Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 1351-1356 |
| Seitenumfang | 6 |
| Fachzeitschrift | Proceedings of the National Academy of Sciences of the United States of America |
| Jahrgang | 102 |
| Ausgabenummer | 5 |
| Frühes Online-Datum | 21 Jan. 2005 |
| Publikationsstatus | Veröffentlicht - 1 Feb. 2005 |
| Extern publiziert | Ja |
Abstract
The CD1 family of proteins binds self and foreign glycolipids for presentation to CD1-restricted T cells. To identify previously uncharacterized active CD1 ligands, especially those of microbial origin, numerous glycolipids were synthesized and tested for their ability to stimulate mouse and human natural killer T (NKT) cells. They included analogs of the well known NKT cell agonist α-galactosyl ceramide (α-GalCer), bacterial glycolipids, and variations of the self-glycolipid, sulfatide. Bacterial glycolipids, α-galacturonosyl-ceramides from Sphingomonas wittichii, although structurally similar to α-GalCer, have significant differences in the sugar head group as well as the ceramide portion. The Sphingomonas glycosphingolipids (GSLs) and sulfatide variants were shown to activate human NKT cells as measured by IL-4 and IFN-γ secretion. Moreover, CD1d-dimer staining revealed human NKT cell reactivity toward these GSLs and to the sulfatides in a fashion comparable with α-GalCer. Because α-GalCer is a marine-sponge-derived ligand, our study here shows that bacterium-derived antigens are also able to stimulate mouse and human NKT cells.
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in: Proceedings of the National Academy of Sciences of the United States of America, Jahrgang 102, Nr. 5, 01.02.2005, S. 1351-1356.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Bacterial glycolipids and analogs as antigens for CD1d-restricted NKT cells
AU - Wu, Douglass
AU - Xing, Guo Wen
AU - Poles, Michael A.
AU - Horowitz, Amir
AU - Kinjo, Yuki
AU - Sullivan, Barbara
AU - Bodmer-Narkevitch, Vera
AU - Plettenburg, Oliver
AU - Kronenberg, Mitchell
AU - Tsuji, Moriya
AU - Ho, David D.
AU - Wong, Chi Huey
PY - 2005/2/1
Y1 - 2005/2/1
N2 - The CD1 family of proteins binds self and foreign glycolipids for presentation to CD1-restricted T cells. To identify previously uncharacterized active CD1 ligands, especially those of microbial origin, numerous glycolipids were synthesized and tested for their ability to stimulate mouse and human natural killer T (NKT) cells. They included analogs of the well known NKT cell agonist α-galactosyl ceramide (α-GalCer), bacterial glycolipids, and variations of the self-glycolipid, sulfatide. Bacterial glycolipids, α-galacturonosyl-ceramides from Sphingomonas wittichii, although structurally similar to α-GalCer, have significant differences in the sugar head group as well as the ceramide portion. The Sphingomonas glycosphingolipids (GSLs) and sulfatide variants were shown to activate human NKT cells as measured by IL-4 and IFN-γ secretion. Moreover, CD1d-dimer staining revealed human NKT cell reactivity toward these GSLs and to the sulfatides in a fashion comparable with α-GalCer. Because α-GalCer is a marine-sponge-derived ligand, our study here shows that bacterium-derived antigens are also able to stimulate mouse and human NKT cells.
AB - The CD1 family of proteins binds self and foreign glycolipids for presentation to CD1-restricted T cells. To identify previously uncharacterized active CD1 ligands, especially those of microbial origin, numerous glycolipids were synthesized and tested for their ability to stimulate mouse and human natural killer T (NKT) cells. They included analogs of the well known NKT cell agonist α-galactosyl ceramide (α-GalCer), bacterial glycolipids, and variations of the self-glycolipid, sulfatide. Bacterial glycolipids, α-galacturonosyl-ceramides from Sphingomonas wittichii, although structurally similar to α-GalCer, have significant differences in the sugar head group as well as the ceramide portion. The Sphingomonas glycosphingolipids (GSLs) and sulfatide variants were shown to activate human NKT cells as measured by IL-4 and IFN-γ secretion. Moreover, CD1d-dimer staining revealed human NKT cell reactivity toward these GSLs and to the sulfatides in a fashion comparable with α-GalCer. Because α-GalCer is a marine-sponge-derived ligand, our study here shows that bacterium-derived antigens are also able to stimulate mouse and human NKT cells.
UR - http://www.scopus.com/inward/record.url?scp=13444255993&partnerID=8YFLogxK
U2 - 10.1073/pnas.0408696102
DO - 10.1073/pnas.0408696102
M3 - Article
C2 - 15665086
AN - SCOPUS:13444255993
VL - 102
SP - 1351
EP - 1356
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 5
ER -