TY - JOUR

T1 - Arbuscular mycorrhizal fungi and goethite promote carbon sequestration via hyphal-aggregate mineral interactions

AU - Jeewani, Peduruhewa H.

AU - Luo, Yu

AU - Yu, Guanghui

AU - Fu, Yingyi

AU - He, Xinhua

AU - Van Zwieten, Lukas

AU - Liang, Chao

AU - Kumar, Amit

AU - He, Yan

AU - Kuzyakov, Yakov

AU - Qin, Hua

AU - Guggenberger, Georg

AU - Xu, Jianming

N1 - Funding Information: This study was supported by the National Natural Science Foundation of China (41671233, U1901601), Zhejiang Outstanding Youth Fund (R19D010005), the Competitive Growth Program of Kazan Federal University, Russia and the ?RUDN University program 5?100?. We thank Dr. Xiaojie Zhou at the BL01B beamline of the National Center for Protein Science Shanghai (NCPSS) at Shanghai Synchrotron Radiation Facility for assistance during SR-FTIR data collection.

PY - 2021/11

Y1 - 2021/11

N2 - Goethite is known to contribute to the co-precipitation of rhizodeposits and thus benefit carbon (C) sequestration, while arbuscular mycorrhizal fungi (AMF) play significant role in soil organic C (SOC), however, the combined effect is less known. To address this paucity in knowledge, we compared the physicochemical stabilization and microbial mineralization of rhizodeposits from maize (Zea mays L.) and the rhizosphere priming effect (RPE) in soils with a combination of goethite addition and AMF inoculation. Here, we showed that compared to the control: i) Co-amendment of AMF and goethite resulted in a 0.6-fold decrease of rhizodeposit derived CO2, and a 2.8-fold larger allocation of rhizodeposits into macro-aggregates, most likely due to precipitation by goethite and macro-aggregate formation stimulated by AMF hyphae. Analyses using μ-FTIR confirmed the spatial distribution of polysaccharides overlapped with Fe–O minerals within macro-aggregates, supporting the concomitant processes of rhizodeposit stabilization and aggregate formation via hyphal-aggregate mineral interactions; ii) Inoculation with AMF accelerated SOC turnover by increasing the RPE (by 6.1 mg C kg−1 day−1, 74% increase) and rhizodeposit stabilization (by 6.2 mg C kg−1 soil day−1, 47% increase). The larger soil priming effect stimulated by AMF was associated with several genera including Solirubrobacter, Pseudomonas and Talaromyces, suggesting these hyper-symbionts were involved in nutrient acquisition (mining hypothesis). Our results enabled the comparison between rhizodeposit stabilization versus rhizodeposit and SOC mineralization, and highlighted the contributions of both goethite (abiotic contribution) and AMF (biotic contribution) to C accrual in a soil-plant system.

AB - Goethite is known to contribute to the co-precipitation of rhizodeposits and thus benefit carbon (C) sequestration, while arbuscular mycorrhizal fungi (AMF) play significant role in soil organic C (SOC), however, the combined effect is less known. To address this paucity in knowledge, we compared the physicochemical stabilization and microbial mineralization of rhizodeposits from maize (Zea mays L.) and the rhizosphere priming effect (RPE) in soils with a combination of goethite addition and AMF inoculation. Here, we showed that compared to the control: i) Co-amendment of AMF and goethite resulted in a 0.6-fold decrease of rhizodeposit derived CO2, and a 2.8-fold larger allocation of rhizodeposits into macro-aggregates, most likely due to precipitation by goethite and macro-aggregate formation stimulated by AMF hyphae. Analyses using μ-FTIR confirmed the spatial distribution of polysaccharides overlapped with Fe–O minerals within macro-aggregates, supporting the concomitant processes of rhizodeposit stabilization and aggregate formation via hyphal-aggregate mineral interactions; ii) Inoculation with AMF accelerated SOC turnover by increasing the RPE (by 6.1 mg C kg−1 day−1, 74% increase) and rhizodeposit stabilization (by 6.2 mg C kg−1 soil day−1, 47% increase). The larger soil priming effect stimulated by AMF was associated with several genera including Solirubrobacter, Pseudomonas and Talaromyces, suggesting these hyper-symbionts were involved in nutrient acquisition (mining hypothesis). Our results enabled the comparison between rhizodeposit stabilization versus rhizodeposit and SOC mineralization, and highlighted the contributions of both goethite (abiotic contribution) and AMF (biotic contribution) to C accrual in a soil-plant system.

KW - C natural abundance

KW - AMF

KW - Carbon sequestration

KW - Rhizodeposition

KW - Rhizosphere priming effects

KW - Synchrotron-radiation-based spectro-microscopy

UR - http://www.scopus.com/inward/record.url?scp=85114690915&partnerID=8YFLogxK

U2 - 10.1016/j.soilbio.2021.108417

DO - 10.1016/j.soilbio.2021.108417

M3 - Article

AN - SCOPUS:85114690915

VL - 162

JO - Soil Biology and Biochemistry

JF - Soil Biology and Biochemistry

SN - 0038-0717

M1 - 108417

ER -