Application of collagen matrices for cartilage tissue engineering

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Autoren

  • Yvonne Stark
  • Kirstin Suck
  • Cornelia Kasper
  • Martin Wieland
  • Martijn Van Griensven
  • Thomas Scheper

Organisationseinheiten

Externe Organisationen

  • Dr. Suwelack Skin and Health Care AG
  • Ludwig Boltzmann Institute for Experimental and Clinical Traumatology
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Details

OriginalspracheEnglisch
Seiten (von - bis)305-311
Seitenumfang7
FachzeitschriftExperimental and Toxicologic Pathology
Jahrgang57
Ausgabenummer4
PublikationsstatusVeröffentlicht - 18 Jan. 2006

Abstract

Articular cartilage shows little capacity for self-repair once it has been damaged. The aim of this study was to investigate different collagen matrices regarding their applicability for cartilage tissue engineering. The matrices consist of collagen I and small amounts of elastine, were crosslinked with carbodiimide or glucose. Primary chondrocytes were seeded onto these different collagen matrices and cultured with or without differentiation medium. The viability of the cells was monitored via MTT test. The arrangement of the cells onto the scaffold was investigated by histological staining. Furthermore, extracellular matrix synthesis was studied by immunohistological staining, especially the expression of the typical chondrogenic marker collagen II. Moreover gene expression for collagen type II was analysed by RT-PCR. The chondrocytes showed high viability on all matrices used. The results for the histological staining revealed a three-dimensional arrangement of the chondrocytes in the collagen matrices. Moreover, the matrices also supported chondrogenic differentiation. On the matrix MATRIDERM® 2 mm the synthesis of collagen II was stimulated without adding any differentiation supplements to the cell culture medium, as observed by immunohistological staining and by gene expression analysis of collagen II.

ASJC Scopus Sachgebiete

Zitieren

Application of collagen matrices for cartilage tissue engineering. / Stark, Yvonne; Suck, Kirstin; Kasper, Cornelia et al.
in: Experimental and Toxicologic Pathology, Jahrgang 57, Nr. 4, 18.01.2006, S. 305-311.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Stark Y, Suck K, Kasper C, Wieland M, Van Griensven M, Scheper T. Application of collagen matrices for cartilage tissue engineering. Experimental and Toxicologic Pathology. 2006 Jan 18;57(4):305-311. doi: 10.1016/j.etp.2005.10.005
Stark, Yvonne ; Suck, Kirstin ; Kasper, Cornelia et al. / Application of collagen matrices for cartilage tissue engineering. in: Experimental and Toxicologic Pathology. 2006 ; Jahrgang 57, Nr. 4. S. 305-311.
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AU - Stark, Yvonne

AU - Suck, Kirstin

AU - Kasper, Cornelia

AU - Wieland, Martin

AU - Van Griensven, Martijn

AU - Scheper, Thomas

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AB - Articular cartilage shows little capacity for self-repair once it has been damaged. The aim of this study was to investigate different collagen matrices regarding their applicability for cartilage tissue engineering. The matrices consist of collagen I and small amounts of elastine, were crosslinked with carbodiimide or glucose. Primary chondrocytes were seeded onto these different collagen matrices and cultured with or without differentiation medium. The viability of the cells was monitored via MTT test. The arrangement of the cells onto the scaffold was investigated by histological staining. Furthermore, extracellular matrix synthesis was studied by immunohistological staining, especially the expression of the typical chondrogenic marker collagen II. Moreover gene expression for collagen type II was analysed by RT-PCR. The chondrocytes showed high viability on all matrices used. The results for the histological staining revealed a three-dimensional arrangement of the chondrocytes in the collagen matrices. Moreover, the matrices also supported chondrogenic differentiation. On the matrix MATRIDERM® 2 mm the synthesis of collagen II was stimulated without adding any differentiation supplements to the cell culture medium, as observed by immunohistological staining and by gene expression analysis of collagen II.

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