TY - CHAP

T1 - Biofilm formation behaviors formed by E. Coli under weak alternating electromagnetic fields

AU - Kanematsu, Hideyuki

AU - Katsuragawa, Takaya

AU - Barry, Dana

AU - Yokoi, Keiya

AU - Umeki, Senshin

AU - Miura, Hidekazu

AU - Suzuki, Koji

AU - Ogawa, Akiko

AU - Hirai, Nobumitsu

AU - Kougo, Takeshi

AU - Kuroda, Daisuke

AU - Zimmermann, Stefan

N1 - Funding Information: The research project was carried out by the financial support of New Energy and Industrial Technology Development Organization (NEDO). The project was entitled “Research and development of geothermal power generation technique/Innovative technology development for expanding introduction of geothermal power generation/"Research and development of physical removing scale technology for geothermal power generation with hot spring heat utilization”. We greatly appreciate the organization for their constant financial support. And this work was also supported by JSPS KAKENHI (Grants-in-Aid for Scientific Research from Japan Society for the Promotion of Science) Grant Number 17K06826. The current investigation was supported by the headquarters of the National Institute of Technology (Kosen) financially (Network Formation Project, 2017). We appreciate them very highly. And we very highly appreciate Mitsubishi Electric Corporation and its research center of advanced technology and the Society of Industrial Technology for Antimicrobial Articles (SIAA) for their useful advice and financial support. Also we would like to thank the Japan Food Research Laboratory (JFRL) for their constant encouragement and useful advice.

PY - 2019/10/30

Y1 - 2019/10/30

N2 - This chapter describes how formation behaviors of biofilm formed by under weak alternating electromagnetic fields. Biofilms form on materials as a result of bacterial activity. Depending on the situation, these biofilms can have a variety of negative effects on these materials. Therefore, biofilms need to be controlled by the use of appropriate industrial methods. The purpose of this study is to suppress the formation of biofilms by applying weak alternating electromagnetic fields. The project and its results are presented and discussed. For this work, E.coli (a representative gramnegative bacteria present in our daily lives) was selected as the bacteria for forming biofilm. In each case, six samples were prepared. Electromagnetic waves were applied to three of them, while the other three samples did not receive electromagnetic waves. All samples were immersed in a liquid culture (LB culture) at 25 degrees for 5 days. Frequencies used in these experiments were 4 kHz, 8 kHz, 12 kHz and 16 kHz. The biofilm formation behavior was analyzed by staining with crystal violet and Raman spectroscopy. It seemed to depend on the bacteria and the frequencies used. The results are discussed from the viewpoint of the effect of electromagnetic fields.

AB - This chapter describes how formation behaviors of biofilm formed by under weak alternating electromagnetic fields. Biofilms form on materials as a result of bacterial activity. Depending on the situation, these biofilms can have a variety of negative effects on these materials. Therefore, biofilms need to be controlled by the use of appropriate industrial methods. The purpose of this study is to suppress the formation of biofilms by applying weak alternating electromagnetic fields. The project and its results are presented and discussed. For this work, E.coli (a representative gramnegative bacteria present in our daily lives) was selected as the bacteria for forming biofilm. In each case, six samples were prepared. Electromagnetic waves were applied to three of them, while the other three samples did not receive electromagnetic waves. All samples were immersed in a liquid culture (LB culture) at 25 degrees for 5 days. Frequencies used in these experiments were 4 kHz, 8 kHz, 12 kHz and 16 kHz. The biofilm formation behavior was analyzed by staining with crystal violet and Raman spectroscopy. It seemed to depend on the bacteria and the frequencies used. The results are discussed from the viewpoint of the effect of electromagnetic fields.

UR - http://www.scopus.com/inward/record.url?scp=85075378973&partnerID=8YFLogxK

U2 - 10.1002/9781119631460.ch18

DO - 10.1002/9781119631460.ch18

M3 - Contribution to book/anthology

SN - 9781119631460

T3 - Ceramic Transactions

SP - 195

EP - 210

BT - Advances in Ceramics for Environmental, Functional, Structural and Energy Applications II

A2 - Bhalla, Amar

A2 - Mahmoud, Morsi

A2 - Bansal, Narottam

A2 - Suvorov, Danilo

A2 - Guo, Ruyan

A2 - Ubic, Rick

A2 - Amoroso, Jake

A2 - Trivelpiece, Coy

A2 - Manjooran, Navin Jose

A2 - Pickrell, Gary

A2 - Agrawal, Dinesh

PB - John Wiley & Sons Inc.

ER -