BcBOT2. The product, which is mainly secreted into the culture medium, was identified via GC-MS and quantified via GC-FID. The constructed strain produced 10 mg L−1 PSP in 48 h at 20 °C directly from carbon feedstock.
AB - Fermentative whole-cell production in Escherichia coli offers the option of producing complex natural products such as terpenes from simple, inexpensive, and sustainable carbon sources. The aim of this study was to develop such a process for the synthesis of presilphiperfolan-8β-ol (PSP). The biosynthetic pathway of this tricyclic sesquiterpene alcohol was successfully introduced into E. coli. The resulting multi-plasmid strain was able to produce farnesyl pyrophosphate in vivo via the mevalonate pathway, which was then converted to PSP by the sesquiterpene cyclase BcBOT2. The product, which is mainly secreted into the culture medium, was identified via GC-MS and quantified via GC-FID. The constructed strain produced 10 mg L−1 PSP in 48 h at 20 °C directly from carbon feedstock.
KW - BcBOT2
KW - Fermentation
KW - Presilphiperfolan-8β-ol
KW - Sesquiterpenes
KW - Whole-cell production
UR - http://www.scopus.com/inward/record.url?scp=85147814662&partnerID=8YFLogxK
U2 - 10.1002/cite.202200115
DO - 10.1002/cite.202200115
M3 - Artikel
AN - SCOPUS:85147814662
VL - 95
SP - 576
EP - 586
JO - Chemie-Ingenieur-Technik
JF - Chemie-Ingenieur-Technik
SN - 0009-286X
IS - 4
ER -