TY - JOUR

T1 - Bioavailability of rosehip (Rosa canina L.) infusion phenolics prepared by thermal, pulsed electric field and high pressure processing

AU - Ozkan, Gulay

AU - Esatbeyoglu, Tuba

AU - Capanoglu, Esra

N1 - Funding Information: The publication of this article was funded by the Open Access Fund of Leibniz Universität Hannover. The authors would like to acknowledge Gerald Dräger for his guidance during LC-MS analysis and Kemal Aganovic from the German Institute of Food Technologies DIL for providing the HPP and PEF devices.

PY - 2022/7/1

Y1 - 2022/7/1

N2 - In this study, the in vitro bioavailability of rosehip infusion phenolics, mainly catechin, as a response to conventional and non-thermal treatments by combining gastrointestinal digestion and a Caco-2 cell culture model, was investigated. After application of thermal treatment (TT, 85 ◦C/10 min), high pressure (HPP, 600 MPa/5 min) or pulsed electric field (PEF, 15 kJ/kg) processing, all samples were subjected to simulated gastrointestinal digestion. Then, the amount of maximum non-toxic digest ratio was determined by the cytotoxicity sulforhodamine B (SRB) assay. Next, Caco-2 cells were exposed to 1:5 (v/v) times diluted digests in order to simulate the transepithelial transportation of catechin. Results showed that non-thermally processed samples (5.19 and 4.62% for HPP and PEF, respectively) exhibited greater transportation across the epithelial cell layer compared to than that of the TT-treated sample (3.42%). The present study highlighted that HPP and PEF, as non-thermal treatments at optimized conditions for infusions or beverages, can be utilized in order to enhance the nutritional quality of the final products.

AB - In this study, the in vitro bioavailability of rosehip infusion phenolics, mainly catechin, as a response to conventional and non-thermal treatments by combining gastrointestinal digestion and a Caco-2 cell culture model, was investigated. After application of thermal treatment (TT, 85 ◦C/10 min), high pressure (HPP, 600 MPa/5 min) or pulsed electric field (PEF, 15 kJ/kg) processing, all samples were subjected to simulated gastrointestinal digestion. Then, the amount of maximum non-toxic digest ratio was determined by the cytotoxicity sulforhodamine B (SRB) assay. Next, Caco-2 cells were exposed to 1:5 (v/v) times diluted digests in order to simulate the transepithelial transportation of catechin. Results showed that non-thermally processed samples (5.19 and 4.62% for HPP and PEF, respectively) exhibited greater transportation across the epithelial cell layer compared to than that of the TT-treated sample (3.42%). The present study highlighted that HPP and PEF, as non-thermal treatments at optimized conditions for infusions or beverages, can be utilized in order to enhance the nutritional quality of the final products.

KW - Caco-2 cell culture

KW - LC-MS

KW - bioactive compounds

KW - metabolic fate

KW - non-thermal processing

KW - transepithelial transport

UR - http://www.scopus.com/inward/record.url?scp=85133501131&partnerID=8YFLogxK

U2 - 10.3390/foods11131955

DO - 10.3390/foods11131955

M3 - Article

VL - 11

JO - Foods

JF - Foods

IS - 13

M1 - 1955

ER -