Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 2671-2675 |
| Seitenumfang | 5 |
| Fachzeitschrift | Journal of Chromatography A |
| Jahrgang | 1216 |
| Ausgabenummer | 13 |
| Publikationsstatus | Veröffentlicht - 4 Okt. 2008 |
Abstract
The aim of this study was to develop a chromatographic method, as a substitute for enzyme-linked immunosorbent assays, for the rapid and simultaneous detection of IgG, insulin, and transferrin present in a cell culture medium. Conjoint liquid chromatography (conjoint LC) using monolithic disks was applied for this purpose. An anion-exchange disk was combined with a Protein G affinity disk in a preparative HPLC system. IgG bound to the Protein G disk, whereas transferrin and insulin were captured on the quaternary ammonium (QA) disk. Using this method, it was possible to simultaneously determine the concentrations of IgG, transferrin, and insulin in the cell culture medium. Thus, conjoint LC could be used for the rapid and simultaneous detection of different proteins present in a cell culture medium.
ASJC Scopus Sachgebiete
- Chemie (insg.)
- Analytische Chemie
- Biochemie, Genetik und Molekularbiologie (insg.)
- Biochemie
- Chemie (insg.)
- Organische Chemie
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in: Journal of Chromatography A, Jahrgang 1216, Nr. 13, 04.10.2008, S. 2671-2675.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Application of conjoint liquid chromatography with monolithic disks for the simultaneous determination of immunoglobulin G and other proteins present in a cell culture medium
AU - Ralla, Kathrin
AU - Anton, Fabienne
AU - Scheper, Thomas
AU - Kasper, Cornelia
PY - 2008/10/4
Y1 - 2008/10/4
N2 - The aim of this study was to develop a chromatographic method, as a substitute for enzyme-linked immunosorbent assays, for the rapid and simultaneous detection of IgG, insulin, and transferrin present in a cell culture medium. Conjoint liquid chromatography (conjoint LC) using monolithic disks was applied for this purpose. An anion-exchange disk was combined with a Protein G affinity disk in a preparative HPLC system. IgG bound to the Protein G disk, whereas transferrin and insulin were captured on the quaternary ammonium (QA) disk. Using this method, it was possible to simultaneously determine the concentrations of IgG, transferrin, and insulin in the cell culture medium. Thus, conjoint LC could be used for the rapid and simultaneous detection of different proteins present in a cell culture medium.
AB - The aim of this study was to develop a chromatographic method, as a substitute for enzyme-linked immunosorbent assays, for the rapid and simultaneous detection of IgG, insulin, and transferrin present in a cell culture medium. Conjoint liquid chromatography (conjoint LC) using monolithic disks was applied for this purpose. An anion-exchange disk was combined with a Protein G affinity disk in a preparative HPLC system. IgG bound to the Protein G disk, whereas transferrin and insulin were captured on the quaternary ammonium (QA) disk. Using this method, it was possible to simultaneously determine the concentrations of IgG, transferrin, and insulin in the cell culture medium. Thus, conjoint LC could be used for the rapid and simultaneous detection of different proteins present in a cell culture medium.
KW - CIM disk
KW - Conjoint liquid chromatography (conjoint LC)
KW - IgG
KW - Monolith
KW - Protein G
UR - http://www.scopus.com/inward/record.url?scp=61449097614&partnerID=8YFLogxK
U2 - 10.1016/j.chroma.2008.09.102
DO - 10.1016/j.chroma.2008.09.102
M3 - Article
C2 - 18945433
AN - SCOPUS:61449097614
VL - 1216
SP - 2671
EP - 2675
JO - Journal of Chromatography A
JF - Journal of Chromatography A
SN - 0021-9673
IS - 13
ER -