Details
| Originalsprache | Englisch |
|---|---|
| Seiten (von - bis) | 2642-2646 |
| Seitenumfang | 5 |
| Fachzeitschrift | Natural product research |
| Jahrgang | 34 |
| Ausgabenummer | 18 |
| Frühes Online-Datum | 24 Dez. 2018 |
| Publikationsstatus | Veröffentlicht - 16 Sept. 2020 |
Abstract
Using a dual culture antagonism assay, Aspergillus niger exhibited 51.5 ± 1.1% growth inhibition against Fusarium oxysporum f.sp. lycopersici, the wilt pathogen of tomato. For enhanced production of antifungal metabolites, nutrient optimization was performed and in vitro well-diffusion antifungal assays demonstrated that crude extract obtained from GPYB culture showed a maximum zone of inhibition (8.8 ± 0.4 mm) against the wilt pathogen, which is corroborated by the comparative LCMS profiles of the extracts from all three media i.e. GPYB, YEB and PDB. Two known compounds, Asperazine (m/z 665 [M + H]+) and Nigerone (m/z 571 [M + H]+), were isolated from A. niger and their antifungal activity is reported here for the first time. In MIC experiments, Asperazine and Nigerone inhibited the pathogen at 60 and 80 µg·mL−1 respectively. Molecular docking studies of Nigerone and Asperazine with F. oxysporum tomatinase showed five and six binding interactions respectively.
ASJC Scopus Sachgebiete
- Chemie (insg.)
- Analytische Chemie
- Biochemie, Genetik und Molekularbiologie (insg.)
- Biochemie
- Agrar- und Biowissenschaften (insg.)
- Pflanzenkunde
- Chemie (insg.)
- Organische Chemie
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in: Natural product research, Jahrgang 34, Nr. 18, 16.09.2020, S. 2642-2646.
Publikation: Beitrag in Fachzeitschrift › Artikel › Forschung › Peer-Review
}
TY - JOUR
T1 - Antifungal activity of compounds isolated from Aspergillus niger and their molecular docking studies with tomatinase
AU - Zulqarnain,
AU - Iqbal, Zafar
AU - Cox, Russell
AU - Anwar, Jawad
AU - Ahmad, Nasir
AU - Khan, Khalid
AU - Iqbal, Mudassar
AU - Manzoor, Nazish
AU - Khattak, Saeed Ullah
N1 - Funding information: This work was supported by Pakistan Science Foundation (PSF), Islamabad under Project No. PSF/NSLP/KP-AU (421); International Research Support Initiative Program of Higher Education Commission (HEC), Pakistan.
PY - 2020/9/16
Y1 - 2020/9/16
N2 - Using a dual culture antagonism assay, Aspergillus niger exhibited 51.5 ± 1.1% growth inhibition against Fusarium oxysporum f.sp. lycopersici, the wilt pathogen of tomato. For enhanced production of antifungal metabolites, nutrient optimization was performed and in vitro well-diffusion antifungal assays demonstrated that crude extract obtained from GPYB culture showed a maximum zone of inhibition (8.8 ± 0.4 mm) against the wilt pathogen, which is corroborated by the comparative LCMS profiles of the extracts from all three media i.e. GPYB, YEB and PDB. Two known compounds, Asperazine (m/z 665 [M + H]+) and Nigerone (m/z 571 [M + H]+), were isolated from A. niger and their antifungal activity is reported here for the first time. In MIC experiments, Asperazine and Nigerone inhibited the pathogen at 60 and 80 µg·mL−1 respectively. Molecular docking studies of Nigerone and Asperazine with F. oxysporum tomatinase showed five and six binding interactions respectively.
AB - Using a dual culture antagonism assay, Aspergillus niger exhibited 51.5 ± 1.1% growth inhibition against Fusarium oxysporum f.sp. lycopersici, the wilt pathogen of tomato. For enhanced production of antifungal metabolites, nutrient optimization was performed and in vitro well-diffusion antifungal assays demonstrated that crude extract obtained from GPYB culture showed a maximum zone of inhibition (8.8 ± 0.4 mm) against the wilt pathogen, which is corroborated by the comparative LCMS profiles of the extracts from all three media i.e. GPYB, YEB and PDB. Two known compounds, Asperazine (m/z 665 [M + H]+) and Nigerone (m/z 571 [M + H]+), were isolated from A. niger and their antifungal activity is reported here for the first time. In MIC experiments, Asperazine and Nigerone inhibited the pathogen at 60 and 80 µg·mL−1 respectively. Molecular docking studies of Nigerone and Asperazine with F. oxysporum tomatinase showed five and six binding interactions respectively.
KW - antifungal docking
KW - Dual culture assay
KW - fungicides
KW - growth inhibition
KW - LCMS
UR - http://www.scopus.com/inward/record.url?scp=85058999315&partnerID=8YFLogxK
U2 - 10.1080/14786419.2018.1548447
DO - 10.1080/14786419.2018.1548447
M3 - Article
AN - SCOPUS:85058999315
VL - 34
SP - 2642
EP - 2646
JO - Natural product research
JF - Natural product research
SN - 1478-6419
IS - 18
ER -