Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 259-269 |
| Seitenumfang | 11 |
| Fachzeitschrift | CYTOTECHNOLOGY |
| Jahrgang | 15 |
| Ausgabenummer | 1-3 |
| Publikationsstatus | Veröffentlicht - Feb. 1994 |
| Extern publiziert | Ja |
Abstract
Two different automated immunoanalysis systems are presented. Both are based on the principles of flow-injection analysis and were developed to provide reliable, rapid monitoring of relevant proteins in animal cell cultivation processes. One system uses a turbidimetric analysis, and the other employs a heterogeneous chemistry with immobilized immunocomponents. For both systems, the analysis time is in the range of a few minutes, and a complete analysis cycle, including triplicate analyses and various washing steps, is in the range of 20-30 minutes. Samples from cultivation processes can be analyzed directly without dilution. Quantitation of proteins such as rt-PA or monoclonal antibodies can be performed over an analyte concentration range of 1-1000 mg/L. Both systems were compared to conventional ELISA assays on microtiter plates. The turbidimetric analysis system also included a biosensor for simultaneous glucose determination.
ASJC Scopus Sachgebiete
- Biochemie, Genetik und Molekularbiologie (insg.)
- Biotechnologie
- Chemische Verfahrenstechnik (insg.)
- Bioengineering
- Ingenieurwesen (insg.)
- Biomedizintechnik
- Biochemie, Genetik und Molekularbiologie (insg.)
- Klinische Biochemie
- Biochemie, Genetik und Molekularbiologie (insg.)
- Zellbiologie
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in: CYTOTECHNOLOGY, Jahrgang 15, Nr. 1-3, 02.1994, S. 259-269.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Automated imunoanalysis systems for monitoring mammalian cell cultivation processes
AU - Schulze, Birgitt
AU - Middendorf, Cornelia
AU - Reinecke, Martin
AU - Scheper, Thomas
AU - Noé, Wolfgang
AU - Howaldt, Michael
PY - 1994/2
Y1 - 1994/2
N2 - Two different automated immunoanalysis systems are presented. Both are based on the principles of flow-injection analysis and were developed to provide reliable, rapid monitoring of relevant proteins in animal cell cultivation processes. One system uses a turbidimetric analysis, and the other employs a heterogeneous chemistry with immobilized immunocomponents. For both systems, the analysis time is in the range of a few minutes, and a complete analysis cycle, including triplicate analyses and various washing steps, is in the range of 20-30 minutes. Samples from cultivation processes can be analyzed directly without dilution. Quantitation of proteins such as rt-PA or monoclonal antibodies can be performed over an analyte concentration range of 1-1000 mg/L. Both systems were compared to conventional ELISA assays on microtiter plates. The turbidimetric analysis system also included a biosensor for simultaneous glucose determination.
AB - Two different automated immunoanalysis systems are presented. Both are based on the principles of flow-injection analysis and were developed to provide reliable, rapid monitoring of relevant proteins in animal cell cultivation processes. One system uses a turbidimetric analysis, and the other employs a heterogeneous chemistry with immobilized immunocomponents. For both systems, the analysis time is in the range of a few minutes, and a complete analysis cycle, including triplicate analyses and various washing steps, is in the range of 20-30 minutes. Samples from cultivation processes can be analyzed directly without dilution. Quantitation of proteins such as rt-PA or monoclonal antibodies can be performed over an analyte concentration range of 1-1000 mg/L. Both systems were compared to conventional ELISA assays on microtiter plates. The turbidimetric analysis system also included a biosensor for simultaneous glucose determination.
KW - Automated immunoanalysis
KW - flow-injection analysis
KW - heterogeneous assay
KW - process analysis
KW - turbidimetric assay
UR - http://www.scopus.com/inward/record.url?scp=0028693802&partnerID=8YFLogxK
U2 - 10.1007/BF00762401
DO - 10.1007/BF00762401
M3 - Article
C2 - 7765939
AN - SCOPUS:0028693802
VL - 15
SP - 259
EP - 269
JO - CYTOTECHNOLOGY
JF - CYTOTECHNOLOGY
SN - 0920-9069
IS - 1-3
ER -