Bioconversion of (+)-valencene in submerged cultures of the ascomycete Chaetomium globosum

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Autoren

  • Rüdiger Kaspera
  • Ulrich Krings
  • Tsevegsuren Nanzad
  • Ralf G. Berger

Organisationseinheiten

Externe Organisationen

  • National University of Mongolia
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Details

OriginalspracheEnglisch
Seiten (von - bis)477-483
Seitenumfang7
FachzeitschriftApplied Microbiology and Biotechnology
Jahrgang67
Ausgabenummer4
PublikationsstatusVeröffentlicht - 16 Dez. 2004

Abstract

Submerged cultures of the ascomycete Chaetomium globosum oxidised the exogenous sesquiterpene (+)-valencene to nootkatone via the stereoselective generation of α-nootkatol. Inhibition experiments suggested that the first introduction of oxygen, the rate-limiting step of the bioconversion, may have been catalysed by a cytochrome-P450-monooxygenase. However, nootkatone was not the final metabolite: further flavour-active and inactive, non-volatile oxidation products were identified. (+)-Valencene and the flavour-active mono-oxyfunctionalised transformation products, α-nootkatol, nootkatone, and valencene-11,12-epoxide accumulated preferably inside the fungal cells. Di- and poly-oxygenated products, such as nootkatone-11,12-epoxide, were found solely in the culture medium, indicating an active transport of these metabolites into the extracellular compartment during (+)-valencene detoxification. These metabolic properties may have contributed to the high tolerance of the fungus towards the exogenous hydrocarbon.

ASJC Scopus Sachgebiete

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Bioconversion of (+)-valencene in submerged cultures of the ascomycete Chaetomium globosum. / Kaspera, Rüdiger; Krings, Ulrich; Nanzad, Tsevegsuren et al.
in: Applied Microbiology and Biotechnology, Jahrgang 67, Nr. 4, 16.12.2004, S. 477-483.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Kaspera R, Krings U, Nanzad T, Berger RG. Bioconversion of (+)-valencene in submerged cultures of the ascomycete Chaetomium globosum. Applied Microbiology and Biotechnology. 2004 Dez 16;67(4):477-483. doi: 10.1007/s00253-004-1794-0
Kaspera, Rüdiger ; Krings, Ulrich ; Nanzad, Tsevegsuren et al. / Bioconversion of (+)-valencene in submerged cultures of the ascomycete Chaetomium globosum. in: Applied Microbiology and Biotechnology. 2004 ; Jahrgang 67, Nr. 4. S. 477-483.
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AU - Nanzad, Tsevegsuren

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