Antimicrobial properties and biocompatibility of semi-synthetic carbohydrate-based ionic hydrogels

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Autoren

Organisationseinheiten

Externe Organisationen

  • Universität Rostock
Forschungs-netzwerk anzeigen

Details

OriginalspracheEnglisch
Seiten (von - bis)30719-30731
Seitenumfang13
FachzeitschriftRSC Advances
Jahrgang14
Ausgabenummer42
Frühes Online-Datum26 Sept. 2024
PublikationsstatusVeröffentlicht - 2024

Abstract

Hydrogels have gained significant interest in the last decades, especially in the medical sector, due to their versatile properties. While hydrogels from naturally occurring polysaccharides (e.g. cellulose) are well-known, those produced from polymerizable carbohydrate-based monomers remain underexplored. However, these semi-synthetic hydrogels offer the great advantage of having adjustable properties for customization depending on their application. The objective of this study was to characterize semi-synthetic carbohydrate-based ionic hydrogels produced from GVIM-I (glucosyl vinyl imidazolium iodide). The antimicrobial activity was evaluated using the disk diffusion method, which demonstrated that all samples exhibit inhibitory effects on the growth of Candida auris. In vitro biocompatibility was determined by cell viability studies with L929 mouse fibroblasts, and a correlation was observed between eluate concentration and cell viability. In particular, the type of initiator system employed for polymerization was found to affect cell viability. The direct contact assessments showed that specific pre-treatments of the hydrogels resulted in higher cell viability than non-treated hydrogels. The results also revealed the impact of crosslinker concentration and type and identified poly(ethylene glycol)diacrylate (PEGDA) 575 as a promising crosslinker for future medical applications. LC-MS analysis of the wash medium identified unreacted GVIM-I as the leached material, which is presumed to be the cause of the observed cytotoxicity. Overall, the study provides valuable insights into the characteristics of GVIM-I based hydrogels and sheds light on the factors that influence their cytotoxicity and potential for medical application.

ASJC Scopus Sachgebiete

Zitieren

Antimicrobial properties and biocompatibility of semi-synthetic carbohydrate-based ionic hydrogels. / Lambrecht, Sina; Gazizova, Alina; Kara, Selin et al.
in: RSC Advances, Jahrgang 14, Nr. 42, 2024, S. 30719-30731.

Publikation: Beitrag in FachzeitschriftArtikelForschungPeer-Review

Lambrecht S, Gazizova A, Kara S, Meyer J, Jopp S. Antimicrobial properties and biocompatibility of semi-synthetic carbohydrate-based ionic hydrogels. RSC Advances. 2024;14(42):30719-30731. Epub 2024 Sep 26. doi: 10.1039/D4RA05695G
Lambrecht, Sina ; Gazizova, Alina ; Kara, Selin et al. / Antimicrobial properties and biocompatibility of semi-synthetic carbohydrate-based ionic hydrogels. in: RSC Advances. 2024 ; Jahrgang 14, Nr. 42. S. 30719-30731.
Download
@article{a68789bafde44ed1b103bf48b5fec429,
title = "Antimicrobial properties and biocompatibility of semi-synthetic carbohydrate-based ionic hydrogels",
abstract = "Hydrogels have gained significant interest in the last decades, especially in the medical sector, due to their versatile properties. While hydrogels from naturally occurring polysaccharides (e.g. cellulose) are well-known, those produced from polymerizable carbohydrate-based monomers remain underexplored. However, these semi-synthetic hydrogels offer the great advantage of having adjustable properties for customization depending on their application. The objective of this study was to characterize semi-synthetic carbohydrate-based ionic hydrogels produced from GVIM-I (glucosyl vinyl imidazolium iodide). The antimicrobial activity was evaluated using the disk diffusion method, which demonstrated that all samples exhibit inhibitory effects on the growth of Candida auris. In vitro biocompatibility was determined by cell viability studies with L929 mouse fibroblasts, and a correlation was observed between eluate concentration and cell viability. In particular, the type of initiator system employed for polymerization was found to affect cell viability. The direct contact assessments showed that specific pre-treatments of the hydrogels resulted in higher cell viability than non-treated hydrogels. The results also revealed the impact of crosslinker concentration and type and identified poly(ethylene glycol)diacrylate (PEGDA) 575 as a promising crosslinker for future medical applications. LC-MS analysis of the wash medium identified unreacted GVIM-I as the leached material, which is presumed to be the cause of the observed cytotoxicity. Overall, the study provides valuable insights into the characteristics of GVIM-I based hydrogels and sheds light on the factors that influence their cytotoxicity and potential for medical application.",
author = "Sina Lambrecht and Alina Gazizova and Selin Kara and Johanna Meyer and Stefan Jopp",
note = "Publisher Copyright: {\textcopyright} 2024 The Royal Society of Chemistry.",
year = "2024",
doi = "10.1039/D4RA05695G",
language = "English",
volume = "14",
pages = "30719--30731",
journal = "RSC Advances",
issn = "2046-2069",
publisher = "Royal Society of Chemistry",
number = "42",

