TY - JOUR

T1 - Bioinstructive Coatings for Hematopoietic Stem Cell Expansion Based on Chemical Vapor Deposition Copolymerization

AU - Winkler, Anna-Lena

AU - Koenig, Meike

AU - Welle, Alexander

AU - Trouillet, Vanessa

AU - Kratzer, Domenic

AU - Hussal, Christoph

AU - Lahann, Joerg

AU - Lee-Thedieck, Cornelia

N1 - Funding information: The project was funded by the BMBF NanoMatFutur Program (FKZ 13N12968). A.W., M.K., C.H., J.L., and C.L.T. acknowledge support by the program “Biointerfaces in Technology and Medicine” of the Helmholtz Association. C.H. and J.L. thank the German Research Foundation (DFG) for financial support within the frame of the collaborative research center SFB 1176 (Project B3). The authors thank Saskia Kraus for excellent technical assistance. The K-Alpha+ instrument was financially supported by the Federal Ministry of Economics and Technology.

PY - 2017/10/9

Y1 - 2017/10/9

N2 - We report the chemical vapor deposition (CVD) of dual-functional polymer films for the specific and orthogonal immobilization of two biomolecules (notch ligand delta-like 1 (DLL1) and an RGD-peptide) that govern the fate of hematopoietic stem and progenitor cells. The composition of the CVD polymer and thus the biomolecule ratio can be tailored to investigate and optimize the influence of the relative surface concentrations of biomolecules on stem cell behavior. Prior to cell experiments, all surfaces were characterized by infrared reflection adsorption spectroscopy, time-of-flight secondary ion mass spectrometry, and X-ray photoelectron spectroscopy to confirm the presence of both biomolecules. In a proof-of-principle stem cell culture study, we show that all polymer surfaces are cytocompatible and that the proliferation of the hematopoietic stem and progenitor cells is predominantly influenced by the surface concentration of immobilized DLL1.

AB - We report the chemical vapor deposition (CVD) of dual-functional polymer films for the specific and orthogonal immobilization of two biomolecules (notch ligand delta-like 1 (DLL1) and an RGD-peptide) that govern the fate of hematopoietic stem and progenitor cells. The composition of the CVD polymer and thus the biomolecule ratio can be tailored to investigate and optimize the influence of the relative surface concentrations of biomolecules on stem cell behavior. Prior to cell experiments, all surfaces were characterized by infrared reflection adsorption spectroscopy, time-of-flight secondary ion mass spectrometry, and X-ray photoelectron spectroscopy to confirm the presence of both biomolecules. In a proof-of-principle stem cell culture study, we show that all polymer surfaces are cytocompatible and that the proliferation of the hematopoietic stem and progenitor cells is predominantly influenced by the surface concentration of immobilized DLL1.

UR - http://www.scopus.com/inward/record.url?scp=85031292503&partnerID=8YFLogxK

U2 - 10.1021/acs.biomac.7b00743

DO - 10.1021/acs.biomac.7b00743

M3 - Article

VL - 18

SP - 3089

EP - 3098

JO - BIOMACROMOLECULES

JF - BIOMACROMOLECULES

SN - 1525-7797

IS - 10

ER -