TY - JOUR

T1 - Aptamers vs. antibodies as capture probes in optical porous silicon biosensors

AU - Arshavsky-Graham, Sofia

AU - Urmann, Katharina

AU - Salama, Rachel

AU - Massad-Ivanir, Naama

AU - Walter, Johanna Gabriela

AU - Scheper, Thomas

AU - Segal, Ester

N1 - Funding Information: This work was funded by the German Research Foundation under the grant SCHE 279/32-1. We thank Prof. Ayelet Fishman and her group at the Department for Biotechnology and Food Engineering at the Technion Israel Institute of Technology, for supplying target proteins. ES, SAG, NM and KU acknowledge the core services and support from the Lorry I. Lokey Center for Life Science and Engineering. The authors also wish to thank the student Sharon Nissinmann for her help in some of the biosensing experiments. SAG is most grateful for the Russell Berrie Nanotechnology Excellence Scholarship for Graduate Students.

PY - 2020/6/4

Y1 - 2020/6/4

N2 - Over the past decade aptamers have emerged as a promising class of bioreceptors for biosensing applications with significant advantages over conventional antibodies. However, experimental studies comparing aptasensors and immunosensors, under equivalent conditions, are limited and the results are inconclusive, in terms of benefits and limitations of each bioreceptor type. In the present work, the performance of aptamer and antibody bioreceptors for the detection of a his-tagged protein, used as a model target, is compared. The bioreceptors are immobilized onto a nanostructured porous silicon (PSi) thin film, used as the optical transducer, and the target protein is detected in a real-time and label-free format by reflective interferometric Fourier transform spectroscopy. For the antibodies, random-oriented immobilization onto the PSi nanostructure results in a poor biosensing performance. Contrary, Fc-oriented immobilization of the antibodies shows a similar biosensing performance to that exhibited by the aptamer-based biosensor, in terms of binding rate, dynamic detection range, limit of detection and selectivity. The aptasensor outperforms in terms of its reusability and storability, while the immunosensor could not be regenerated for subsequent experiments.

AB - Over the past decade aptamers have emerged as a promising class of bioreceptors for biosensing applications with significant advantages over conventional antibodies. However, experimental studies comparing aptasensors and immunosensors, under equivalent conditions, are limited and the results are inconclusive, in terms of benefits and limitations of each bioreceptor type. In the present work, the performance of aptamer and antibody bioreceptors for the detection of a his-tagged protein, used as a model target, is compared. The bioreceptors are immobilized onto a nanostructured porous silicon (PSi) thin film, used as the optical transducer, and the target protein is detected in a real-time and label-free format by reflective interferometric Fourier transform spectroscopy. For the antibodies, random-oriented immobilization onto the PSi nanostructure results in a poor biosensing performance. Contrary, Fc-oriented immobilization of the antibodies shows a similar biosensing performance to that exhibited by the aptamer-based biosensor, in terms of binding rate, dynamic detection range, limit of detection and selectivity. The aptasensor outperforms in terms of its reusability and storability, while the immunosensor could not be regenerated for subsequent experiments.

UR - http://www.scopus.com/inward/record.url?scp=85088487464&partnerID=8YFLogxK

U2 - 10.1039/d0an00178c

DO - 10.1039/d0an00178c

M3 - Article

C2 - 32519701

AN - SCOPUS:85088487464

VL - 145

SP - 4991

EP - 5003

JO - The analyst

JF - The analyst

SN - 0003-2654

IS - 14

ER -