}

Download

TY - JOUR

T1 - Antimicrobial properties and biocompatibility of semi-synthetic carbohydrate-based ionic hydrogels

AU - Lambrecht, Sina

AU - Gazizova, Alina

AU - Kara, Selin

AU - Meyer, Johanna

AU - Jopp, Stefan

N1 - Publisher Copyright: © 2024 The Royal Society of Chemistry.

PY - 2024

Y1 - 2024

N2 - Hydrogels have gained significant interest in the last decades, especially in the medical sector, due to their versatile properties. While hydrogels from naturally occurring polysaccharides (e.g. cellulose) are well-known, those produced from polymerizable carbohydrate-based monomers remain underexplored. However, these semi-synthetic hydrogels offer the great advantage of having adjustable properties for customization depending on their application. The objective of this study was to characterize semi-synthetic carbohydrate-based ionic hydrogels produced from GVIM-I (glucosyl vinyl imidazolium iodide). The antimicrobial activity was evaluated using the disk diffusion method, which demonstrated that all samples exhibit inhibitory effects on the growth of Candida auris. In vitro biocompatibility was determined by cell viability studies with L929 mouse fibroblasts, and a correlation was observed between eluate concentration and cell viability. In particular, the type of initiator system employed for polymerization was found to affect cell viability. The direct contact assessments showed that specific pre-treatments of the hydrogels resulted in higher cell viability than non-treated hydrogels. The results also revealed the impact of crosslinker concentration and type and identified poly(ethylene glycol)diacrylate (PEGDA) 575 as a promising crosslinker for future medical applications. LC-MS analysis of the wash medium identified unreacted GVIM-I as the leached material, which is presumed to be the cause of the observed cytotoxicity. Overall, the study provides valuable insights into the characteristics of GVIM-I based hydrogels and sheds light on the factors that influence their cytotoxicity and potential for medical application.

AB - Hydrogels have gained significant interest in the last decades, especially in the medical sector, due to their versatile properties. While hydrogels from naturally occurring polysaccharides (e.g. cellulose) are well-known, those produced from polymerizable carbohydrate-based monomers remain underexplored. However, these semi-synthetic hydrogels offer the great advantage of having adjustable properties for customization depending on their application. The objective of this study was to characterize semi-synthetic carbohydrate-based ionic hydrogels produced from GVIM-I (glucosyl vinyl imidazolium iodide). The antimicrobial activity was evaluated using the disk diffusion method, which demonstrated that all samples exhibit inhibitory effects on the growth of Candida auris. In vitro biocompatibility was determined by cell viability studies with L929 mouse fibroblasts, and a correlation was observed between eluate concentration and cell viability. In particular, the type of initiator system employed for polymerization was found to affect cell viability. The direct contact assessments showed that specific pre-treatments of the hydrogels resulted in higher cell viability than non-treated hydrogels. The results also revealed the impact of crosslinker concentration and type and identified poly(ethylene glycol)diacrylate (PEGDA) 575 as a promising crosslinker for future medical applications. LC-MS analysis of the wash medium identified unreacted GVIM-I as the leached material, which is presumed to be the cause of the observed cytotoxicity. Overall, the study provides valuable insights into the characteristics of GVIM-I based hydrogels and sheds light on the factors that influence their cytotoxicity and potential for medical application.

UR - http://www.scopus.com/inward/record.url?scp=85205445519&partnerID=8YFLogxK

U2 - 10.1039/D4RA05695G

DO - 10.1039/D4RA05695G

M3 - Article

VL - 14

SP - 30719

EP - 30731

JO - RSC Advances

JF - RSC Advances

SN - 2046-2069

IS - 42

ER -

Von denselben Autoren