Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 726-737 |
| Seitenumfang | 12 |
| Fachzeitschrift | Trends in Biochemical Sciences |
| Jahrgang | 42 |
| Ausgabenummer | 9 |
| Frühes Online-Datum | 27 Feb. 2017 |
| Publikationsstatus | Veröffentlicht - Sept. 2017 |
Abstract
Bacterial inclusion bodies (IBs) are functional, non-toxic amyloids occurring in recombinant bacteria showing analogies with secretory granules of the mammalian endocrine system. The scientific interest in these mesoscale protein aggregates has been historically masked by their status as a hurdle in recombinant protein production. However, progressive understanding of how the cell handles the quality of recombinant polypeptides and the main features of their intriguing molecular organization has stimulated the interest in inclusion bodies and spurred their use in diverse technological fields. The engineering and tailoring of IBs as functional protein particles for materials science and biomedicine is a good example of how formerly undesired bacterial byproducts can be rediscovered as promising functional materials for a broad spectrum of applications. Bacterial IBs are mesoscale protein aggregates commonly observed in recombinant bacteria, primarily formed by recombinant protein. IBs create a bottleneck for the production of soluble protein species, and many genetic and process-based strategies have been developed to minimize their formation. The recombinant protein in IBs is partially found in an amyloidal form, which entraps non-amyloidal functional protein and offers mechanical stability. IBs naturally penetrate mammalian cells, and can be targeted to specific receptors. Upon internalization, functional protein is released, mimicking the function of secretory granules from the mammalian endocrine system. The applications of IBs as functional materials are increasing and include roles as reusable catalysts, drug delivery systems, and functional topographies in tissue engineering. The production of IBs in endotoxin-free microorganisms may extend these and additional uses.
ASJC Scopus Sachgebiete
- Biochemie, Genetik und Molekularbiologie (insg.)
- Biochemie
- Biochemie, Genetik und Molekularbiologie (insg.)
- Molekularbiologie
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in: Trends in Biochemical Sciences, Jahrgang 42, Nr. 9, 09.2017, S. 726-737.
Publikation: Beitrag in Fachzeitschrift › Übersichtsarbeit › Forschung › Peer-Review
}
TY - JOUR
T1 - Bacterial Inclusion Bodies
T2 - Discovering Their Better Half
AU - Rinas, Ursula
AU - Garcia-Fruitós, Elena
AU - Corchero, José Luis
AU - Vázquez, Esther
AU - Seras-Franzoso, Joaquin
AU - Villaverde, Antonio
N1 - Publisher Copyright: © 2017 Elsevier Ltd Copyright: Copyright 2018 Elsevier B.V., All rights reserved.
PY - 2017/9
Y1 - 2017/9
N2 - Bacterial inclusion bodies (IBs) are functional, non-toxic amyloids occurring in recombinant bacteria showing analogies with secretory granules of the mammalian endocrine system. The scientific interest in these mesoscale protein aggregates has been historically masked by their status as a hurdle in recombinant protein production. However, progressive understanding of how the cell handles the quality of recombinant polypeptides and the main features of their intriguing molecular organization has stimulated the interest in inclusion bodies and spurred their use in diverse technological fields. The engineering and tailoring of IBs as functional protein particles for materials science and biomedicine is a good example of how formerly undesired bacterial byproducts can be rediscovered as promising functional materials for a broad spectrum of applications. Bacterial IBs are mesoscale protein aggregates commonly observed in recombinant bacteria, primarily formed by recombinant protein. IBs create a bottleneck for the production of soluble protein species, and many genetic and process-based strategies have been developed to minimize their formation. The recombinant protein in IBs is partially found in an amyloidal form, which entraps non-amyloidal functional protein and offers mechanical stability. IBs naturally penetrate mammalian cells, and can be targeted to specific receptors. Upon internalization, functional protein is released, mimicking the function of secretory granules from the mammalian endocrine system. The applications of IBs as functional materials are increasing and include roles as reusable catalysts, drug delivery systems, and functional topographies in tissue engineering. The production of IBs in endotoxin-free microorganisms may extend these and additional uses.
AB - Bacterial inclusion bodies (IBs) are functional, non-toxic amyloids occurring in recombinant bacteria showing analogies with secretory granules of the mammalian endocrine system. The scientific interest in these mesoscale protein aggregates has been historically masked by their status as a hurdle in recombinant protein production. However, progressive understanding of how the cell handles the quality of recombinant polypeptides and the main features of their intriguing molecular organization has stimulated the interest in inclusion bodies and spurred their use in diverse technological fields. The engineering and tailoring of IBs as functional protein particles for materials science and biomedicine is a good example of how formerly undesired bacterial byproducts can be rediscovered as promising functional materials for a broad spectrum of applications. Bacterial IBs are mesoscale protein aggregates commonly observed in recombinant bacteria, primarily formed by recombinant protein. IBs create a bottleneck for the production of soluble protein species, and many genetic and process-based strategies have been developed to minimize their formation. The recombinant protein in IBs is partially found in an amyloidal form, which entraps non-amyloidal functional protein and offers mechanical stability. IBs naturally penetrate mammalian cells, and can be targeted to specific receptors. Upon internalization, functional protein is released, mimicking the function of secretory granules from the mammalian endocrine system. The applications of IBs as functional materials are increasing and include roles as reusable catalysts, drug delivery systems, and functional topographies in tissue engineering. The production of IBs in endotoxin-free microorganisms may extend these and additional uses.
UR - http://www.scopus.com/inward/record.url?scp=85013845732&partnerID=8YFLogxK
U2 - 10.1016/j.tibs.2017.01.005
DO - 10.1016/j.tibs.2017.01.005
M3 - Review article
C2 - 28254353
AN - SCOPUS:85013845732
VL - 42
SP - 726
EP - 737
JO - Trends in Biochemical Sciences
JF - Trends in Biochemical Sciences
SN - 0968-0004
IS - 9
ER -